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Gloconacetobacter xylinum and method for fermenting high-yield bacterial celluloses through mixed flora

A technology of bacterial cellulose and Acetobacter xylinum, applied in the direction of microorganism-based methods, bacteria, fermentation, etc., can solve the problems of long fermentation cycle, low production efficiency, and large-scale production restrictions, and achieve dense and uniform texture, simplify Labor intensity and the effect of shortening production time

Active Publication Date: 2013-04-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing bacterial cellulose production technology mainly has the following problems: (1) The fermentation cycle is long, up to 7-12 days; (2) The shallow plate fermentation is used, and the growth and reproduction of bacteria and the production of cellulose produced by fermentation are not carried out separately , resulting in long fermentation cycle, low production efficiency, and limited large-scale production; (3) The medium for fermentation and production of bacterial cellulose is not suitable, and the production formula is not designed according to the growth needs of Acetobacter xylinum itself, resulting in low production efficiency
Above-mentioned prior art all does not relate to the mixed cultivation of Acetobacter xylinum, Lactobacillus and acetic acid bacteria

Method used

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  • Gloconacetobacter xylinum and method for fermenting high-yield bacterial celluloses through mixed flora
  • Gloconacetobacter xylinum and method for fermenting high-yield bacterial celluloses through mixed flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] In the first step, Acetobacter AC-0720, Lactobacillus DMDL9010 and Acetobacter xylinum HNX01 were respectively cultured at high density according to the following steps, and the number of live bacteria in the seed liquid of the three strains reached 10 respectively. 8 Above CFU / mL:

[0042] 1. Inoculate 0.1g of Lactobacillus DMDL9010 freeze-dried powder into 10mL of DMDL9010 seed medium, and culture it statically at 25°C for 20 hours to make the first seed liquid of DMDL9010; inoculate the first seed liquid with a volume ratio of 5:100 Into the DMDL9010 seed medium, cultured statically at 25°C for 28 hours to prepare the second seed solution of DMDL9010.

[0043] The formula of described Lactobacillus DMDL9010 seed medium is: peptone 8.0g / L, yeast powder 8g / L, glucose 25g / L, triamine citrate 1.0g / L, MgSO 4 .7H 2 O0.3g / L, beef extract 12.5g / L, KH 2 PO 4 2.0g / L, MnSO 4 .4H 2 0.04g / L, sodium acetate 2.0g / L, Tween 801.1g / L; The preparation method of described DMDL901...

Embodiment 2

[0054] In the first step, Acetobacter AC-0720, Lactobacillus DMDL9010 and Acetobacter xylinum HNX01 were cultured at high density according to the following steps, and the number of viable bacteria in the seed liquid of the three strains reached 10 respectively. 8 CFU / mL or more.

[0055] 1. Inoculate 0.5g of Lactobacillus DMDL9010 freeze-dried powder into 10mL of DMDL9010 seed medium, and culture it statically at 30°C for 24 hours to make the first seed liquid of DMDL9010; inoculate the first seed liquid with a volume ratio of 10:100 Into the DMDL9010 seed medium, cultured statically at 30°C for 24 hours to prepare the second seed solution of DMDL9010.

[0056] The formula of described Lactobacillus DMDL9010 seed medium is: peptone 12.0g / L, yeast powder 5g / L, glucose 15g / L, triamine citrate 1.5g / L, MgSO 4 .7H 2 O0.1g / L, beef extract 15g / L, KH 2 PO 4 0.5g / L, MnSO 4 .4H 2 0.09g / L, sodium acetate 3.5g / L, Tween 800.5g / L; The preparation method of described DMDL9010 seed cu...

Embodiment 3

[0066] In the first step, Acetobacter AC-0720, Lactobacillus DMDL9010 and Acetobacter xylinum HNX01 were respectively cultured at high density according to the following steps, and the number of live bacteria in the seed liquid of the three strains reached 10 respectively. 8 More than CFU / ml.

[0067] 1. Inoculate 0.3g of Lactobacillus DMDL9010 freeze-dried powder into 10mL of DMDL9010 seed medium, and culture it statically at 28°C for 26 hours to make the first seed liquid of DMDL9010; inoculate the first seed liquid with a volume ratio of 6:100 Into the DMDL9010 seed medium, and cultured statically at 28°C for 26 hours to prepare the second seed solution of DMDL9010.

[0068] The formula of described Lactobacillus DMDL9010 seed medium is: peptone 10.0g / L, yeast powder 2g / L, glucose 23g / L, triamine citrate 3.0g / L, MgSO 4 .7H 2 O0.2g / L, beef extract 5.0g / L, KH 2 PO 4 3.5g / L, MnSO 4 .4H 2 0.01g / L, sodium acetate 0.5g / L, Tween 800.7g / L; The preparation method of described...

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Abstract

The invention discloses gloconacetobacter xylinum and a method for fermenting high-yield bacterial celluloses through mixed flora. The gloconacetobacter xylinum HNX01 with the preservation number of CGMCC No. 5173 was preserved in the CGMCC (China General Microbiological Culture Collection Center) on Aug. 19, 2011. According to the method for fermenting high-yield bacterial celluloses through the mixed flora, the mixed flora includes gloconacetobacter xylinum CGMCC No. 5173, acetobacter sp. CGMCC No. 6641 and lactobacillus sp. CGMCC No. 5172. The yield of the obtained bacterial celluloses is more than 26.5 g / L (dry basis weight). The method provides sufficient nutrition for the growth of the gloconacetobacter xylinum and fully utilizes a culture medium to ferment high-yield bacterial celluloses with compact and uniform structures.

Description

technical field [0001] The invention relates to the production of bacterial cellulose, in particular to a method for fermenting high-yield bacterial cellulose by Acetobacter xylinum and mixed flora. Background technique [0002] "Coconut fruit" is a kind of gelatinous thick film produced by microbial fermentation with coconut water as the main raw material. It is also called "coconut fiber fruit" in the agricultural industry standard NY / T1522-2007. "Nacco" has been used as a food for more than 70 years, because most of the microorganisms that produce "coconut" are mainly bacteria, such as Acetobacter xylinum (Gloconacetobacter xylinum), Acetobaceria aceti (Acetobaceria aceti), producing Acetobacter acotigenum and Acetobacter pastcurianum, and Acetobacter xylinum has the strongest synthetic ability. Furthermore, since the essence of "coconut" is a kind of cellulose with high purity, "coconut" can also be called bacterial cellulose (Bacterial Cellulose). According to the diff...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P39/00C12P19/04C12R1/02C12R1/225C12R1/01
Inventor 刘冬梅王盼费永涛黎嘉惠黄丹华吴晖唐语谦余以刚
Owner SOUTH CHINA UNIV OF TECH
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