Method for emptying solvent from liquid chromatography and gas chromatography on-line coupling interface

Abstract

The invention discloses a method for emptying a solvent from a liquid chromatography and gas chromatography on-line coupling interface. A solvent emptying device is connected between a pre-column of a liquid chromatograph and a gas chromatographic column; and the solvent emptying device comprises two three-way connectors and a two-direction changeover valve which is connected between the two three-way connectors. The solvent emptying device is connected between a reserving pre-column of the liquid chromatograph and a gas chromatographic separation column, and due to isolation of the reserving pre-column and the gas chromatographic separation column, a solvent almost can not enter the gas chromatographic separation column and a detector, so that the influence of a great quantity of liquid chromatographic mobile phases on a gas chromatographic detector (particularly an MS vacuum system) is avoided; and the volume of the introduced liquid chromatographic mobile phases is not limited, and high-boiling-point components can be prevented from entering an analytical column, so that the pollution to a chromatographic column system caused by a on-column sampling way is reduced, and the purification process of the reserving pre-column is accelerated.

Description

technical field [0001] The invention relates to a solvent evacuation method for an online liquid chromatography-gas chromatography interface, and belongs to the technical field of chromatography detection. Background technique [0002] Liquid chromatography-gas chromatography technology is a new multi-dimensional chromatography technology, which has both the high selectivity of liquid chromatography (LG) and the high efficiency and high sensitivity of gas chromatography (GC). It has strong processing, enrichment, separation and detection capabilities, and is of great significance to the analysis of complex organic mixtures such as environmental monitoring, pesticide testing, food testing, natural products, petrochemical and biomedicine, and samples with complex backgrounds. . [0003] In the study of complex systems, LC is often used to pre-separate the mixture into multiple fractions. For the target fractions of interest, GC / MS or selective detectors are used for further a...

AI Technical Summary

Problems solved by technology

The commonly used method of solvent evacuation is to discharge the solvent into the atmosphere through the tee joint, but this technology still has a small amount of solvent entering the analytical column and detector, especially for the mass spectrometer, because of its vacuum effect, there will be a large amount of solvent It is sucked into the analytical column and mass spectrometer system, thereby contaminating the ion source and causing the vacuum degree of the mass spectrometer to drop. In severe cases, it will cause the mass spectrometer to alarm or even shut down

Therefore, how to completely remove the solvent and introduce the components to be measured into the GC, there is no perfect solvent evacuation technology at present

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