Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Procalcitonin detection test strip and preparation method thereof

A procalcitonin and detection test paper technology, which is applied in the biological field to achieve the effect of rapid detection means and sensitivity improvement

Active Publication Date: 2013-04-03
SHENZHEN BLOT BIOTECH
View PDF6 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Colloidal gold immunochromatography is fast and simple, but only semi-quantitative

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Procalcitonin detection test strip and preparation method thereof
  • Procalcitonin detection test strip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Preparation of conjugate release region

[0041] Add 2 μmol of quantum dots with a particle size of 5 nm, an excitation wavelength of 365 nm, and an emission wavelength of 605 nm and 1 mg of anti-human procalcitonin monoclonal antibody (purchased from Shenzhen Berrault Biological Products) into 1 mL of pH7.4 and 50 mM phosphate buffer solution. Co., Ltd.), shake gently at room temperature for 2 hours, then centrifuge at 12,000 r / min for 30 minutes, and take the precipitate to obtain the quantum dot-labeled procalcitonin monoclonal antibody.

[0042] After the quantum dot-labeled procalcitonin monoclonal antibody is treated with a stabilizer, it is evenly sprayed on the glass cellulose membrane at an amount of 65 μL per square centimeter, and freeze-dried to obtain the conjugate release area.

Embodiment 2

[0043] Embodiment 2: the preparation of reaction area

[0044] Spray 10 μL of 1 mg / mL procalcitonin on the nitrocellulose membrane in the reaction area, and freeze-dry to obtain the test area.

[0045] Spray 10 μL of 1 mg / mL anti-mouse IgG antibody (purchased from Shenzhen Berrault Biological Products Co., Ltd.) on the nitrocellulose membrane in the reaction area, and freeze-dry to obtain the control area.

Embodiment 3

[0046] Embodiment 3: the preparation of test strip

[0047] The sample application area, the conjugate release area obtained in Example 1, the reaction area obtained in Example 2, and the water absorption area are successively pasted on the backing to overlap each other.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Excitation wavelengthaaaaaaaaaa
Emission wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of biotechnology and discloses a procalcitonin detection test strip and a preparation method thereof. The procalcitonin detection test strip comprises a sample feeding region, a conjugate releasing region, a reaction region and a water absorbing region which are arranged in sequence; the conjugate releasing region comprises a conjugate releasing cushion of a procalcitonin monoclonal antibody covered by a quantum dot mark; the reaction region is divided into a testing region and a control region; and the reaction region is characterized in that the testing region is covered by procalcitonin and the control region is covered by a solid support of an anti-rat IgG antibody. The procalcitonin detection test strip disclosed by the invention takes a novel fluorescent dye quantum dot to replace traditional colloidal gold so as to accurately, quantitatively, rapidly and sensitively detect the procalcitonin in human blood serum; and a needed instrument is different from a large-sized dear instrument of a previous clinical laboratory, and only a small-sized fluorescent quantitative analyzer is needed to realize real-time detection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a procalcitonin detection test strip and a preparation method thereof. Background technique [0002] Procalcitonin (PCT), the precursor of calcitonin, is mainly synthesized in the parafollicular cells of the thyroid gland. It is a glycoprotein composed of 116 amino acids with a molecular weight of about 13KD. It can be gradually cleaved into amino PCT of 57 amino acids, CT of 32 amino acids and calcitonin of 21 amino acids under the action of enzymes. Procalcitonin is the product of expression of the calcitonin I gene (CAI C-I) on chromosome 11, and in the absence of infection, extrathyroidal CALC-I expression is suppressed and largely confined to the neuroendocrine cells of the thyroid and lung There is a certain degree of expression, and it can induce the expression of CALC-I and the continuous release of PCT in various types of cells in various tissues throughout the body during ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/577G01N33/533
Inventor 马伟民张永顶马新民
Owner SHENZHEN BLOT BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com