MiR-3OB (Micro-Ribonucleic Acid-30b) composite for targeting microglia as well as preparation method and application of composite

A technology of microglial cells and complexes, applied in the field of biomedicine, can solve problems such as accelerating central nervous system diseases and nerve cell damage, and achieve the effects of inhibiting occurrence or deterioration, increasing uptake rate, and enhancing transfection efficiency

Inactive Publication Date: 2013-04-03
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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Problems solved by technology

However, studies have shown that overactivated microglia may accelerate the process of some central nervous system diseases, and the cytotoxic factors released by them will also cause further damage to nerve cells

Method used

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  • MiR-3OB (Micro-Ribonucleic Acid-30b) composite for targeting microglia as well as preparation method and application of composite
  • MiR-3OB (Micro-Ribonucleic Acid-30b) composite for targeting microglia as well as preparation method and application of composite
  • MiR-3OB (Micro-Ribonucleic Acid-30b) composite for targeting microglia as well as preparation method and application of composite

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Embodiment Construction

[0016] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.

[0017] 1. Preparation of miR-30b complex targeting microglia

[0018] 1. Preparation of fusion peptide K9-S9 of K9 peptide and S9 peptide

[0019] The fusion peptide K9-S9 is formed by directly linking the carboxyl terminal of K9 peptide and the amino terminal of S9 peptide, and the amino acid sequence is KKKKKK-KKKVVMKFLTQQ (SEQ ID No.1).

[0020] The fusion peptide K9-S9 was synthesized on an AB-431A peptide syn...

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Abstract

The invention discloses a miR-3OB (Micro-Ribonucleic Acid-30b) composite of targeting microglia. The composite is formed by binding a fusion peptide of K9 peptide (KKKKKKKKK) and S9 peptide (VVMKFLTQQ) and the miR-30b by means of electrostatic interaction; the composite can use the guide effect of the S9 peptide to specifically target a specific CX3CR1 receptor on the surface of the microglia, and at the same time when the S9 peptide and the CX3CR1 receptor act with each other, the miR-30b is efficiently transfected into the microglia to inhibit the microglia activation so as to further inhibit the occurrence and progression of a microglia-mediated central nervous system disease. The composite can be applied to the preparation of a medicine for treating the microglia-mediated central nervous system disease and has good development and application prospect in the field of treating the nervous system disease.

Description

technical field [0001] The invention belongs to the field of biomedicine, relates to a polypeptide-gene complex, and also relates to a preparation method of the complex and an application in the field of pharmacy. Background technique [0002] As the first line of defense in the central nervous system, microglia play an important role in the inflammatory response. When foreign pathogens invade or cells die, microglia are quickly activated and eliminated, and at the same time release inflammatory factors to mediate the inflammatory response. However, studies have shown that overactivated microglia may accelerate the process of some central nervous system diseases, and the cytotoxic factors released by them will also cause further damage to nerve cells. Therefore, appropriately inhibiting the activity of microglia may be an effective way to treat central nervous system diseases. [0003] MicroRNA (microRNA, miRNA) is a kind of non-coding RNA molecule of about 21-25 nucleotid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K31/7088A61K47/42A61P25/28
Inventor 杨曌杨清武
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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