Molecular biological method of identifying trachinotus ovatus

A technology of molecular biology and oval pomfret is applied in the field of molecular biology for identifying oval pomfret, and can solve the problems of difficult identification, high cost, large error and the like

Inactive Publication Date: 2013-06-05
SHANTOU UNIV MEDICAL COLLEGE
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Problems solved by technology

This makes identification difficult and error-prone in situations such as juvenile fish, broken fish, or decay
In recent years, many laboratories at home and abroad have begun to use mitochondrial DNA typing, restriction length polymorphism, isoenzyme electroph

Method used

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Embodiment Construction

[0026] A pair of specific primer pairs for screening the transcriptional spacer DNA fragments between ribosomal 5.8SrRNA and 28SrRNA genes of Perciformes fishes, as a general primer pair for Perciformes fishes, the primer pair is: Forward primer: 5'- gtcgatgaagaacgcagcta-3'; reverse primer: 5'-gtcttctttccccgctgatt-3'-.

[0027] Common and rare fishes in the order Perciformes: short-tailed walleye, red sea bream, flat bream, black bream, yellowfin bream, silver pomfret, oval pomfret, Japanese golden thread fish, golden thread fish, perch, mandarin fish , large yellow croaker, red-spotted grouper, barracuda, barramundi, light sea bream, long-tailed walleye, cobia, blue round trevally, black pomfret, short-bodied silver perch and other fish fresh muscle 3mg, digestion Overnight, extracted by phenol-chlorine method, alcohol precipitation, air-dried, dissolved in double distilled water and stored at -20°C for later use.

[0028] The previously screened universal primer pairs were ...

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Abstract

The invention relates to a molecular biological method of identifying trachiontus ovatus. The method of identifying the trachinotus ovatus comprises steps as below: designing a general primer pair of perciformes fish; carrying out augmented sequencing of deoxyribonucleic acid (DNA) samples of common valuable and rare perciformes fish through the general primer pair; selecting unique DNA fragments of each kind of common valuable and rare perciformes fish according to the sequencing result; screening out one specific primer of the trachinotus ovatus out of the DNA fragments; matching one primer of the general primer pair with a specific primer of the trachinotus ovatus to form a specific primer pair of the trachinotus ovatus; carrying out augmentation of the samples of the common valuable and rare perciformes fish through the specific primer pair of the trachinotus ovatus; mixing the general primer pair with one specific primer pair of the trachinotus ovatus to form a mixed primer; carrying out augmentation of the DNA samples of fish needing to be identified by using the mixed primer pair; obtaining the species identification result of samples according to comparison between the electrophoretic band and the identification standard electrophoretic band.

Description

technical field [0001] The invention relates to a method for identifying common and rare perciformes, in particular to a molecular biology method for identifying pomfret ovata. Background technique [0002] To protect biodiversity is to protect human beings. Due to the particularity of the water ecological environment and the fact that most aquatic animals have been caught by people, fish biodiversity is more vulnerable than terrestrial animal diversity and is under greater pressure. To protect fish germplasm resources is to protect the production source of aquatic animal protein necessary for human survival. Fish resource is a renewable resource. As long as it is used properly, it can be inexhaustible and enduring for a long time. However, if it is overfished or overfished, the resource may be exhausted or the individual will become smaller and the quality will decrease. Fishing is not a negative factor. As long as fishing is reasonable, it can maximize the production of ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 袁万安于晓军游翠红
Owner SHANTOU UNIV MEDICAL COLLEGE
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