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Method for separating beta-estradiol and oestrone based on nanometer channel modified by aptamer

A nanochannel and nucleic acid aptamer technology, applied in the fields of steroids, organic chemistry, etc., can solve the problems of complex, time-consuming, long cycle, etc., and achieve the effect of high selective separation coefficient, good application prospect and simple separation process.

Inactive Publication Date: 2013-06-05
SHANGHAI NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, these methods are complex, time-consuming, inconvenient to carry, and some require derivatization of the sample, which takes a long time

Method used

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  • Method for separating beta-estradiol and oestrone based on nanometer channel modified by aptamer
  • Method for separating beta-estradiol and oestrone based on nanometer channel modified by aptamer
  • Method for separating beta-estradiol and oestrone based on nanometer channel modified by aptamer

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Embodiment 1

[0029] Separation of a mixture of β-estradiol (E2) and estrone (E1)

[0030] The prepared concentration is 1.7647×10 -5 mol / L mixed solution of β-estradiol (E2) and estrone (E1) (pH=7.4, in Tris-HCl buffer solution), add 8 mL of prepared β-estradiol to the injection pool (E2) and estrone (E1) mixed solution, add the Tris-HCl buffer solution of the same concentration without the substance to be analyzed into the osmosis cell. Under the action of stirring, the separation of the two is carried out by using a nanochannel membrane with a pore size of about 20 nm. The high performance liquid chromatography was used to detect the content of the substances in the permeation pool every 2 hours. The number of samples was 5 times, and the migration time was 12 hours.

[0031] Depend on figure 2 (c) and figure 2 (d) It can be seen that gold nanotubes with good shape can be obtained by adopting the method of the present invention.

[0032] Depend on image 3 It can be seen that the...

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Abstract

The invention discloses a method for separating beta-estradiol and oestrone method based on a nanometer channel modified by an aptamer. A prepared array gold nanoparticle channel is used as a carrier and aptamer modifies beta-estradiol inside the gold nanoparticle channel so as to obtain a gold nanoparticle channel membrane which has special selectivity to the beta-estradiol. A difference of the beta-estradiol and the oestrone is utilized, transfer speed of the beta-estradiol and the oestrone in the nanometer channel which modifies the aptamer, the aptamer has the selectivity to the beta-estradiol, and comparatively passes through the modified nanometer channel, but the oestrone is not easy to pass through, and the separation of the beta-estradiol and the oestrone is achieved. The method can provide a novel idea for conducting compartment analysis to estrogens with different affinity of other aptamers.

Description

technical field [0001] The invention relates to a molecular level membrane separation technology based on a nanochannel membrane material, in particular to a method for separating β-estradiol and estrone based on a nanochannel modified by a nucleic acid aptamer; the field of materials and the field of separation technology. Background technique [0002] There is a phenomenon of abuse of hormones in the process of raising food animals and producing cosmetics, which leads to the pollution of antibiotics and hormone residues in meat, eggs, dairy products and cosmetics. A large number of literatures show that the intake of these hormones, especially estrogens, may cause endocrine-related tumors and birth defects, damage the liver and kidneys, and seriously affect the growth and development of infants and adolescents; in addition, hormones have Special beauty effects are often illegally added to various cosmetics by some cosmetics manufacturers, which poses a potential threat to ...

Claims

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Application Information

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IPC IPC(8): C07J1/00
Inventor 黄杉生代国帅李平樊定艳
Owner SHANGHAI NORMAL UNIVERSITY
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