Method for degrading eupatorium adenophorum into fermentable reducing sugar

Abstract

The invention discloses a method for degrading eupatorium adenophorum into fermentable reducing sugar, relating to a method for turning the harmful eupatorium adenophorum into a benefit. The invention aims to provide a method for degrading straw celluloses into fermentable reducing sugar by using a smaller amount of diluted acid and cellulose enzyme. The method is characterized by comprising the following steps of: firstly, adding a buffer solution into the eupatorium adenophorum; then, adding 1-7ml of HC1 solution with the concentration of 0.005-0.10mol / L into every 50ml of buffer solution by taking the dosage of the buffer solution as a reference, and then, pre-treating for 5-180min to obtain treated reaction liquid; and finally, adding the cellulose enzyme into the treated reaction liquid, and then, degrading the eupatorium adenophorum. The reducing sugar treated by using the method can be directly used for fermenting and producing ethanol without any treatment.

Description

technical field [0001] The invention relates to a method for turning waste of Eupatorium adenophorum into treasure, in particular to a method for degrading Eupatoria adenophorum into fermentable reducing sugar. Background technique [0002] In view of the existence of problems such as insufficient petroleum resources, energy supply shortage, fuel safety and environmental pollution pressure, the development of new renewable raw materials and energy has become a trend. Fuel ethanol has the advantages of renewable, non-environmental pollution, and can be used as motor vehicle fuel. Highly valued by the government and society. However, traditional fuel ethanol production mainly uses starchy raw materials such as wheat, corn, and dried potatoes, or sugary raw materials such as sugarcane molasses and beet molasses, which require a large amount of crops and high production costs. [0003] Eupatorium adenophorum (Crofton Weed), perennial herb or subshrub. The "killer" in the plant...

AI Technical Summary

Problems solved by technology

It can be roughly divided into two types: one is that the lignocellulose is subjected to concentrated acid, concentrated alkali or dilute acid, dilute alkali, pretreated by high temperature, high pressure, microwave, ultrasonic and steam explosion, and then hydrolyzed or enzymatically hydrolyzed to restore Sugar, this method has the disadvantages of large amount of acid and alkali, low recovery rate, expensive recovery device, serious environmental pollution, high energy consumption, and poor operating process safety; the other is to directly degrade it into reducing sugar with cellulase preparations. This method has the disadvantages of large amount of cellulase, high cost, and low enzymatic hydrolysis efficiency.

In addition, the reducing sugar obtained by these traditional methods cannot be directly used in the fermentation production of ethanol, and the treatment liquid needs to be processed by precipitation, extraction and other methods before continuing the subsequent saccharification and fermentation process to produce ethanol

[0006] That is to say: there is no suitable process technology for reference in the prior art

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