Mixotrophic scenedesmus and application thereof in sewage resource treatment
A mixed nutrition and scenedesmus technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, unicellular algae, etc., can solve the limitation of commercial application of microalgae culture technology, weak anti-pollution ability, difficult to stabilize Survival and other issues to achieve the effect of reducing the risk of recycling
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Embodiment 1
[0018] Cultivating Scenedesmus sp. ZTY1 for a long time by intermittent culture can effectively increase the oil output per unit of sewage. Scenedesmus sp.ZTY1 was cultured intermittently with actual domestic sewage, and the concentration of dissolved organic carbon (DOC) in the sewage was 112mg·L -1 , the concentration of total nitrogen (TN) is 41.0mg·L -1 , the concentration of total phosphorus (TP) is 8.4mg·L -1 , the initial inoculation density was about 7.5×10 5 pcs mL -1 , under the conditions of light-to-dark ratio 14h:10h, temperature 25°C, and light intensity 800-1300lux, Scenedesmus sp. ZTY1 entered a stable period after 21 days of culture, and the concentration of carbon, nitrogen, and phosphorus obtained after centrifugation harvested Scenedesmus sp. (TN: 2.9mg·L -1 , TP:0.07mg·L -1 ), the removal rates of nitrogen and phosphorus in the primary effluent of urban sewage by this strain of Scenedesmus sp. were 92.9% and 99.1%, respectively.
[0019] When domesti...
Embodiment 2
[0021] The high-density cultivation of Scenedesmus ZTY1 in the way of sequencing batch culture can effectively reduce the time of sewage treatment and improve the treatment efficiency. Use actual domestic sewage (DOC is 72.9, TN is 50.4mg·L -1 , TP is 3.5mg·L -1 ) Sequenced batch culture of Scenedesmus sp.ZTY1 under the following conditions: light intensity 800-1300 lux, light-to-dark ratio 14h:10h, temperature 25°C, stirring rate 150r / min. The initial inoculation density of Scenedesmus sp. ZTY1 was 1.28×10 6 pcs mL -1 , after the end of each treatment cycle, centrifugal drainage, harvest 50% of the algae cells for the production of biodiesel, the remaining 50% of the algae cells remain in the system, add new domestic sewage, and continue the next cycle of cultivation process. The change of algae density and the removal effect on pollutants of Scenedesmus sp.ZTY1 during batch culture are as follows: figure 2 As shown, when the hydraulic retention time is set to three days...
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