Biocontrol fungus for preventing and controlling plant mycosis and its preparation process

A phytopathogenic and fungal technology, applied in botany equipment and methods, plant growth regulators, chemicals for biological control, etc., can solve the problem of high development costs of new varieties of fungicides, toxicity of wild and non-target organisms, New varieties of fungicides are expensive, etc., to achieve the effect of no poisonous harm to humans and animals, no phytotoxicity to crops, and stable product quality

Inactive Publication Date: 2009-07-15
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemically synthesized fungicides have the advantages of high control efficiency and convenient application, they have the following disadvantages: ①The problem of resistance is serious: especially the resistance of plant pathogenic fungi to systemic fungicides develops quickly and has a high level of resistance
② The cost of developing new fungicides is expensive: due to the increasingly stringent requirements for fungicides, the cost of developing new fungicides is also increasing
③Environmental pollution: Although chemically synthesized fungicides are less harmful to humans than chemically synthesized insecticides, chemically synthesized fungicides also have carcinogenic effects, are toxic to wild and non-target organisms, and have poor environmental compatibility.
[0006] Acinetobacter baumannii is a common endophytic bacterium in plants, but little research has been done on its biological function

Method used

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  • Biocontrol fungus for preventing and controlling plant mycosis and its preparation process
  • Biocontrol fungus for preventing and controlling plant mycosis and its preparation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Isolation and identification of LCH0606 strain

[0018] Fresh camphor tree stems are collected from Zijin Mountain in Nanjing, Jiangsu Province, washed with tap water, dried and cut into small pieces of about 1cm. Sequentially immerse in 75% ethanol and 40% formaldehyde solution to disinfect the surface for 3 minutes each, then wash 3 times with sterile water to remove surface contamination bacteria, then cut the stem longitudinally with sterile scissors, put the cut side down on the potato sucrose On the agar plate, 5 stem segments per plate, 5 plates in total. Cultivate at 30°C, observe the appearance of new colonies every day for a total of 7 days, pick a single colony, and purify by streaking. In addition, put 2 to 3 uncut stems or leaves in the same potato sucrose medium plate as a control to test whether the surface sterilization is thorough. The obtained strains were preserved in a -80°C refrigerator by glycerin preservation method for future use.

...

Embodiment 2

[0021] Embodiment two: the preparation method of Bowman's bacteria liquid

[0022] The seed medium was potato sucrose medium, and a single colony of activated LCH0606 was picked and inoculated into 4 mL potato sucrose medium (15 mL test tube) for culture, and cultured on a shaker at 37° C. and 100 rpm for 12 hours. Inoculate 1% seed solution in 400mL potato sucrose medium shake flask (1L), culture on a shaker at 37°C and 100rpm for 48-60h, and the obtained bacterial solution is centrifuged at 5000-6000rpm to remove bacteria, and then it becomes Baumann's bacterial solution.

Embodiment 3

[0023] Embodiment three: the preparation method of baumannycin

[0024] The Bowmansin solution described in Example 2 was adsorbed by macroporous resin X-5, eluted with 80% ethanol, and dried by rotary evaporation under reduced pressure to obtain baumannin. The amount of macroporous resin used was 1% of the Baumannii solution, and the flow rate of sample loading and elution was 4 mL / min. The yield of baowmanicin was 568mg / L.

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Abstract

The invention belongs to the technical field of biological pesticides. The content includes four aspects: (1) the isolation and identification of Acineto-bacter baumannii LCH0606 strain (Acineto-bacter baumannii LCH0606); (2) the preparation of Baumannii liquid and its application in the control of plant diseases; Preparation and disease prevention application of manmectin; (4) Analysis of active components of manmectin. The antibacterial active ingredient of Bowman's bacteria liquid or baumannin is mainly Iturin A, which has the characteristics of wide bactericidal spectrum, long-lasting effect, less resistance to diseases, no phytotoxicity of plants, biosafety and environmental friendliness, etc. Leaf fungi, especially Anthracnose, Phytophthora, Botrytis cinerea, Fusarium and Rhizoctonia, have significant control effects; in addition, Bowman's liquid or Baumannin can promote the germination of rice seeds to a certain extent.

Description

1. Technical field [0001] The invention belongs to the technical field of biological pesticides. 2. Background technology [0002] Human beings are facing many challenges in the 21st century, and one of the main problems is the problem of population and food. my country's population accounts for a quarter of the world's total population, but the arable land is only 6.8% of the world's arable land. This problem is particularly prominent in our country. Therefore, increasing food production is a strategic task related to people's livelihood in our country. However, according to the survey, 20% of the world's main food crops and economic crops are lost due to diseases every year (Oerke et al, 1994. Crop Protection and Crop Production. Elsevier, Amsterdam, 808pp). my country's grain crops lose at least 10-15% due to diseases. If fungicides are not used at all, the losses of cucumbers in normal years are about 55%-75%, radishes are 40%, sweet baskets are 25%, apples are 30%. 24...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P1/00
Inventor 刘常宏陈欣顾玉诚王艳艳幸颖汤城
Owner NANJING UNIV
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