First-level strain preservation culture medium for Pleurotus eryngii and preparation method of same

Abstract

The invention discloses a first-level strain preservation culture medium for Pleurotus eryngii and a preparation method of the first-level strain preservation culture medium. The culture medium comprises the following components in part by weight: 75 to 80 parts of broad-leaved tree saw dust; 3 to 6 parts of bagasse, 12 to 18 parts of brans, 2 to 3 parts of acrylamide copolymerization cross-linking agent; 0.5 to 0.8 part of potassium dihydrogen phosphate; and 2 to 3 parts of lime. The preparation process of the culture medium comprises the steps of pretreatment of the broad-leaved tree saw dust, pretreatment of the acrylamide copolymerization cross-linking agent, mixing of the culture medium, bottling of the culture medium and sterilization and inoculation. Under the synergistic action of the components of the culture medium disclosed by the invention, the prepared culture medium can enable a first-level strain of the Pleurotus eryngii to be safely and stably preserved under the conventional conditions and the strain of the Pleurotus eryngii is not degenerated or changed in three years; the raw materials of the culture medium are easy to obtain; the preparation method is simple; the culture medium is suitable for producing edible fungi; and a first-level strain preservation method, which is low in cost, is simple and convenient to operate, is safe and stable and has good effects, is provided for enterprises which continuously produce the edible fungi in a large scale.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a primary culture medium for preserving Pleurotus eryngii and a preparation method thereof. Background technique [0002] At present, in the factory production of Pleurotus eryngii, due to the annual continuous and large-scale production, the strains are also produced continuously and in large quantities. Producers use the three-stage propagation method to produce strains, and the first-grade strains mostly In the method of continuous transfer tube subcultivation, the degradation of the bacteria is more serious or the hidden danger is serious; some producers adopt the simple PDA subculture transfer tube low-temperature preservation method for the first-class bacteria, and the bacteria often appear after one year Degradation or mutation caused serious losses, and good strains could not be effectively preserved; some producers directly purchased strains from outsi...

AI Technical Summary

Problems solved by technology

[0002] At present, in the factory production of Pleurotus eryngii, due to the annual continuous and large-scale production, the strains are also produced continuously and in large quantities. Producers use the three-stage propagation method to produce strains, and the first-grade strains mostly In the method of continuous transfer tube subcultivation, the degradation of the bacteria is more serious or the hidden danger is serious; some producers adopt the simple PDA subculture transfer tube low-temperature preservation method for the first-level bacteria, and the bacteria often appear after one year Degradation or mutation, causing serious losses, good strains cannot be effectively preserved; some producers directly purchase strains from outside, production cannot be guaranteed, and the quality of strains is also unstable