Method for preparing total phenolic acid from lonicera hypoqlauca miq flower
A technology of red glandular honeysuckle and total phenolic acids, applied in the field of biological preparation, can solve the problems of no preparation method and process optimization report, no total phenolic acids, etc.
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Embodiment 1
[0038] In the ultrasonic extraction stage, select 100g of medicinal materials, add 1200g of absolute ethanol, ultrasonic frequency 200w, ultrasonic time 20min, and then add 600g of absolute ethanol for extraction;
[0039] In the solid phase extraction stage, the concentration of the sample solution is 1.0 mg / mL, the sample loading flow rate is 2.0 L / min, the sample preparation solvent ethanol solution is 60% ethanol solution, the eluent is 60% ethanol solution, and the elution flow rate is 1.5 L / min ;
[0040] As a result of the test, the purity of the total phenolic acids was 88.53%, and the yield was 7.35%.
Embodiment 2
[0042] In the ultrasonic extraction stage, select 100g of medicinal materials, add 1000g of absolute ethanol, ultrasonic frequency 250w, ultrasonic time 30min, and then add 500g of absolute ethanol for extraction;
[0043] In the solid phase extraction stage, the concentration of the sample solution is 1.5 mg / mL, the sample loading flow rate is 1.5 L / min, the sample preparation solvent ethanol solution is 70% ethanol solution, the eluent is 70% ethanol solution, and the elution flow rate is 1.0 L / min;
[0044] As a result of the test, the purity of the total phenolic acids was 89.63%, and the yield was 8.26%.
Embodiment 3
[0046]In the ultrasonic extraction stage, select 100g of medicinal materials, add 800g of absolute ethanol, ultrasonic frequency 300w, ultrasonic time 40min, and then add 400g of absolute ethanol for extraction;
[0047] In the solid phase extraction stage, the sample solution concentration is 2.0 mg / mL, the sample loading flow rate is 1.0 L / min, the sample preparation solvent ethanol solution is 80% ethanol solution, the eluent is 80% ethanol solution, and the elution flow rate is 1.0 L / min;
[0048] As a result of the test, the purity of the total phenolic acids was 87.03%, and the yield was 7.63%.
[0049] By analogy, multiple groups of experiments are done for comparison, and it can be found that the best process conditions are:
[0050] In the ultrasonic extraction stage, the amount of absolute ethanol added as an extraction solvent is 10 times the weight of the medicinal material, the ultrasonic power is 250w, the ultrasonic time is 30min, and the amount of absolute etha...
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