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Trichoderma theobromicola and application thereof in prevention and treatment of vegetable diseases

A technology of Trichoderma cocoa and Trichoderma cocoa, applied in the direction of application, microbe-based methods, chemicals for biological control, etc., can solve the problems of destroying agricultural ecosystems, environmental pollution, etc., achieve environmental friendliness, and inhibit drug resistance and resistance development, improved efficacy in controlling fungal diseases

Active Publication Date: 2013-05-01
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, chemical agents are mostly used for prevention and control in production, but long-term and large-scale use of pesticides causes pathogenic bacteria to develop resistance, and seriously damages agricultural ecosystems, causing environmental pollution

Method used

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  • Trichoderma theobromicola and application thereof in prevention and treatment of vegetable diseases
  • Trichoderma theobromicola and application thereof in prevention and treatment of vegetable diseases
  • Trichoderma theobromicola and application thereof in prevention and treatment of vegetable diseases

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Experimental program
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Effect test

Embodiment 1

[0047] Embodiment 1, acquisition and preservation of cocoa Trichoderma (Trichoderma theobromicola) Gu11

[0048] 1. Sampling

[0049] Soil samples were collected from Beijing, Hebei, Xinjiang, Shandong, Hubei, etc.

[0050] 2. Isolation of strains

[0051] Strains were isolated by the coating method.

[0052] Weigh 10g of each soil sample, pour it into a sterile 500ml Erlenmeyer flask with a little Tween 80, glass beads and 100ml of distilled water, shake well, label, and shake for 30 minutes with a shaker. Then use a micro-syringe to suck 5ml of the soil suspension into a 250ml Erlenmeyer flask containing 45ml of sterilized distilled water, shake well, then suck 1ml from the 250ml Erlenmeyer flask and add it to a test tube containing 9ml of sterilized distilled water for doubling dilution , the sample was serially diluted to 10 -4 and 10 -5 Take 0.1ml of each sample and drop it on a PDA plate, spread it, and incubate it at 28°C for 48-72 hours, pick out the colony that p...

Embodiment 2

[0076] Embodiment 2, the application of cocoa Trichoderma Gu11 in the prevention and treatment of vegetable diseases

[0077] 1. Drugs for testing:

[0078] 1.1 Cocoa Trichoderma Gu11 inoculant

[0079]The preparation method of Trichoderma cocoa Gu11 inoculum is as follows: Inoculate Trichoderma cocoa Gu11 on a PDA medium plate, cultivate for about 5 days to grow spores, brush the spores with a brush, and use sterile water to make a concentration of 10 7 cfu / ml bacterial suspension, the bacterial suspension is Trichoderma cocoa Gu11 inoculum.

[0080] 1.2 Jinggangmycin solution

[0081] The preparation method of Jinggangmycin solution is as follows: 0.01 g of 3% Jinggangmycin aqueous solution (Tonglu Huifeng Biochemical Co., Ltd., Zhejiang Province) was dissolved in 20 ml of water to obtain Jinggangmycin solution.

[0082] 1.3 Dimethomorph solution

[0083] The preparation method of dimethomorph solution is as follows: 0.01 g of 50% dimethomorph (Hebei Shangrui Chemical Co...

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Abstract

The invention discloses a trichoderma theobromicola and an application of the trichoderma theobromicola in prevention and treatment of vegetable diseases. According to the invention, the strain number of the trichoderma theobromicola is Gu11; and the registration number of the trichoderma theobromicola in common microbe center of China Committee for Culture Collection of Microorganisms is CGMCC No. 6994. The trichoderma theobromicola and the preparation thereof disclosed by the invention are environment friendly, can suppress drug resistance developments of disease pathogens, is beneficial for promoting green goods and organic agriculture, has the advantages of low cost and no pollution and is safe for vegetable crops. As antagonistic microbes are introduced into the soil, fungal diseases can be effectively prevented and treated through biological control and the environment can be effectively improved; the balance of an agricultural ecological system is maintained; and a powerful support is provided for taking a sustainable development path.

Description

technical field [0001] The invention relates to a cocoa trichoderma and its application in preventing and treating vegetable diseases. Background technique [0002] At present, my country's vegetable planting area has exceeded 150 million mu, of which greenhouse vegetables and protected horticultural plants have exceeded 20.1 million mu. Due to the expansion of vegetable greenhouse cultivation area and continuous cropping, favorable ecological conditions are provided for the occurrence of diseases and insect pests. Vegetable wilt, gray mold, blight and other fungal diseases commonly occur in vegetable planting. When serious, they will cause a substantial reduction in yield, which is a thorny problem that farmers often encounter in the process of seedling cultivation and planting. [0003] Pepper blight is a soil-borne disease caused by Phytophthora capsici Leonian. It is a devastating disease of green peppers and can occur throughout the growth period of green peppers. Phy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 顾金刚李世贵刘好宝阮志勇张晓霞马晓彤张瑞颍
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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