Mixotrophic scenedesmus and application thereof in sewage resource treatment

A mixed nutrition and scenedesmus technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, unicellular algae, etc., can solve the limitation of commercial application of microalgae culture technology, weak anti-pollution ability, difficult to stabilize Survival and other issues to achieve the effect of reducing the risk of recycling

Active Publication Date: 2013-05-08
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common high-yielding algae species have weak anti-pollution ability, and it is difficult to survive stably in the actual sewage

Method used

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  • Mixotrophic scenedesmus and application thereof in sewage resource treatment
  • Mixotrophic scenedesmus and application thereof in sewage resource treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Cultivating Scenedesmus sp. ZTY1 for a long time by intermittent culture can effectively increase the oil output per unit of sewage. Scenedesmus sp.ZTY1 was cultured intermittently with actual domestic sewage, and the concentration of dissolved organic carbon (DOC) in the sewage was 112mg·L -1 , the concentration of total nitrogen (TN) is 41.0mg·L -1 , the concentration of total phosphorus (TP) is 8.4mg·L -1 , the initial inoculation density was about 7.5×10 5 pcs mL -1 , under the conditions of light-to-dark ratio 14h:10h, temperature 25°C, and light intensity 800-1300lux, Scenedesmus sp. ZTY1 entered a stable period after 21 days of culture, and the concentration of carbon, nitrogen, and phosphorus obtained after centrifugation harvested Scenedesmus sp. (TN: 2.9mg·L -1 , TP:0.07mg·L -1 ), the removal rates of nitrogen and phosphorus in the primary effluent of urban sewage by this strain of Scenedesmus sp. were 92.9% and 99.1%, respectively.

[0019] When domesti...

Embodiment 2

[0021] The high-density cultivation of Scenedesmus ZTY1 in the way of sequencing batch culture can effectively reduce the time of sewage treatment and improve the treatment efficiency. Use actual domestic sewage (DOC is 72.9, TN is 50.4mg·L -1 , TP is 3.5mg·L -1 ) Sequenced batch culture of Scenedesmus sp.ZTY1 under the following conditions: light intensity 800-1300 lux, light-to-dark ratio 14h:10h, temperature 25°C, stirring rate 150r / min. The initial inoculation density of Scenedesmus sp. ZTY1 was 1.28×10 6 pcs mL -1 , after the end of each treatment cycle, centrifugal drainage, harvest 50% of the algae cells for the production of biodiesel, the remaining 50% of the algae cells remain in the system, add new domestic sewage, and continue the next cycle of cultivation process. The change of algae density and the removal effect on pollutants of Scenedesmus sp.ZTY1 during batch culture are as follows: figure 2 As shown, when the hydraulic retention time is set to three days...

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Abstract

The invention relates to mixotrophic scenedesmus and an application in sewage resource treatment. The scenedesmus has strong anti-pollution capability and can grow stably in actual sewage (non-sterile), the heterotrophic capability is strong, the productivity of biomass and grease is high, namely organic matters in the sewage can be transformed to grease, nutrient substances in the sewage can be further utilized for absorbing carbon dioxide in atmosphere, nitrogen and phosphorus in the sewage can be removed in a sustained manner under the situation that an organic carbon source is used up, and when the sewage is taken as a matrix for inoculating the scenedesmus, the water quality can be purified, and the grease can be simultaneously accumulated, so that sewage resources can be effectively utilized, the waste can be changed to valuable things, and the mixotrophic scenedesmus further has the advantages of low operation cost, good quality of outlet water, simple and convenient technology, easiness in popularization and the like.

Description

technical field [0001] The invention relates to the fields of water pollution control and biodiesel production, in particular to a mixed trophic scenedesmus and its application in sewage resource treatment. Background technique [0002] In the field of water pollution control, the conventional treatment process represented by the activated sludge method is difficult to realize the resource utilization of sewage. The organic matter and inorganic salt in the sewage are lost in the form of gas or sludge along with the treatment of sewage, which aggravates the shortage of resources. status quo. In the field of biodiesel production, the high cost of microalgae cultivation is the main limiting factor restricting the commercialization of microalgae biodiesel. The cultivation of microalgae with sewage as a substrate can provide cheap biomass raw materials for the production of biodiesel and other biomass products. Thereby greatly reducing production costs. The common high-yielding...

Claims

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Application Information

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IPC IPC(8): C12N1/12C02F3/32C12R1/89
Inventor 胡洪营张天元
Owner TSINGHUA UNIV
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