Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing ethanol

A technology of ethanol and high-temperature-resistant amylase, which is applied in the field of fermentation to ethanol, can solve the problems of low fermentation efficiency, achieve the effects of improving fermentation efficiency, reducing the consumption of sugar and grain, and inhibiting the growth of microorganisms

Active Publication Date: 2013-06-12
COFCO BIOTECHNOLOGY CO LTD
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thereby making the fermentation efficiency lower when making ethanol by subsequent fermentation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing ethanol

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] The method for preparing ethanol provided by the present invention includes the following steps:

[0021] (1) Prepare an aqueous solution containing high-temperature resistant amylase, and mix the aqueous solution containing high-temperature resistant amylase with starch raw material powder to obtain a mixture. The mixing conditions include a temperature of 65-90°C, preferably 70-85°C;

[0022] (2) Spray the mixture obtained in step (1) once to 90-100°C and hold it; then spray the product obtained by spraying once to 120-140°C and hold it;

[0023] (3) Under enzymatic hydrolysis conditions, the product obtained in step (2) is mixed with amylase for enzymatic hydrolysis to obtain a sugar liquid containing monosaccharides;

[0024] (4) Ferment the sugar liquid obtained in step (3).

[0025] In step (1), the purpose of the present invention can be achieved as long as the mixing conditions are the above-mentioned conditions. Wherein, the method for preparing an aqueous solution cont...

Embodiment 1

[0054] (1) Dissolve heat-resistant amylase with an amount of 25 enzyme activity units / g corn flour in water, and at 70°C, combine the obtained high-temperature amylase solution with 100 parts by weight of corn flour (average particle size of 400μm). ) Mix to obtain a mixture;

[0055] (2) The mixture and saturated steam at 150℃ are injected once in the Zhaoguang ejector (the weight ratio of steam to the mixture is 0.05:1), and the injection time is 5s, so that the temperature of the product after contact with the steam is 90℃, and the laminar flow is maintained for 120 minutes; the product obtained by the first injection and the supersaturated steam at 220℃ are injected again in the mega-injector (the weight ratio of steam to the product obtained by the first injection is 0.05:1), The time of the second injection is 5s, so that the temperature of the product after contact with steam is 120°C, and it is kept at this temperature for 4 minutes;

[0056] (3) Flash vaporize the product...

Embodiment 2

[0060] (1) Dissolve heat-resistant amylase with an amount of 35 enzyme activity units / g corn flour in water, and at 85°C, combine the obtained high-temperature amylase aqueous solution with 100 parts by weight of corn flour (average particle size of 400μm). ) Mix to obtain a mixture;

[0061] (2) The mixture and the saturated steam at 160℃ are injected once in the Zhaoguang ejector (the weight ratio of the steam to the mixture is 0.05:1), and the injection time is 5s, so that the temperature of the product after contact with the steam is 100℃, and the laminar flow is maintained for 60 minutes; the product obtained by the first injection and the supersaturated steam at 220℃ are injected again in the mega-injector (the weight ratio of steam to the product obtained by the first injection is 0.06:1), The second injection time is 5s, so that the temperature of the product after contact with the steam is 140°C, and it is kept at this temperature for 10 minutes;

[0062] (3) Flash vapori...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing ethanol, which comprises the following steps of: (1) preparing aqueous solution containing high-temperature resistant amylase, and mixing the aqueous solution containing the high-temperature resistant amylase with starchy material powder to obtain a mixture, wherein mixing is carried out at 65-90 DEG C, preferably 70-85 DEG C; (2) carrying out one-time injection of the mixture obtained in the step (1) to 90-100 DEG C and keeping, and carrying out two-time injection of the product obtained through one-time injection to 120-140 DEG C and keeping; (3) mixing the product obtained in the step (2) with amylase under the condition of enzymatic hydrolysis, and carrying out enzymatic hydrolysis to obtain liquid glucose containing monosaccharide; and (4) fermenting the liquid glucose obtained in the step (3). By means of the technical scheme, the method disclosed by the invention is capable of effectively inhibiting growth of micro-organisms in the size mixing process and effectively increasing the fermentation efficiency of ethanol; and the liquefaction effect is steady and thorough; the starch utilization rate is high; and the grain consumption for producing sugar is low.

Description

Technical field [0001] The invention relates to a method for preparing ethanol, in particular to a method for preparing ethanol by fermentation using the sugar liquid prepared in the process of the invention. Background technique [0002] At present, the method of producing ethanol by fermentation in domestic enterprises is generally as follows: preparing sugar liquid, and using the obtained sugar liquid to prepare a fermentation medium, inoculating yeast into the fermentation medium for fermentation, and obtaining an ethanol fermentation liquid. The method of preparing the sugar solution includes adding corn flour to water at a temperature of 50-60°C for mixing to obtain a slurry, then adding calcium hydroxide to adjust the pH of the slurry to 6.0-6.5, adding amylase, and spraying it to 80 at a time. -95℃, after maintaining the iodine test to pass, press filtration to prepare the liquefied clear liquid, and then contact the obtained liquefied clear liquid with the glucoamylase f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P7/06C12P19/14C12R1/865
CPCY02E50/10
Inventor 张军华罗虎满云朱继成张夕
Owner COFCO BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com