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OGP-CTx fusion protein for treating osteoporosis and relieving pain and nucleic acid encoding same

A fusion protein and osteoporosis technology, applied in the field of fusion protein and nucleic acid encoding the fusion protein, can solve problems such as inconvenience of patients, achieve the effects of less psychological burden, eliminating pain, and long treatment process

Inactive Publication Date: 2014-06-11
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] If the patient takes multiple drugs to treat osteoporosis and relieve pain, drug antagonistic reactions are likely to occur; to stagger the time of taking drugs, on the one hand, the half-life of the drug must be considered, and on the other hand, the effective concentration of the drug must also be considered, which will cause inconvenience to the patient
[0005] There is currently no drug that can effectively treat osteoporosis and relieve pain at the same time

Method used

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  • OGP-CTx fusion protein for treating osteoporosis and relieving pain and nucleic acid encoding same
  • OGP-CTx fusion protein for treating osteoporosis and relieving pain and nucleic acid encoding same
  • OGP-CTx fusion protein for treating osteoporosis and relieving pain and nucleic acid encoding same

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Experimental program
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specific Embodiment approach 1

[0012] Specific embodiment 1: The amino acid sequence of the OGP-CTx fusion protein used for the treatment of osteoporosis and analgesia in this embodiment is shown in SEQ ID NO:2.

[0013] The nucleic acid sequence of the OGP-CTx fusion protein used in the treatment of osteoporosis and analgesia in this embodiment is shown in SEQ ID NO: 1. The nucleic acid sequence was synthesized by a bioengineering company to produce a plasmid vector pUC ( OGP / CTx). The pUC (OGP / CTx) vector was digested with BamH I and EcoRI. (The digestion reaction system is shown in Table 1. The digestion reaction was reacted at 37°C for 10 hours, then 1.5% agarose gel electrophoresis, the target fragment Purified and recovered with DNA GEL EXTRACTION KIT), and digested pGEX-6P-1 with BamHI and EcoRI (the digestion reaction system is shown in Table 2, and the digestion reaction was reacted at 37°C for 10 hours, and then 0.6% agarose gel Electrophoresis, the target fragment is purified and recovered with DNA...

specific Embodiment approach 2

[0060] Embodiment 2: The nucleic acid sequence of the OGP-CTx fusion protein used in the treatment of osteoporosis and analgesia in this embodiment is shown in SEQ ID NO:1.

[0061] The nucleic acid sequence of the OGP-CTx fusion protein used in the treatment of osteoporosis and analgesia in this embodiment is synthesized by a bioengineering company.

[0062] In this embodiment, the nucleic acid sequence of the OGP-CTx fusion protein used to treat osteoporosis and analgesia inserts a KpnI restriction site between OGP and CTx, which can not only improve the stability of the encoded fusion protein, but also change the fusion protein. Its secondary structure improves its biological performance.

[0063] In this embodiment, BamHI and EcoRI restriction sites are added on both sides of the fusion protein gene, and the coding base sequence of the fusion gene is redesigned according to the characteristics of E. coli preferred codons.

[0064]

[0065]

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Abstract

The invention provides an OGP-CTx fusion protein for treating osteoporosis and relieving pain and a nucleic acid encoding the same, relating to a fusion protein and a nucleic acid encoding the same and overcoming the defect that drugs for effectively treating osteoporosis and relieving pain at the same time do not exist at present. The amino acid sequence of the OGP-CTx fusion protein for treating osteoporosis and relieving pain is shown in SEQ ID NO:2. The sequence of the nucleic acid of the fusion protein is shown in SEQ ID NO:1. The fusion protein can be used in the field of drugs.

Description

Technical field [0001] The invention relates to a fusion protein and a nucleic acid encoding the fusion protein. Background technique [0002] The elderly have a higher incidence of osteoporosis. There are 200 million osteoporosis patients worldwide, and there are more women than men. According to the standards of the World Health Organization (WHO), the results of the National Health and Nutrition Survey of the United States (NHANES Ⅲ, 1988~1994) show that osteoporosis seriously affects the quality of life of the elderly. Among people over 50, 1 / 2 of women, One in five men will have osteoporotic fractures in their lifetime. Once a patient has experienced an osteoporotic fracture for the first time, the risk of secondary fractures is significantly increased. The elderly in China rank first in the world. There are 90 million patients with osteoporosis, accounting for 7.1% of the total population. With the aging of society, the incidence of osteoporosis is on the rise. It is expe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62A61P19/10A61P29/00
Inventor 余琼
Owner HEILONGJIANG UNIV