Halomonas sulfidaeris and application thereof

A technology of Pseudomonas halophilus and bacteria powder, applied in Pseudomonas halophilus and its application fields, can solve the problems of destroying the structure and function of the ecosystem, increasing the degree of water quality deterioration, etc., and achieve simple nutritional needs and application prospects broad effect

Active Publication Date: 2014-05-14
青岛蔚蓝龙初生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The decomposition of algae will also produce a large amount of toxic gases and toxins, which will seriously increase the deterioration of water quality and destroy the structure and function of the entire ecosystem.

Method used

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  • Halomonas sulfidaeris and application thereof
  • Halomonas sulfidaeris and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Isolation, screening and identification of Pseudomonas halophilus YZ7

[0012] 1. Experimental materials

[0013] Microcystis aeruginosa (Microcystis aeruginosa) FACHB469 among cyanobacteria was selected. After the algae species were activated, they were cultured at 25° C., with a light intensity of 2500 lx and a light-dark ratio of 12h:12h.

[0014] The enrichment culture of algae-lytic bacteria adopts beef extract peptone medium: beef extract 3g, peptone 10g, NaCl 5g, distilled water 100mL.

[0015] LB medium: peptone 10g, yeast extract 5g, NaCl 5g, distilled water 100mL.

[0016] BG11 modified medium for algae culture: NaNO 3 150mg, K 2 HPO 4 4 mg, MgSO 4 7H2O7.5mg, CaCl 2 2H2O3.6 mg, Na 2 Si0 3 9H 2 O5.8mg, citric acid 0.6mg, ferric ammonium citrate 0.6mg, EDTA0.1mg, Na 2 CO 3 2mg, A5 solution 0.1mL, distilled water 99.9mL.A5 solution: H 3 BO 3 286mg, MnCl 2 4H 2 O181mg, nSO 4 ·7H 2 O22mg, CuSO 4 ·5H 2 O7.9 mg, Na 2 MoO 4 · 2H203.9mg,...

Embodiment 2

[0028] Embodiment 2: Inhibition experiment of Pseudomonas halophilus YZ7 on algae in culture pond

[0029] Pseudomonas halophilus YZ7 was activated, expanded and cultured. After liquid fermentation, the bacteria sludge was collected by centrifugation, added to starch carrier, and dried to make 10 billion / g bacteria powder. The aquaculture pond water was taken from the Penaeus vannamei culture pond in Yancheng, Jiangsu Province, and the total algae density was 3.42×10 after microscopic examination and counting. 4 cells / mL, wherein Microcystis aeruginosa is the dominant algae species, the dominance is 43.19%, the experimental container is a 1mL beaker, and the experimental water body is 500mL. The experimental group adds the above-mentioned bacterial powder according to the mass ratio of 50ppm, and the control group does not add the bacterial powder. Three parallel experiments were set up in the group and the control group, and the experiment was carried out in a light incubator...

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Abstract

The invention aims at providing a halomonas sulfidaeris. The strain is named Halomonas sulfidaeris YZ7, and the strain is preserved in China Center for Type Culture Collection of Wuhan University, Wuhan, China in 6th, March, 2013, and the preservation number of the strain is CCTCC NO:M2013070. Halomonas sulfidaeris YZ7 disclosed by the invention has the capability of inhibiting algae blooms, and the strain is simple in nutrient requirement, so that the strain can be developed for an aquaculture microbial preparation and a waste water treatment agent, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a strain of pseudomonas halophilus and its application. Background technique [0002] In recent years, the outbreak of harmful algal blooms in global water bodies has become more frequent and more serious, and the occurrence locations are all over the world. Therefore, preventing and controlling the occurrence of harmful algae and reducing its harm to the water environment, human living environment, and aquaculture industry have become a major concern of people and countries. [0003] The normal water system is in a dynamic balance of mutual restraint. When the algae bloom occurs, a few types of algae proliferate in large numbers, and thick blue-green aggregates are formed on the water surface. Because harmful algae cover the water surface, the water surface is covered, which reduces the transparency of the water body, changes the color of the water...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 武玉强孙超
Owner 青岛蔚蓝龙初生物科技有限公司
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