Extract of antineoplastic active components in vietnamese sophora root, and preparation method and application thereof

A technology of active components and anti-tumor drugs, applied in the field of anti-tumor effective component extracts of mountain bean root and its preparation method and application, can solve the problems of no in vitro anti-tumor experiments, etc., to expand the use and increase the added value Effect

Active Publication Date: 2015-02-18
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent extract is different from the present invention, and there is no in vitro anti-tumor experiment of the extract on U251 cells

Method used

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  • Extract of antineoplastic active components in vietnamese sophora root, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1. The preparation of the anti-tumor effective component extract of the mountain bean root

[0022] Dry and pulverize the bean root, take 500g of the medicinal powder, reflux extraction with 10 times the mass of water for 2 hours, repeat the extraction of the centrifuged residue once, centrifuge, combine the secondary water extracts, concentrate to 300ml, add 95% ethanol for alcohol precipitation , the concentration of ethanol in the medicinal liquid is about 60%, let it stand overnight, centrifuge, discard the precipitate, recover ethanol under reduced pressure, continue to concentrate to 150ml, add an equal volume of ethyl acetate to extract 4 times, combine the extracts, concentrate, vacuum After drying, about 8.21 g of ethyl acetate extract was obtained.

[0023] The above-mentioned ethyl acetate extract was passed through a silica gel column, and 300ml of dichloromethane and methanol mixtures (100:1, 50:1, 20:1, 10:1, 5:1, 1:1) in different proportions w...

Embodiment 2

[0024] Example 2. The preparation of the anti-tumor effective component extract of Shandou root

[0025] Dry and pulverize the bean root, take 400g of medicinal powder, reflux extraction with 10 times the amount of water for 2 hours, repeat the extraction of the centrifuged residue once, centrifuge, combine the secondary water extracts, concentrate to 300ml, add 95% ethanol for alcohol precipitation , the concentration of ethanol in the medicinal solution is about 60%, let it stand overnight, centrifuge, discard the precipitate, recover ethanol under reduced pressure, and continue to concentrate to 150ml. Extract with an equal volume of petroleum ether for 3 times, collect the extracted aqueous layer, and then extract with an equal volume of ethyl acetate for 3 times, combine the extracts, concentrate, and dry in vacuo to obtain an ethyl acetate extract. The ethyl acetate extract was separated on a silica gel column, eluted with different ratios of dichloromethane-methanol (10...

Embodiment 3

[0027] Example 3. The preparation of the anti-tumor effective component extract of Shandou root

[0028]Dry and pulverize the bean root, take 400g of medicinal powder, reflux extract with 10 times the mass of 45% ethanol for 3 hours, repeat the extraction of the centrifuged residue once, centrifuge, combine the second alcohol extract, concentrate until it has no alcohol smell, add water to 300ml , centrifuge, discard the precipitate, continue to concentrate to 150ml, add an equal volume of ethyl acetate to extract 4 times, combine the ethyl acetate extracts, recycle the ethyl acetate to obtain the ethyl acetate extract. The ethyl acetate extract was passed through a silica gel column, and 300ml of dichloromethane and methanol mixtures (100:1, 50:1, 20:1, 10:1, 5:1, 1:1) in different proportions were prepared, and the collection ratio The 50:1 eluate was concentrated and vacuum-dried to obtain the antitumor effective component extract of the root of the mountain bean.

[0029]...

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Abstract

The invention discloses an extract of antineoplastic active components in a vietnamese sophora root and a preparation method thereof. The preparation method comprises the following steps: 1, successively carrying out extraction with water, condensation of extracted liquid, alcohol precipitation, centrifugation for removal of a precipitate and reduced pressure condensation for removal of ethanol so as to obtain extracted liquid, or successively carrying out heating extraction with 40 to 95% ethanol in a water-bath with a temperature of 80 DEG C, cooling, centrifugation and condensation for removal of ethanol so as to obtain the extracted liquid; and 2, extracting the extracted liquid with ethyl acetate, subjecting ethyl acetate extracted liquid to separation with a silica gel column, wherein eluents are dichloromethane-methanol with different volume ratios, collecting eluate obtained by using dichloromethane-methanol with a volume ratio of 50: 1 and recovering the organic solvent in the eluate so as to obtain the extract of the antineoplastic active components in the vietnamese sophora root. According to in vitro cytotoxicity experiments, the extract of the antineoplastic active components in the vietnamese sophora root has obvious inhibitory effects on U251 (glioma cells) and can be used for preparing antineoplastic drugs. The invention discloses the preparation method for the extract.

Description

technical field [0001] The present invention relates to the anti-tumor effective component extract of bean root and its preparation method and application. Background technique [0002] At present, due to the intensification of environmental pollution, the acceleration of the pace of life, and the increase in the pressure of life, the incidence of cancer is on the rise. According to the latest data from the World Health Organization, the global incidence of cancer will increase by 50% by 2020. Looking for Effective anti-tumor drugs and methods to completely conquer tumors are important research topics in the world medical community. In recent years, the incidence of cancer in my country has increased at a rate of 2.5% per year, so it is more urgent to speed up the research of anticancer drugs. [0003] In recent years, anti-tumor preparations of traditional Chinese medicine have become a hot spot in the research and development of new drugs, including active ingredients ext...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/489A61P35/00
Inventor 余江南郭林丰徐希明童姗姗张辉云
Owner JIANGSU UNIV
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