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Application of pipecolic acid as plant disease resistance activator in the control of apple fruit diseases

A technology of pipecolic acid, a disease resistance activator, is applied in the application field of pipecolic acid as a plant disease resistance activator in the prevention and treatment of apple fruit diseases. It can solve the problems of pesticide residues, difficulties, and disease damage prevention and control, and achieve long-lasting effects. Long-lasting, easy to use, and remarkable preventive effect

Active Publication Date: 2021-10-12
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aiming at the problems of serious damage to apple fruit diseases and difficult prevention and control in current agricultural production, pesticide residues caused by chemical agents and drug resistance of pathogenic bacteria, the present invention provides a method for inducing disease resistance of apple fruits to effectively prevent and control apple fruit diseases

Method used

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  • Application of pipecolic acid as plant disease resistance activator in the control of apple fruit diseases
  • Application of pipecolic acid as plant disease resistance activator in the control of apple fruit diseases
  • Application of pipecolic acid as plant disease resistance activator in the control of apple fruit diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1.1 Effect of pipecolic acid on mycelia growth of apple pathogen

[0038] Prepare PDA medium, add pipecolic acid aqueous solution to it, and make the final concentration 0.5mmol L -1 , in the above-mentioned PDA medium, respectively inoculate the apple ring spot pathogen Botryosphaeria dothidea (Botryosphaeriadothidea) that activates well, Botrytis cinerea (Botrytis cinerea), the anthracnose pathogenic bacteria Colletotrichum gloeosporioides (Colletotrichum gloeosporioides), Spot disease pathogens Acremonium sclerotigenum, heart disease pathogens Trichothecium roseum, Alternaria alternate and Fusarium spp. The above-mentioned bacteria are common pathogenic bacteria in this field. They were cultured at 25°C in constant temperature and dark for 3-5 days, and the colony diameter was measured.

[0039] The above-mentioned PDA medium used has the following components and formula: weigh 200g after peeling the potatoes, cut into small pieces and boil in water for 15-20min, ad...

Embodiment 2

[0046] Control effect of pipecolic acid on apple fruit diseases:

[0047] Configure 0.5mmol·L -1 Pipecolic acid aqueous solution, apple ring spot pathogen Botryosphaeriadothidea, gray mold pathogen Botrytis cinerea, anthracnose pathogen Colletotrichum gloeosporioides, leaf spot pathogen sclerotia Acremonium sclerotigenum, mold heart pathogen Trichothecium roseum, Alternaria alternate and Fusarium spp. were activated and cultured on PDA medium for 3 days, and the diameters were obtained at the edge of the colony 5mm bacteria cake for later use.

[0048] Pick Fuji apples of uniform size and health, wash them with running water, sterilize their surfaces with 75% alcohol, dry them at room temperature, and stab them at the equator of the apple. 0.5mmol·L -1 The pipecolic acid aqueous solution is evenly sprayed on the fruit until there are water droplets dripping, and after drying at room temperature, the above-mentioned pathogenic bacteria cake is inoculated on the punctured sit...

Embodiment 3

[0054] Changes in the control effect of pipecolic acid with the application interval:

[0055] Process apple fruit according to the method in embodiment 2, after causing wound, 0.5mmol L -1 Spray the pipecolic acid aqueous solution evenly on the fruit until there are water droplets dripping, dry at room temperature, and inoculate Botryosphaeria dothidea and Botrytis cinerea at the puncture wounds respectively at 12h, 24h, 48h, 72h and 96h after spraying (Botrytis cinerea) fungus cake, to determine the control effect of pipecolic acid with the change of application interval.

[0056] The result is as figure 2 and image 3 Shown: Inoculation of pathogenic bacteria at different intervals has significant differences in the control effect, and the interval time is 48-72h, the control effect is the best, and the control effect is above 75%.

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Abstract

The invention discloses the application of pipecolic acid as a plant disease resistance activator in the prevention and treatment of apple fruit diseases, and belongs to the technical field of plant induced disease resistance. The pipecolic acid is used to enhance the activity of defense enzymes in plants and increase the expression of disease resistance genes. The defense enzymes are PAL, PPO, POD and SOD, and the disease resistance genes are PR1 and PR5. The concentration is 0.2mmol·L ‑1 ~0.5mmol·L ‑1 The pipecolic acid solution can be used to induce disease resistance of apple fruit and prevent and control apple fruit diseases. The pipecolic acid solution of the present invention itself has no in vitro bactericidal or bacteriostatic activity, and can only induce plant disease resistance under living conditions, and the disease resistance induced by apple fruits has the characteristics of long duration and wide disease resistance spectrum, and Environmentally friendly and safe, it is not suitable to cause drug resistance of pathogenic bacteria; the method of use is simple, the concentration is low, and the dosage is small.

Description

technical field [0001] The invention belongs to the technical field of plant induced disease resistance, and in particular relates to the application of pipecolic acid as a plant disease resistance activator in the prevention and treatment of apple fruit diseases. Background technique [0002] Apple is an important economic crop and export fruit in my country. Its cultivation area and output both rank first in the world. However, the export rate of apples is less than 3% of the total output. Apple diseases, especially fruit diseases, are an important factor causing this phenomenon. The diseases caused by fungal infection in every link of apple production have brought huge losses to people. Apple fruit diseases not only cause fruit rot during the growth period, but also cause rot during post-harvest storage, fresh-keeping and transportation, causing serious economic losses. [0003] Apple fruit diseases mainly include: apple ring spot (Botryosphaeria dothidea), gray mold (Bo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/40A01P21/00
CPCA01N43/40
Inventor 王彩霞孙子豪韩春雪李晓鹏练森李保华
Owner QINGDAO AGRI UNIV
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