Chicken subunit four-combination vaccine and preparation and application thereof

A vaccine and chicken infectious technology, applied in medical preparations containing active ingredients, antibody medical ingredients, antiviral agents, etc., can solve problems such as short immunization period, immunity easily interfered by maternal antibodies, and respiratory side effects

Active Publication Date: 2014-04-09
QINGDAO VLAND BIOTECH INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the Newcastle disease live vaccine is effective, cheap and easy to use, it has a short immunization period and is easily interfered by maternal antibodies, and it is easy to cause respiratory side effects and interfere with laboratory diagnosis of virus isolation. Therefore, since 1980, Newcastle disease inactivated vaccines have been used Vaccine has gradually become one of the routine methods for preventing and controlling Newcastle disease in chicken farms in my country

Method used

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  • Chicken subunit four-combination vaccine and preparation and application thereof
  • Chicken subunit four-combination vaccine and preparation and application thereof
  • Chicken subunit four-combination vaccine and preparation and application thereof

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Embodiment Construction

[0014] The main structural protein VP2 protein of IBDV is the most important host protective antigen. In the present invention, the super-virulent VP2 gene of IBD is amplified by PCR method, cloned into pET-28a vector, transformed into BL21 (DE3), and induced by IPTG. After the VP2 protein is expressed, the expressed cells are homogenized and broken by high pressure, and the supernatant is obtained by centrifugation to obtain the VP2 protein solution.

[0015] Newcastle disease virus Clone30 strain was inoculated into 10-day-old SPF embryos, and the chicken embryo liquid cultured for 48 to 96 hours was collected, and the virus liquid was concentrated by ultrafiltration concentration method, and the final concentration of 0.1% formaldehyde was added.

[0016] Chicken infectious bronchitis virus M42 strain was inoculated into 10-11-day-old SPF embryos, and the chicken embryo liquid cultured for 24-48 hours was collected, the virus was concentrated by ultrafiltration concentration...

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Abstract

The invention aims to prepare a chicken subunit four-combination vaccine which is capable of simultaneously preventing newcastle disease, infectious bronchitis, avian influenza, infectious bursal disease and particularly super-strong virus infection. The vaccine comprises infectious bursal disease VP2 protein with the sequence of SEQ ID NO:1, newcastle disease virus, infectious bronchitis virus and avian influenza virus. The four-combination vaccine can be applied to vaccine immunity of chickens of 21 days, can be used for simultaneously preventing the newcastle disease, the infectious bronchitis, the avian influenza and the infectious bursal disease, has immune effects similar to those of a single newcastle disease vaccine, the inactivated vaccine of the infectious bronchitis, the inactivated vaccine of the avian influenza and single infectious bursal disease vaccine, and fulfills the aims of reducing immunization frequency and lowering stress.

Description

technical field [0001] The invention belongs to the field of avian vaccine manufacturing, in particular to a chicken subunit quadruple vaccine and its preparation and application, namely a chicken Newcastle disease-infectious bronchitis-avian influenza-infectious bursal disease quadruple vaccine. Background technique [0002] Chicken Newcastle disease (ND) is an acute infectious disease of poultry caused by chicken Newcastle disease virus (NDV). Although the live chicken Newcastle disease vaccine is effective, inexpensive and easy to use, its immune period is short, immunity is easily interfered by maternal antibodies, and it is easy to cause respiratory side effects and interfere with laboratory diagnosis of virus isolation. Therefore, since 1980, the use of chicken Newcastle disease inactivated vaccine Vaccines have gradually become one of the routine methods for the prevention and treatment of Newcastle disease in chicken farms in my country. [0003] Infectious Bursal Di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/215A61K39/17A61K39/145A61K39/12A61P31/14A61P31/16
Inventor 王雷凌红丽于春梅吴晓燕
Owner QINGDAO VLAND BIOTECH INC
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