Method for combined extraction of variety of functional components of ginger

A technology of functional components and joint extraction, applied in the separation/purification of carbonyl compounds, hydrolytic enzymes, organic chemistry, etc., can solve problems such as blank, high cost, and small production scale

Active Publication Date: 2013-04-03
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0006] Our country is very rich in ginger resources, but due to the high cost of extracting certain components alone, the production scale is small or even blank. Therefore, the joint extraction of various ginger functional components with high efficiency, lo

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  • Method for combined extraction of variety of functional components of ginger

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Embodiment Construction

[0013] Wash and chop ginger (1) and absolute ethanol (2) according to 1:2~1:4 (w / v) beating (3), centrifuge at 3000~5000rpm (4) for 5~10min, get Supernatant (5) and Precipitate (6). The supernatant (5) is vacuum rotary evaporated (7) at 40-60°C and 16-48rpm, and the rotary evaporated liquid (8) is water-bathed (9), and the ethanol is volatilized to obtain a paste-like gingerol product (10 ), the concentrated solution (11) was dried (12) to obtain the powdered curcumin finished product (13); the centrifuged precipitate (6) was mixed with 0.05mol / L phosphate buffer (14) with a pH value of 6 to 8 (containing 5 ~10mmol / L EDTA and 15~20mmol / L L-Cysteine ​​enzyme protection agent) according to the weight of the precipitate and the volume of the buffer W:V=1:3~1:5 for reconstitution (15), the filtered supernatant centrifuge (16) at 3000-4000rpm for 5-10min, then slowly add 50%-70% (v / v) ethanol (17) and let stand in ice bath (18) take out after 5-8h, and suction filter ( 19), get w...

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Abstract

The invention relates to a method for combined extraction of a variety of functional components of ginger. The method comprises the following steps of: cutting the ginger into small pieces, adding anhydrous ethanol, pulping, centrifuging, performing rotary evaporation on supernatant fluid after centrifuging in vacuum, treating a rotary evaporation solution in a water bath to volatilize the ethanol, drying the solution to obtain gingerol, and drying a concentrated solution to obtain a powdery curcumin finished product; and re-dissolving sediment after centrifuging in a phosphate buffer solution, filtering, centrifuging the supernatant fluid, slowly adding the ethanol, performing ice bath on the supernatant fluid, standing, performing suction filtration to obtain wet enzyme powder, freezing and drying to obtain dry enzyme powder. By adopting the method, three types of active ingredients including the gingerol, curcumin and ginger protease can be obtained synchronously, so that the production efficiency is improved, the production cost is reduced, the utilization ratio of raw materials is improved, and the method is high in applicability and wide in application range and has a broad industrialization and market prospect.

Description

(1) Technical field [0001] The invention relates to a method for jointly extracting multiple functional components of ginger, which belongs to the fields of light industry and agriculture. (2) Background technology [0002] Modern medical research has confirmed that ginger has the functions of protecting the liver and gallbladder, invigorating the stomach and antiemetic, promoting blood circulation, regulating the central nervous system, anti-inflammatory, antibacterial, and insecticidal. Recent medical research has found that ginger has anti-coagulant, blood pressure-lowering, blood-fat-lowering, anti-aging, anti-tumor effects, and the research on its related physiologically active ingredients is also quite active. Among them, the most important physiologically active ingredients are ginger protease, gingerol, curcumin, etc., which have been proved by modern medicine to be the basis of various physiological and pharmacological effects of ginger, and can be used as the main ...

Claims

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Application Information

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IPC IPC(8): C07C49/255C07C45/81C07C45/78C12N9/50
Inventor 唐晓珍乔旭光高晓东
Owner SHANDONG AGRICULTURAL UNIVERSITY
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