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Method for preparing total phenolic acid from lonicera hypoqlauca miq flower

A technology of red glandular honeysuckle and total phenolic acids, applied in the field of biological preparation, can solve the problems of no preparation method and process optimization report, no total phenolic acids, etc.

Inactive Publication Date: 2014-04-16
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many research reports on the extraction and separation of chlorogenic acids from honeysuckle and mountain silver flowers, but there are almost no research reports on the total phenolic acids in Lonicera red glandularis, and there are no reports on the preparation method and process optimization. This study uses solid The phase extraction process was used to separate and purify the total phenolic acids of Lonicerae japonicus, and at the same time optimize the preparation process. It provides a solid foundation for the development and utilization of health food, and provides an experimental basis for the further development and utilization of total phenolic acids of Lonicera Lonicerae and the formulation of quality standards

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] In the ultrasonic extraction stage, select 100g of medicinal materials, add 1200g of absolute ethanol, ultrasonic frequency 200w, ultrasonic time 20min, and then add 600g of absolute ethanol for extraction;

[0039] In the solid phase extraction stage, the concentration of the sample solution is 1.0 mg / mL, the sample loading flow rate is 2.0 L / min, the sample preparation solvent ethanol solution is 60% ethanol solution, the eluent is 60% ethanol solution, and the elution flow rate is 1.5 L / min ;

[0040] As a result of the test, the purity of the total phenolic acids was 88.53%, and the yield was 7.35%.

Embodiment 2

[0042] In the ultrasonic extraction stage, select 100g of medicinal materials, add 1000g of absolute ethanol, ultrasonic frequency 250w, ultrasonic time 30min, and then add 500g of absolute ethanol for extraction;

[0043] In the solid phase extraction stage, the concentration of the sample solution is 1.5 mg / mL, the sample loading flow rate is 1.5 L / min, the sample preparation solvent ethanol solution is 70% ethanol solution, the eluent is 70% ethanol solution, and the elution flow rate is 1.0 L / min;

[0044] As a result of the test, the purity of the total phenolic acids was 89.63%, and the yield was 8.26%.

Embodiment 3

[0046]In the ultrasonic extraction stage, select 100g of medicinal materials, add 800g of absolute ethanol, ultrasonic frequency 300w, ultrasonic time 40min, and then add 400g of absolute ethanol for extraction;

[0047] In the solid phase extraction stage, the sample solution concentration is 2.0 mg / mL, the sample loading flow rate is 1.0 L / min, the sample preparation solvent ethanol solution is 80% ethanol solution, the eluent is 80% ethanol solution, and the elution flow rate is 1.0 L / min;

[0048] As a result of the test, the purity of the total phenolic acids was 87.03%, and the yield was 7.63%.

[0049] By analogy, multiple groups of experiments are done for comparison, and it can be found that the best process conditions are:

[0050] In the ultrasonic extraction stage, the amount of absolute ethanol added as an extraction solvent is 10 times the weight of the medicinal material, the ultrasonic power is 250w, the ultrasonic time is 30min, and the amount of absolute etha...

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Abstract

The invention discloses a method for preparing total phenolic acid from a lonicera hypoqlauca miq flower, and provides a manufacturing technique which uses the lonicera hypoqlauca miq flower as raw materials and uses ultrasonic vibrations to extract and conduct solid-phase extraction so as to prepare the total phenolic acid. Through the steps of pretreatment of the raw materials, ultrasonic extraction, sample concentration, solid-phase extraction and postprocessing, a best technological parameter is chosen and adopted so as to obtain excellent products. A good foundation is laid for development and utilization of the total phenolic acid of the lonicera hypoqlauca miq flower in the aspects of medicine and health food in future, a reference function is provided, and thus the method for preparing the total phenolic acid from the lonicera hypoqlauca miq flower has great significance to promoting the industrialization development of the lonicera hypoqlauca miq flower in China.

Description

technical field [0001] The invention relates to biological preparation technology, in particular to a process for separating and purifying total phenolic acids from honeysuckle flowers. Background technique [0002] Lonicera Japonica is a plant of the Lonicera family. It is a commonly used traditional Chinese medicine in my country. It has the functions of clearing heat and detoxifying, antibacterial and anti-inflammatory, protecting the liver and promoting gallbladder. It is often used as medicine with dried buds or early flowers. Listed in the 2005 edition of the Chinese Pharmacopoeia under the item "Mountain Silver Flower", the red glandular honeysuckle is rich in resources and is mainly produced in Henan, Shandong, Yunnan, Guangxi and other places, and is one of the main sources of medicinal materials for Sanyinhua. Mountain silver flower has inhibitory effect on a variety of pathogenic bacteria. It is a broad-spectrum antibacterial traditional Chinese medicine and has ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/355A61P31/04A23L1/29A23L33/00
Inventor 舒孝顺卿婉华许凯扬杨晓平刘瑞兴龚福春陶钧
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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