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Pasty biocontrol preparation for cucumber fusarium wilt, and preparation method, application and special strain thereof

A technology for cucumber fusarium wilt and biocontrol preparation, which is applied in the field of microorganisms to achieve the effects of good control effect, stable quality and large profit margins

Active Publication Date: 2015-05-13
JIANGSU GENGYUN CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there are few reports on genetically engineered bacterial strains that use fungi and their paste biocontrol agents to effectively control cucumber wilt

Method used

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  • Pasty biocontrol preparation for cucumber fusarium wilt, and preparation method, application and special strain thereof
  • Pasty biocontrol preparation for cucumber fusarium wilt, and preparation method, application and special strain thereof

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preparation example Construction

[0045] Specifically, the preparation method of the pasty biocontrol preparation prepared by the control cucumber wilt bacterial strain GY20 of the present invention comprises the following steps:

[0046] (A) Obtaining the bacterial solution

[0047] Use a sterile inoculation knife to pick out the GY20 spores that have been cultivated on the potato dextrose agar medium (PDA) in advance, and wash them with sterile water to adjust the spore content to 10. 5 The spore / mL inoculum was inoculated into a 750 mL Erlenmeyer flask containing 200 mL of potato grape liquid medium (PD) at 5% (V / V) inoculum volume, at 26 °C and 150 rpm / min Shake culture for 48 h, inoculate 200 mL aliquots of the culture solution obtained in the previous step into 4 Erlenmeyer flasks with a volume of 2000 mL filled with 500 mL PD culture solution, shake and culture at 150 rpm and 26 °C for 48 h; 2000mL of culture medium was inoculated into a 30L fermenter (Zhenjiang Dongfang GUS-30) containing 20L of cultu...

Embodiment 1

[0053] Embodiment 1 pasty biocontrol preparation and preparation method thereof

[0054] The components of the pasty biocontrol preparation are:

[0055] Active ingredient [Trichoderma aculeatus ( Trichoderma asperellum ) GY20 mycelium and chlamydospore mixture] 94.999%

[0056] Copper ion inhibitor MDA-5 0.001%

[0057] Potato starch 5%

[0058] pH 3

[0059] The percentages mentioned above are percentages by weight.

[0060] The preparation method of above-mentioned pasty biocontrol preparation comprises the following steps:

[0061] (A) Obtaining the bacterial solution

[0062] Use a sterile inoculation knife to pick out the GY20 spores that have been cultivated on the potato dextrose agar medium (PDA) in advance, and wash them with sterile water to adjust the spore content to 10. 5 The spore / mL inoculum was inoculated into a 750 mL Erlenmeyer flask containing 200 mL of potato grape liquid medium (PD) at 5% (V / V) inoculum volume, at 26 °C and 150 rpm / min Shake cult...

Embodiment 2-3

[0066] Prepare embodiment 2-3 according to the method for embodiment 1, difference is as shown in table 1:

[0067] Table 1 Example 2-3 preparation method

[0068] Components / Examples of Paste Biocontrol Preparations Example 2 Example 3 active ingredient 89.95% 91.99% Copper ion inhibitor MDA-5 0.05% 0.01% potato starch 10% 8% PH value 4.0 3.5

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Abstract

The invention relates to a pasty biocontrol preparation for cucumber fusarium wilt, and a preparation method, application and a special strain thereof. The classification designation of the special strain is Trichoderma asperellum GY20, and the collection number is CGMCC NO:4899. The pasty biocontrol preparation provided by the invention takes hyphae and chlamydospores of the Trichoderma asperellum GY20 as active components of the preparation. According to the invention, the pasty biocontrol preparation has the biggest advantage of stable product quality, and can be stored for 18 months at room temperature and 3 years under refrigeration conditions; the pasty biocontrol preparation has favorable control effect on cucumber fusarium wilt; and waste water, waste gas and waste residues are never generated, thereby being beneficial to environmental protection.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a paste-like biocontrol preparation for cucumber wilt, [0002] Preparation method, application and special bacterial strain thereof. Background technique [0003] Cucumber is a globally important vegetable crop. In recent years, due to the expansion of cucumber cultivation area year by year, coupled with the large-scale transport of vegetables from south to north, cucumber has become an important vegetable that can be supplied year-round, and occupies an important position in the "vegetable basket project". However, with the scale, base, specialization, and related changes in the ecological environment of cucumber production, the problem of diseases in cucumber production is very prominent, and the degree of occurrence is becoming more and more serious, especially soil-borne diseases such as cucumber wilt are more harmful , has seriously affected the yield and quality of melon ve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01N25/00A01P3/00C12R1/885
Inventor 李楠张海军罗志会刘慕兰李惠
Owner JIANGSU GENGYUN CHEM CO LTD