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Method for cultivating Bacillus sp. gxw4-2, and method for controlling pests through using Bacillus sp. gxw4-2

A cultivation method, Bacillus technology, applied in the field of microorganisms, can solve the problems of strong drug resistance, great harm, poor control effect, etc., and achieve the effect of strong regeneration ability and abundant supply

Active Publication Date: 2015-02-18
镇江市润宇生物科技开发有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the major lepidopteran pests such as diamondback moth, Spodoptera litura, rice leaf roller, and tree moth, which are very harmful, highly resistant to pesticides, and poorly controlled by general pesticides in current crop production, the cultivation and Its method of controlling pests creates a new biopesticide

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  • Method for cultivating Bacillus sp. gxw4-2, and method for controlling pests through using Bacillus sp. gxw4-2
  • Method for cultivating Bacillus sp. gxw4-2, and method for controlling pests through using Bacillus sp. gxw4-2
  • Method for cultivating Bacillus sp. gxw4-2, and method for controlling pests through using Bacillus sp. gxw4-2

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Embodiment Construction

[0013] 1. Bacillus sp. GXW4-2 cultivation steps

[0014] (1) Dissect the corpses of diamondback moth, Spodoptera litura, rice leaf roller, and tree moth that died of disease, and isolate and extract Bacillus strain GXW4-2.

[0015] (2) Put LB medium into a container, add 2% calcium powder by weight, and sterilize. When the temperature of the culture container is 28-32° C., inoculate the bacillus GXW4-2 in the culture container, and cultivate it in a biochemical incubator at 28-32° C. for 24 hours to obtain colonies of the bacillus GXW4-2.

[0016] (3) After boiling the LB medium without adding agar, filter it, add 2% calcium powder in the filtrate, and obtain the culture medium after sterilization. When the culture medium is cooled to 32-36°C, inoculate the spores obtained in step (2) into the culture medium under aseptic conditions, in an environment of 13-15% dissolved oxygen, PH6-8, and a temperature range of 32-36°C Ferment for 36-38 hours to obtain ≥10.5 billion spores / ...

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Abstract

The invention relates to a method for cultivating Bacillus sp. gxw4-2 having pest controlling and killing effects, and a method for controlling pests through using the Bacillus sp. gxw4-2, and belongs to the technical field of microbes. The Bacillus sp. gxw4-2 is cultivated through a secondary culture method and is used as a pesticide. Results of tests show that the Bacillus sp. gxw4-2 can effectively inhibit and control the harms of lepidoptera pests to crops, is an ideal microbial pesticide, has the characteristics of fast pest killing speed, good control effect, no pollution to the environment, and non-toxicity to organisms expect target pests, and has the advantages of fast strain cultivation speed, low preparation production cost, no discharge of three wastes, environmental protection, and safety.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to extracting bacillus GXW4-2 (named in Latin as Bacillus sp. The insecticide is diluted and sprayed on the epidermis of plants for the control of plant Lepidoptera pests. Background technique [0002] Plutella xylostella, Spodoptera litura, rice leaf roller, tree moth and other major Lepidoptera pests are common crop pests with strong resistance to pesticides. Those with mild damage will cause a decline in crop biological and economic yields, and those with severe damage will cause crop particles. No income. In recent years, many developed countries in the world have invested considerable manpower and financial resources in the development of biopesticides, but the development of biofungicide products is more, and the development of new biopesticide products is not progressing fast. As far as the application of biopesticides to major lepidopteran pests such as diamon...

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P7/04C12R1/07
Inventor 高小文
Owner 镇江市润宇生物科技开发有限公司