Molecular biological method of identifying trachinotus ovatus

Abstract

The invention relates to a molecular biological method of identifying trachiontus ovatus. The method of identifying the trachinotus ovatus comprises steps as below: designing a general primer pair of perciformes fish; carrying out augmented sequencing of deoxyribonucleic acid (DNA) samples of common valuable and rare perciformes fish through the general primer pair; selecting unique DNA fragments of each kind of common valuable and rare perciformes fish according to the sequencing result; screening out one specific primer of the trachinotus ovatus out of the DNA fragments; matching one primer of the general primer pair with a specific primer of the trachinotus ovatus to form a specific primer pair of the trachinotus ovatus; carrying out augmentation of the samples of the common valuable and rare perciformes fish through the specific primer pair of the trachinotus ovatus; mixing the general primer pair with one specific primer pair of the trachinotus ovatus to form a mixed primer; carrying out augmentation of the DNA samples of fish needing to be identified by using the mixed primer pair; obtaining the species identification result of samples according to comparison between the electrophoretic band and the identification standard electrophoretic band.

Description

technical field [0001] The invention relates to a method for identifying common and rare perciformes, in particular to a molecular biology method for identifying pomfret ovata. Background technique [0002] To protect biodiversity is to protect human beings. Due to the particularity of the water ecological environment and the fact that most aquatic animals have been caught by people, fish biodiversity is more vulnerable than terrestrial animal diversity and is under greater pressure. To protect fish germplasm resources is to protect the production source of aquatic animal protein necessary for human survival. Fish resource is a renewable resource. As long as it is used properly, it can be inexhaustible and enduring for a long time. However, if it is overfished or overfished, the resource may be exhausted or the individual will become smaller and the quality will decrease. Fishing is not a negative factor. As long as fishing is reasonable, it can maximize the production of ...

AI Technical Summary

Problems solved by technology

This makes identification difficult and error-prone in situations such as juvenile fish, broken fish, or decay

In recent years, many laboratories at home and abroad have begun to use mitochondrial DNA typing, restriction length polymorphism, isoenzyme electrophoresis, and random primer amplification polymorphism (RAPD) techniques to identify fish, but these methods are generally cumbersome and time-consuming. , laborious, expensive, not conducive to operation, poor repeatability, large errors and other disadvantages