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Efficient phosphate solubilizing growth-promoting bacteria and microbial agent prepared from same and application

A technology of microbial inoculants and composite microbial inoculants, applied in the directions of microorganisms, biocides, and microorganism-based methods, can solve the problems of scarcity of phosphorus-dissolving microbial strain resources and no reports of phosphorus-dissolving microbial inoculants, etc. The effect of efficiently dissolving insoluble phosphorus, improving the utilization rate of phosphorus fertilizer and saving chemical fertilizer resources

Inactive Publication Date: 2013-05-15
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Widely adaptable (soil, crop) phosphorus-dissolving microbial strain resources are scarce worldwide, and there are no reports on widely adaptable phosphorus-dissolving microbial agents

Method used

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  • Efficient phosphate solubilizing growth-promoting bacteria and microbial agent prepared from same and application
  • Efficient phosphate solubilizing growth-promoting bacteria and microbial agent prepared from same and application
  • Efficient phosphate solubilizing growth-promoting bacteria and microbial agent prepared from same and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The isolation of embodiment 1 pantoea pineapple P21 bacterial strain, cultivation and the preparation of phosphate-dissolving bacteria agent P21

[0056] Pantoea pineapple P21 was isolated from the soil of Kunyang RP (Kunyang RP) phosphate mining area in Yunnan, my country, and has been preserved in the General Microbiology Center of China Microbial Culture Collection Management Committee, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing Institute of Microbiology, Chinese Academy of Sciences, deposit date: February 9, 2012, deposit number CGMCC NO.5749.

[0057] Cultivation of Phosphorus Solubilizing and Growth-promoting Pantoea pineapple P21 and Preparation of Phosphorus Solubilizing Bacteria P21:

[0058] (1) Medium for Pantoea pineapple P21

[0059] Fermentation broth formula (kg / t fermentation broth): dipotassium hydrogen phosphate 0.1, potassium dihydrogen phosphate 0.1, sodium chloride 4.3, magnesium sulfate 0.1, starch 8.6, beef extract 4...

Embodiment 2

[0062] Embodiment 2 Preparation of efficient phosphorus-dissolving, disease-resistant, and production-increasing compound microbial bacterial agents

[0063] 1. Cultivation of Mortierella Minnesota MYA-31

[0064] (1) Composition of medium (kg / t fermentation broth)

[0065] Soluble starch 10kg, soybean cake powder 10kg, corn flour 5kg, glucose 1kg, sucrose 10kg, yeast powder 5kg, (NH 4 ) 2 SO 4 3kg, K 2 HPO 4 0.2kg, NaCl2.5kg, MgSO 4 ·7H 2 O0.1kg, CaCO 3 0.5kg, FeSO 4 0.001kg, boric acid (1%) 1L, pH7.0, prepared with water.

[0066] (2) Fermentation and cultivation process

[0067]Inoculate Mortierella minnesota MYA-31 on potato sucrose (PDA) medium and culture at 25-28°C for 3 days. Put the spores of Mortierella minnesota into a 1000mL Erlenmeyer flask, and culture in liquid at 26-28°C and 220r / min for 36h. Then put it into a 50L seed tank according to 1v / v% inoculum amount, at 200r / min, pH7.0, ventilation rate 0.7vvm, after culturing for 36h, then put it into a ...

Embodiment 3

[0072] Phosphorus dissolving effect under the condition of embodiment 3 Pantoea pineapple P21 culture dish

[0073] 1. Phosphorus-solubilizing strains P. pineapple P21 was isolated and screened from plant rhizosphere soil samples in the Yang Phosphate Mining Area of ​​Yunkun, China. At the same time, phosphorus-solubilizing strains P40 and P40-2 were isolated and screened from soil samples from other areas. Under the conditions of indoor solid culture dish and liquid culture medium, their phosphorus-dissolving ability was determined.

[0074] The ratio of the phosphorus-dissolving circle to the colony diameter of the phosphorus-dissolving strain P21 reached 5.43, and the water-soluble soluble (P 2 o 5 ) is 4.25mg; the phosphorus-dissolving circle of bacterial strain P40 and P40-2 is 4.00 and 1.56 with the colony diameter ratio respectively, and the phosphorus-dissolving amount in the phosphorus-dissolving circle (P 2 o 5 ) were 3.13 and 1.22 mg, respectively, and the phosp...

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Abstract

The invention provides efficient phosphate solubilizing growth-promoting bacteria and a microbial agent prepared from the same. The active ingredients of the microbial agent are pantoea ananatis P21 and hirsutella minnesotensis. According to the invention, the widely-applicable efficient phosphate solubilizing bacteria pantoea ananatis P21 and fungi for efficiently preventing nematodiasis are organically combined; and by utilizing the composite agent provided by the invention, the soil phosphorus is efficiently used, the utilization rate of phosphate fertilizer is improved, the function of microorganism in preventing nematodiasis is sufficiently realized, and an effect of increasing yield by solubilizing phosphate and preventing diseases is realized, thereby having great significance to sustainably increasing grain yield of the country, saving phosphate fertilizer resource, reducing nematodiasis and protecting the ecological environment of farmland.

Description

technical field [0001] The invention belongs to the field of microbial inoculum, in particular, relates to a high-efficiency phosphorus-dissolving and growth-promoting bacterium, a microbial inoculum prepared therefrom and its application. Background technique [0002] (1) Development Status of Phosphorus-Solubilizing Microbial Agents [0003] More than 90% of the arable land in my country is deficient in phosphorus. The use of phosphorus fertilizer is the main measure to solve crop yield increase. Phosphorus fertilizer has increasingly become a bottleneck restricting the sustainable development of my country's agriculture. my country is the world's largest consumer of chemical fertilizers and the largest consumer of phosphate fertilizers, with an annual consumption of about 11 million tons of phosphate fertilizers. As we all know, my country is short of phosphate rock resources, with reserves of 15.198 billion tons. In another 20 years, the medium and high-grade phosphate ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/04A01N63/00A01P5/00A01P3/00C05G3/00C05F11/08C12R1/01
Inventor 范丙全
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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