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Method for extracting D-ribose from fermentation liquor by ultrafiltration and ion exchange technologies

An ion exchange and fermentation broth technology, which is applied in the field of extracting D-ribose from fermentation broth by using ultrafiltration and ion exchange technologies, can solve the problems of short process route, easy blockage of step membrane flux, etc., and achieves high extraction separation, Simplify the multi-stage membrane separation process and reduce the effect of extraction and separation steps

Inactive Publication Date: 2013-06-12
JIANGXI CHENGZHI BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process operation of the present invention is simple and easy, and the process route is short, which overcomes the defects of many steps, rapid decrease in membrane flux and easy clogging existing in the application process of the existing membrane separation technology, and comprehensively improves the quality and yield of D-ribose products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 100L of D-ribose fermentation broth passes through a microfiltration membrane with a membrane pore size of 1000nm, the membrane entry pressure is 0.2MPa, the membrane exit pressure is 0.1MPa, and the operating temperature is 25°C. After the fermentation broth is concentrated by 10 times, 20L of dialysis water is added. The filtrate passes through the 732-type cation exchange resin column at a flow rate of 2BV / H, and the effluent from the positive column is also directly connected to the 717-type anion-exchange resin column at a flow rate of 2BV / H. The ion exchange liquid passes through the ultrafiltration membrane with a molecular weight cut-off of 5000, the operating pressure is 1.4MPa, and the operating temperature is 30-35°C. After the ion exchange liquid is concentrated 15 times, 25L of dialysis water is added. The filtrate passes through 732-type cation exchange resin, 331-type anion-exchange resin, and HZ-803 resin in sequence. The resin chromatography effluent is...

Embodiment 2

[0023] 100L of D-ribose fermentation broth passed through a microfiltration membrane with a membrane pore size of 200nm, the membrane entry pressure was 0.2MPa, the membrane exit pressure was 0.1MPa, and the operating temperature was 25°C. After the fermentation broth was concentrated 10 times, 30L of dialysis water was added. The filtrate passes through the 732-type cation exchange resin column at a flow rate of 2BV / H, and the effluent from the positive column is also directly connected to the 717-type anion-exchange resin column at a flow rate of 2BV / H. The ionotropic solution passes through the ultrafiltration membrane with a molecular weight cut-off of 1000, the operating pressure is 1.4MPa, and the operating temperature is 30-35°C. After the ionotropic fluid is concentrated 15 times, 30L of dialysis water is added. According to the subsequent extraction method in Example 1, after the D-ribose crystals were vacuum-dried, a total of 4.93 Kg of D-ribose product was obtained. ...

Embodiment 3

[0025] 100L of D-ribose fermentation broth passes through a microfiltration membrane with a membrane pore size of 500nm, the membrane entry pressure is 0.2MPa, the membrane exit pressure is 0.1MPa, and the operating temperature is 25°C. After the fermentation broth is concentrated 8 times, 25L of dialysis water is added. The filtrate passes through the 732-type cation exchange resin column at a flow rate of 2BV / H, and the effluent from the positive column is also directly connected to the 717-type anion-exchange resin column at a flow rate of 2BV / H. The ionotropic solution passes through the ultrafiltration membrane with a molecular weight cut-off of 2000, the operating pressure is 1.4MPa, and the operating temperature is 30-35°C. After the ionotropic fluid is concentrated 15 times, 25L of dialysis water is added. The ultrafiltrate passes through 732-type cation exchange resin, 331-type anion-exchange resin, and HZ-803 resin in sequence. The resin chromatography effluent is co...

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Abstract

The invention discloses a method for extracting D-ribose from fermentation liquor by ultrafiltration and ion exchange technologies. The method comprises the following steps of: (1) micro-filtration of fermentation liquor, namely, removing a large part of thallus, large protein and impurity particles in the D-ribose fermentation liquor through a filter membrane of which the membrane pore size is 50-1000nm; (2) ion exchange of filter liquid, namely, removing multivalent ions in the filter liquid through cation and anion exchange columns; (3) ultrafiltration of ion exchange liquid, namely, passing the ion exchange liquid through the filter membrane of which the molecular weight cut off is 500-5000; and (4) charomatography, concentration and crystallization of the filter liquid. The method has the advantages that the existing multi-stage membrane separation procedure is simplified by a secondary membrane separation process; the extraction separation step is reduced; the ion exchange technology is applied into twice membrane separation; the defects of drop of membrane flux caused by improvement of concentration multiple in the membrane separation process in actual production and separation of a metal salt material are well solved; and the method has the advantages of simple and reasonable process, high extraction and separation degree, high yield and the like, and is simple and convenient to operate.

Description

technical field [0001] The invention relates to a method for directly extracting D-ribose from fermentation broth, in particular to a method for extracting D-ribose from fermentation broth by ultrafiltration and ion exchange technology. Background technique [0002] D-ribose exists in all biological cells as a component of the genetic material ribonucleic acid (RNA) and deoxyribonucleic acid (DNA). Its reducing body, ribitol, is an important component of ribitol teicolic acid in the cell membrane, and plays an important role in physiology. [0003] D-ribose is industrially synthesized vitamin B 2 It is an important raw material and an important intermediate of many nucleic acid drugs. Especially in recent years, the demand for D-ribose as a synthetic raw material for anticancer and antiviral drugs, riboflavin and flavor enhancers has gradually increased in the pharmaceutical and food industries. With the in-depth research on D-ribose, the role of D-ribose in health care h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/02C07H1/06
Inventor 杨光郑雄敏杨世传曾乐黄恒述任祥万
Owner JIANGXI CHENGZHI BIOENG
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