An automatic cell culture device capable of simulating internal environments of organisms

Abstract

The invention belongs to the technical field of cell culture, particularly discloses an automatic cell culture device capable of simulating internal environments of organisms. The automatic cell culture device comprises a laminar flow sterile sealed chamber. An internal environment simulation tissue and organ culture device and a target cell induction culture device are arranged in the sealed chamber. The automatic cell culture device can simulate the internal environments including a low-oxygen low-permeability environment for culturing embryonic stem cells, a testicular internal environment during maturation of germ cells, a thymus environment for inducing maturation of T cells, a cerebrospinal fluid environment for inducing differentiation of neural stem cells and tissue and organ environments such as livers, pancreas, bone marrow and tumors for inducing differentiation and maturation of corresponding stem cells, and cultures specific high-quality cells in an induction mode to meet needs of cell basic researches and applications. Besides, the simulated internal environments provide a basis for researching an organism cell growth, differentiation and maturation mechanisms. Therefore, the improvement of cell culture quality is facilitated, repeatability of cytology researches is remarkably increased, cost of cell culture is saved, clinic transformation and application of the cell culture technology are quickened, and a good experiment platform is provided for scientific research basic workers.

Description

technical field [0001] The invention belongs to the technical field of cell culture. Specifically disclosed is an automatic cell culture device for simulating the internal environment of a body. Background technique [0002] Cell culture is the basis of bioengineering, biopharmaceuticals, sustainable utilization of biological resources and cell medicine. A sound cell culture technology system is the key to scientific research, production and medical applications. Cells in the body live in different environments during the process of growth and differentiation, and their biological characteristics will also change with the environment. To cultivate cells that truly reflect the characteristics of the body in vitro, a suitable biological environment is required. For example, the hypoxic and hypotonic environment of embryonic stem cells, the testicular environment during germ cell development and maturation, the thymus environment for inducing T cell maturation, the bone marro...

AI Technical Summary

Problems solved by technology

The growth and differentiation of cells depends on the specific growth environment of the body and the interaction between cells, such as the hypoxic and hypotonic environment of embryonic stem cells, the internal testis environment during the development and maturation of germ cells, the thymus environment that induces T cell maturation, multipotent Stem cells differentiate into neural stem cells and nerve cells in the brain and spinal cord environment, etc. However, the current cell culture device is not well designed to simulate the internal environment of the body, and it is difficult to meet the specific cell culture requirements

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