Culturing medium for brown mushroom liquid spawn culturing and formula thereof

A technology of liquid strains and culture substrates, applied in the fields of application, botany equipment and methods, fertilizer mixtures, etc., can solve the problems that liquid strains are not suitable for direct inoculation, storage and transportation are inconvenient, and achieve wide source of culture raw materials, The effect of wide temperature range for mushroom fruiting and simple cultivation technology

Inactive Publication Date: 2013-06-19
天津市金三农农业科技开发有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, liquid strains are not suitable for direct inoculation on large quantities of compost culture materials, because storage and transportation are inconvenient

Method used

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  • Culturing medium for brown mushroom liquid spawn culturing and formula thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Test materials and methods

[0029] Test strain:

[0030] The brown mushroom MC441 was quoted from the Fujian mushroom bacteria research and promotion station, which was sold outside.

[0031] Testing training substrate:

[0032] (1) Landscape cultivation substrate: Grass charcoal soil culture substrate (G / L): 600g of grassy soil, 20g of glucose, 100g of wheat grains, 1.5g of magnesium sulfate, 1g of potassium dihydrogen phosphate, 2g of protein, 1000ml of water.

[0033] (2) Seed culture substrate (G / L): potato 200, glucose 20, protein 胨 2, potassium dihydrogen phosphate 2, magnesium sulfate 1, VB, VB 1 0.01, water 1000ml, pH naturally.

[0034] (3) Basic fermentation training substrate:

[0035] A.PDA medium: 200 g potatoes, 20 g glucose, 1 000 ml of water.

[0036] B.Wheat -grained medium: 100 g of potatoes, 1.5 g of seven water sulfate, 1 g potassium dihydrogen phosphate, 2 g of protein, 1000 ml of water.

[0037] C.Wheat -grained medium: 200 g of wheat grains, 1.5 g...

Embodiment 2

[0089] (1) Preparation of seed liquid, take 5 pieces of 0.5 cm 2 Fresh sloping bacteria are inoculated in seed medium (50ml / 250ml triangle bottle), 25 ° C, 150rpm shake in bed for 3 days; the slope training substrate: Grass charcoal soil cultivation substrate (G / L): 600g of grass charcoal soil, glucose 20g glucose 20g 20g, 100g of wheat grains, 1.5g of magnesium sulfate, 1g of potassium dihydrogen phosphate, 2g of protein, 1000ml of water; seed culture substrate (G / L): potato 200, glucose 20, protein pupa 2, potassium dihydrogen phosphate 2, magnesium sulfate 1., 1.VB 1 0.01, water 1000ml, pH naturally;

[0090] (2) Preparation of liquid bacteria, move the seed liquid into 50ml bran fermentation medium with 10%(V / V) inoculation amount (G / L): bran 120, glucose 15, protein 胨 2; 250ml triangle bottleAmong them, 25 ° C, 150rpm cultivated for 7 days, that is, brown mushroom liquid bacteria;

[0091] (3) Fermentation conditions: then transfer to the fermented tank with a 10%inoculation ...

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Abstract

The invention provides a culturing medium for brown mushroom liquid spawn culturing and a culturing method of brown mushroom liquid spawn. The culturing medium is characterized by comprising bran fermentation culturing medial (g / L) components, wherein the components comprise 120 grams of bran, 15 grams of glucose and 2 grams of peptone. By the adoption of the culturing medium, quantity of the brown mushroom liquid spawn is improved to 32.043g / L from 1.543g / L, and spawn dry weight in a fermentation tank verification test reaches 38.486g / L. The culturing method satisfies requirements to novel mushroom types and edible mushroom new technology from people and the market. If the culturing method can be widely applied, large economical benefits and social benefits can be produced for sure.

Description

Technical field [0001] The present invention provides a training method involving brown mushrooms, and more specifically, a bran fermentation culture and culture method for the cultivation of brown mushroom liquid bacteria. Background technique [0002] Brown mushroom ( Agaricus Brunnescens PECK), a rare variety in mushrooms, belongs to BasidiomyCotina, Agaritales, Agaricaceae, and mushrooms ( garicus To.Brown mushrooms are commonly known as brown mushrooms, which are Boheme sub -species.The strain has high yield, excellent mushroom quality, and tender taste. Its mushroom cover is naturally brown, the stalk is white, and the meat is densely tight.Now all white bisee mushrooms are white varieties of brown mushrooms, which means that brown mushrooms are wild ancestors of white bisopular mushrooms.Brown mushrooms, alias white mushrooms and mushrooms, belong to the bacteria, umbrella mushrooms, mushroom family, mushrooms.Brown mushrooms are rich in nutrition and delicious. It is repo...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00C05G3/00
Inventor 班立桐黄亮王玉杨丽维孙茜孙建成
Owner 天津市金三农农业科技开发有限公司
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