Method for preparing bivalent yolk antibody for preventing and treating duck virus hepatitis I and III

A duck viral hepatitis, egg yolk antibody technology, applied in antiviral immunoglobulins, antiviral agents, egg-derived immunoglobulins, etc., can solve the problems of unsatisfactory treatment effect, low cure rate, etc. High potency, high purity effect

Inactive Publication Date: 2013-04-24
TIANJIN ZHONGSHENG TIAOZHAN BIOTECH
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0006] In recent years, DHV-I and DHV-III have been isolated simultaneously from sick ducks in my country, which proves that duck viral hepatitis in quite a few areas is a mixed infection of DHV-I and DHV-III, and the fatality rate of mixed infection is higher than that of any of them. However, the cure rate is lower than that of any single infection; and most of the existing duck viral hepatitis vaccines, hyperimmune serum or hyperimmune egg yolk antibodies are developed against type Ⅰ duck hepatitis virus, so for The curative effects of DHV-III single infection and DHV-Ⅰ and DHV-III mixed infection are not satisfactory, which brings new challenges to the prevention and treatment of duck viral hepatitis

Method used

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Embodiment

[0039] The preparation method of the bivalent egg yolk antibody for preventing and treating Type I and Type III duck viral hepatitis of the present invention comprises the following steps:

[0040] 1. Preparation of divalent high-free eggs with type Ⅰ and type Ⅲ duck viral hepatitis

[0041] (1) Select healthy high-yield laying hens for isolation breeding, strengthen feed nutrition, and feed special antibiotic preparations to prevent bacterial infection;

[0042] (2) Preparation of bivalent inactivated vaccines of type Ⅰ and type Ⅲ duck hepatitis virus

[0043] A. Preparation of water phase

[0044] Dilution with cryopreserved type Ⅰ and type Ⅲ duck viral hepatitis strains were used to inoculate 10-day-old duck embryos respectively. After 24-72 hours, aseptically collected dead duck embryo bodies and embryo fluid, homogenized, centrifuged after freezing and thawing, Take the supernatant, mix equal volumes to make a virus liquid and inactivate it, take the inactivated virus l...

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Abstract

The invention discloses a method for preparing a bivalent yolk antibody for preventing and treating duck virus hepatitis I and III. The bivalent yolk antibody can be used for effectively preventing and treating the duck virus hepatitis I and III single and mixed infection and solving the problems that the existing vaccines, serum antibodies and yolk antibodies are not effective to the mixed infection. In addition, the bivalent yolk antibody is extracted by adopting a caprylic acid precipitation method. The method is safe, low in cost and suitable for mass production. The prepared bivalent yolk antibody has high purity and high titer, is stable in nature and is easy for storage.

Description

technical field [0001] The invention relates to the technical field of biological products for poultry, in particular to a preparation method of a bivalent egg yolk antibody for preventing and treating type I and type III duck viral hepatitis. Background technique [0002] Duck virus hepatitis (DVH) is a highly fatal and acute infectious disease of ducklings caused by duck hepatitis virus (DHV). Duck hepatitis virus (DHV) includes 4 viruses of 3 virus families, Hepadnaviridae, Astroviridae and Picornaviridae. The duck hepatitis virus of the Hepadnaviridae causes duck hepatitis B, which has no significant pathogenicity to ducks; the Astroviridae causes type II duck hepatitis, which has only been reported in the UK so far; the Picornaviridae mainly cause Type I type and type III duck hepatitis. [0003] The clinical symptoms and pathological changes of type Ⅰ and type Ⅲ duck hepatitis are similar, but the neutralization test and fluorescent antibody test prove that the two h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C07K16/02A61P31/14A61P1/16
Inventor 宋秀梅王建刘丽丽殷立伟葛兆宁王震
Owner TIANJIN ZHONGSHENG TIAOZHAN BIOTECH
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