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Application of cornuside in preparing pharmaceuticals used to cure diabetic nephropathy

A technology of corni schizoside and diabetic nephropathy, which is applied in the application field of corni schizoside in the preparation of drugs for the treatment of diabetic nephropathy, and can solve the problems that the protective effect of kidney injury has not yet been reported in relevant research reports

Inactive Publication Date: 2013-04-24
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies in the literature have shown that Cornus officinalis has good anti-inflammatory, anti-viral, hepatoprotective and cardioprotective effects, but there is no relevant research report on the protective effect of this effect on the complications of kidney damage caused by diabetes

Method used

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  • Application of cornuside in preparing pharmaceuticals used to cure diabetic nephropathy
  • Application of cornuside in preparing pharmaceuticals used to cure diabetic nephropathy
  • Application of cornuside in preparing pharmaceuticals used to cure diabetic nephropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Preparation of Cornus officinalis

[0026] 1. Experimental materials

[0027] Instruments: raw cornus, Agilent LC / MSD TOF (USA); Agilent 1100 binary pump (USA); Agilent 1100 standard autosampler (USA); Milli-Q ultrapure water machine (Millipore, Bedford, MA, USA )

[0028] Reagents: formic acid (analytical grade, Shanghai Lingfeng Chemical Reagent Co., Ltd.); acetonitrile (chromatographic grade, Fisher Science); water is ultrapure water.

[0029] Preparation of samples for methanol extraction

[0030] Weigh 1 g each of Cornus officinalis raw and finished product powder, weigh them accurately, place them in 25 mL volumetric flasks, add 80% methanol to make up to the mark, seal tightly, weigh, extract by ultrasonic for 45 min, weigh again, and supplement with methanol lost weight.

[0031] 2. Establishment of the experimental method

[0032] Liquid chromatography conditions: the chromatographic column is Agilent Zorbax C 18(250mm × 4. 6 mm, 5.0 μm) column,...

Embodiment 2

[0054] Embodiment 2 efficacy

[0055] 1. Experimental animals and models

[0056] Rat animal model of diabetic nephropathy induced by unilateral nephrectomy plus intraperitoneal injection of streptozotocin (STZ). Select 20 adult male SD rats, weighing about 260 g, and randomly divide them into normal group, model group and drug treatment group, with 10 rats in each group.

[0057] Rats with experimental diabetic nephropathy were established by a single intraperitoneal injection of streptozotocin (STZ, streptozotocin) for 8 weeks, and diabetes was formed in the first 4 weeks (blood glucose concentration was not lower than 16.7 mmol / L, and finally reached 25 mmol / L); The diabetic nephropathy model was established four weeks later.

[0058] 2. Administration method

[0059] On the next day after the successful replication of the diabetes model, the rats in the Cornus officinalis group were orally administered 0.2 g / kg of Cornus officinalis made from an ultra-pure aqueous solut...

Embodiment 3

[0075] Example 3 Capsules or tablets containing corni schizoside

[0076] Preparation prescription: 120g of cornel schizoside, 120g of microcrystalline fiber, 30g of starch, 28g of lactose, and 3g of dextrin.

[0077] Preparation method: Mix the components evenly according to the proportion according to the prescription, then add 80% ethanol solution to make granules, and make granules; take half of the capsules and make 800 tablets; the other half is compressed into tablets to make 800 tablets agent.

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PUM

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Abstract

The invention relates to the application of cornuside in preparing pharmaceuticals used for curing diabetic nephropathy. The invention raises a novel application of cornuside in preparing pharmaceuticals used to cure diabetic nephropathy and provides a novel pharmaceutical choice for curing diabetic nephropathy. Through experimental verification, 0.1g-5.5g / kg dosage of cornuside has remarkable effects in curing diabetic nephropathy. Cornuside has satisfying effects of anti-inflammatory damage and improving renal function on renal tissue under the condition of diabetes, and is effective pharmaceutical in curing diabetic nephropathy.

Description

technical field [0001] The invention relates to the application of corni schizoside in the preparation of medicine for treating diabetic nephropathy. Background technique [0002] Diabetic nephropathy (DN), also known as diabetic glomerulosclerosis, is a common chronic microvascular complication of diabetes, usually referring to glomerular basement membrane thickening, mesangial expansion and extracellular matrix hyperplasia caused by diabetes, It leads to glomerular hyperfiltration and proteinuria, which is characterized by damage and sclerosis of glomerular blood vessels, forming nodular lesions, and then abnormal renal function, eventually forming end-stage renal failure (ESRD). End-stage renal failure is one of the leading causes of death in DN patients. According to the statistics of the World Health Organization, in 2011, 336 million people worldwide suffered from diabetes. Diabetes has become the third killer in the world, second only to cardiovascular and cerebrova...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P13/12A61P3/10
Inventor 曹岗蔡宝昌杜伟锋岳显可
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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