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Kit for determination of apolipoprotein C2 by using immunoturbidimetry

A technology of apolipoprotein and immunoturbidimetry, which is applied in the direction of biological testing, material inspection products, etc., can solve the problems of time-consuming, cumbersome measurement process, batch-to-batch difference and high repeatability, and achieve simplified operation methods and steps, high The effect of clinical application value and detection efficiency improvement

Active Publication Date: 2013-04-24
潍坊三维生物工程集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many deficiencies in the existing APOC2 detection technology: for example, special equipment is required, samples need to be pretreated, and automatic biochemical analyzers cannot be used for batch detection and analysis, etc.
This method is a heterogeneous immunoassay system, and the determination process is cumbersome, time-consuming, and lacks unit test running time; the degree of automation is not high, and the inter-batch difference and repeatability are relatively large; it needs to be equipped with a variety of special equipment, which increases to a certain extent. cost

Method used

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  • Kit for determination of apolipoprotein C2 by using immunoturbidimetry
  • Kit for determination of apolipoprotein C2 by using immunoturbidimetry
  • Kit for determination of apolipoprotein C2 by using immunoturbidimetry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Preparation of reagents:

[0037] Reagent R1:

[0038]

[0039] Reagent R2:

[0040] Rabbit anti-human APOC2 polyclonal antibody 15% (w / v)

[0041] Glycine (buffer) 500mM

[0042] Potassium chloride (electrolyte) 50g / L

[0043] Ethyl p-hydroxybenzoate (preservative) 1g / L

[0044] Calibrator:

[0045] Phosphate (buffer) 500mM

[0046] Disodium EDTA (stabilizer) 50g / L

[0047] Sodium azide (preservative) 1g / L

[0048] Butylated hydroxyanisole (antioxidant) 0.01g / L

[0049]According to the required concentration of the calibrator, a corresponding amount of APOC2 antigen is added to the solution to prepare the APOC2 calibrator. The calibrator can be a high-concentration single-point calibrator, which is diluted with normal saline to form 5 reference calibrators with different concentrations, or can be directly prepared into 5 reference calibrators with different concentrations. In this example, 5 reference calibration products with different concentrations w...

Embodiment 2

[0051] Reagent R1:

[0052]

[0053] Reagent R2:

[0054] Chicken anti-human APOC2 monoclonal antibody 30% (w / v)

[0055] Phosphate (buffer) 10mM

[0056] Sodium chloride (electrolyte) 5g / L

[0057] Sodium azide (preservative) 10g / L

[0058] Calibrator:

[0059]

[0060] In this embodiment, a high-concentration single-point reference calibrator was selected, and the APOC2 antigen concentration was 60 mg / L, and then sterilized by filtration with a 0.22 μm filter membrane, and stored at 2-8°C. When used, it is diluted with normal saline to form 5 reference calibration products with different concentrations, which are 0.1mg / L, 7.5mg / L, 15mg / L, 30mg / L, and 60mg / L.

Embodiment 3

[0062] 1. Preparation of reagents:

[0063] Reagent R1:

[0064]

[0065] Reagent R2:

[0066] Goat anti-human APOC2 polyclonal antibody 22% (w / v)

[0067] Phosphate (buffer) 60mM

[0068] Sodium chloride (electrolyte) 8.5g / L

[0069] Sodium azide (preservative) 2g / L

[0070] Calibrator:

[0071] Glycine (buffer) 20mM

[0072] Disodium EDTA (stabilizer) 8g / L

[0073] Sodium azide (preservative) 4g / L

[0074] Butylated hydroxyanisole (antioxidant) 0.4g / L

[0075] According to the required concentration of the calibrator, a corresponding amount of APOC2 antigen is added to the solution to prepare the APOC2 calibrator. The calibrator can be a high-concentration single-point calibrator, which is diluted with normal saline to form 5 reference calibrators with different concentrations, or can be directly prepared into 5 reference calibrators with different concentrations. In this example, 5 reference calibration products with different concentrations were prepared, name...

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Abstract

The invention provides a kit for determination of apolipoprotein C2 by using immunoturbidimetry. A reagent R1 contains 10 to 500 mM of a buffer solution, 1 to 100 g / L of a surfactant, 5 to 50 g / L of an electrolyte, 2 to 100 g / L of a high-molecular accelerator accelerator, 1 to 50 g / L of a stabilizing agent and 1 to 10 g / L of an antiseptic; a reagent R2 comprises 10 to 500 mM of the buffer solution, 150 to 300 g / L of an anti-human APOC2 antibody, 5 to 50 g / L of the electrolyte, and 1 to 10 g / L of the antiseptic; and a calibrator comprises 10 to 500 mM of the buffer solution, 0 to 100 g / L of an APOC2 antigen, 1 to 50 g / L of the stabilizing agent, 1 to 10 g / L of the antiseptic and 0.01 to 10 g / L of an anti-oxidant. The kit provided by the invention is easy and fast to use, can satisfy clinical requirements for rapid high flux detection of a sample and has the advantages of obviously improved detection efficiency, small batch difference and stable reagents.

Description

technical field [0001] The invention relates to the field of medical immunological in vitro diagnostic reagents, in particular to a kit for measuring apolipoprotein C2 (APOC2) by immunoturbidimetric method. Background technique [0002] Human Apo CII is a single-chain polypeptide containing 79 amino acid residues with a molecular weight of 9.1kD. The first synthesized Apo CII contains 101 amino acid residues, of which 22 amino acids constitute the signal peptide, which is converted into mature Apo CII after the signal peptide is removed. The Chuo-Fasman analysis of the amino acid sequence of Apo CII showed that the 13-22, 29-40, and 43-52 amino acid residues of Apo CII are amphipathic α-helices, which have the function of binding to lipids, and the 9-12, 23-26, 53-56 amino acid residues are β-turn structure, 61-74 AA residues are α-amphisexual helix, which is the region binding to lipids, 1-39, 54-69 amino acid residues are β- Corner structure. Studies on the hydrolyzed f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 宿明明王庆国张洋王爱龙
Owner 潍坊三维生物工程集团有限公司
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