Heat-resisting protective agent for duck virus hepatitis live vaccines and preparation method and application of protective agent

A technology of duck viral hepatitis and heat-resistant protective agent, which is applied in antiviral agents, medical preparations containing active ingredients, pharmaceutical formulas, etc., to overcome backward storage technology, reduce heat source stress, improve vaccine quality and market competition force effect

Active Publication Date: 2013-04-03
SHANGHAI CHUANG HONG BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is exactly to provide a kind of overcoming the present situation that domestic vaccine preserves technology backwardness in order to overcome the defective that above-mentioned prior art exists, the duck virus hepatitis live vaccine that improves vaccine quality and market competitiveness uses heat-resistant protective agent and preparation method thereof and application

Method used

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  • Heat-resisting protective agent for duck virus hepatitis live vaccines and preparation method and application of protective agent
  • Heat-resisting protective agent for duck virus hepatitis live vaccines and preparation method and application of protective agent
  • Heat-resisting protective agent for duck virus hepatitis live vaccines and preparation method and application of protective agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Heat-resistant storage technology of type 1 duck viral hepatitis live vaccine

[0048] (1) Dissolve 5% gelatin and 10% sorbitol in double-distilled water according to the above ratio, and control the temperature at 108-121°C for 30 minutes; , dipotassium hydrogen phosphate 2%, potassium dihydrogen phosphate 0.52% are dissolved in double distilled water according to the above ratio, and are sterilized with a filter membrane of 0.22 μm, and then it is mixed with the components prepared in step (1) by 1:1 Mix to make a heat-resistant protectant.

[0049] (2) After cultured type 1 duck viral hepatitis virus, carry out sterility test and determination of virus content, mix the qualified virus liquid and the prepared freeze-dried protective agent 1:1, shake well, and quantitatively dispense Immediately freeze-dried.

[0050] (3) Put the subpackaged vaccine into the freeze dryer box, slowly cool down to -45°C within 3 hours, and maintain it at this temperature for 6 hours, t...

Embodiment 2

[0052] Heat-resistant storage technology of type 1 duck viral hepatitis live vaccine

[0053] (1) Dissolve 5% gelatin and 10% sorbitol in double-distilled water according to the above ratio, and control the temperature at 108-121°C for 30 minutes to autoclave; 2% urea, 1% fatty acid salt, 1% vitamin C, Monosodium phosphate 3%, dipotassium hydrogen phosphate 2%, and potassium dihydrogen phosphate 0.52% were dissolved in double distilled water according to the above ratio, sterilized with a filter membrane of 0.22 μm, and then it was mixed with the prepared in step (1). The components are mixed at a ratio of 1:1 to prepare a heat-resistant protective agent.

[0054] (2) After cultured type 1 duck viral hepatitis virus, carry out sterility test and determination of virus content, mix the qualified virus liquid and the prepared freeze-dried protective agent 1:1, shake well, and quantitatively dispense Immediately freeze-dried.

[0055] (3) Put the subpackaged vaccine into the fr...

Embodiment 3

[0066] The preparation method of heat-resistant protective agent for type 3 duck viral hepatitis live vaccine comprises the following steps:

[0067] (1) Dissolve 5% gelatin and 10% sorbitol in double-distilled water according to the above ratio, and control the temperature at 108-121°C for high-pressure sterilization for 30 minutes; 2% urea, 1% fatty acid salt, 1% vitamin C, Monosodium glutamate 3%, dipotassium hydrogen phosphate 2%, and potassium dihydrogen phosphate 0.52% were dissolved in double distilled water according to the above ratio, sterilized with a 0.22 μm filter membrane, and then prepared in step (1) The components are mixed at a ratio of 1:1 to prepare a heat-resistant protective agent.

[0068] (2) After the type 3 duck viral hepatitis virus is cultured, carry out the sterility test and the determination of the virus content, mix the qualified virus liquid and the prepared freeze-dried protective agent 1:1, shake well, and quantitatively distribute Immediate...

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Abstract

The invention relates to a heat-resisting protective agent for duck virus hepatitis live vaccines and a preparation method and application of the protective agent. The method comprises the following steps of: mixing and preparing a freeze-dried protective agent matrix in a certain ratio, performing sterile treatment on the protective agent, dissolving the protective agent components for autoclaved sterilization into double distilled water, and sterilizing for 15 to 30 minutes at the temperature of between 108 and 121 DEG C; and dissolving the protective agent components without autoclaved sterilization resistance into double distilled water in a ratio, removing bacteria by using a 0.22mu m filter membrane, mixing the both to form the heat-resisting protective agent, mixing the heat-resisting protective agent and duck virus hepatitis liquid in a ratio of 1: (1-1.2), and performing freeze-drying by adopting a corresponding freeze-drying curve. Compared with the prior art, a good resource is provided for preparation of the duck virus hepatitis live vaccines, the backward status of a domestic vaccine preservation technology is overcome, the live vaccines are preserved for 24 months at the temperature of between 2 and 8 DEG C, and the titers of the vaccines on each feather are kept to be more than or equal to 106.0EID50.

Description

technical field [0001] The invention belongs to the field of production of live vaccines for viral diseases for animals, and in particular relates to a heat-resistant protective agent for duck viral hepatitis live vaccines and a preparation method and application thereof. Background technique [0002] Duck viral hepatitis (Duck Viral Hepatitis, DVH) is an acute infectious disease of ducklings caused by duck viral hepatitis virus (Duck Hepatitis Virus, DHV; belongs to picornavirus), which is highly lethal to ducklings. Seriously affect the development of duck industry. A variety of viruses have been reported clinically to cause viral hepatitis in ducklings. It has been confirmed that duck viral hepatitis virus type 1, new serotype duck viral hepatitis virus and variant strain of duck viral hepatitis virus type 1 of picornaviridae, as well as duck viral hepatitis virus type 2 and type 3 of astroviridae Wait. Since the virus was isolated in Beijing in 1980 in my country and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K39/29A61P31/14
Inventor 苏敬良赖志漆世华高俊锋胡彦斌孙庆歌马晶晶
Owner SHANGHAI CHUANG HONG BIOTECH
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