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Detection agent composition for epithelial tissue tumor cells and preparation method thereof

A technology of composition and detection agent, applied in the field of detection agent composition, to achieve the effects of high sensitivity, easy acquisition and high specificity

Active Publication Date: 2013-07-03
闫文广
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there is no report on the combination of methylene blue redox color change reaction with folic acid and folic acid complex to quickly locate and detect epithelial tumor cells through staining and color change reactions

Method used

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  • Detection agent composition for epithelial tissue tumor cells and preparation method thereof
  • Detection agent composition for epithelial tissue tumor cells and preparation method thereof
  • Detection agent composition for epithelial tissue tumor cells and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Dissolve medicinal-grade folic acid in an aqueous solvent with the components specified in Table 1 at room temperature and pressure, add the biological detection agent methylene blue and stir to dissolve, add the reducing agent glucose and stir for 30 minutes, then add analytical pure acetic acid and stir. Mix to make a detection agent composition. Dip the detection agent composition with a large cotton swab, smear it on the cervical epithelial tissue, immediately remove the cotton swab, and immediately observe the color change of the cotton swab. The color of the cotton swab is light yellow-brown, indicating that there is no lesion in the epithelial tissue; the color of the cotton swab is green or blue Green indicates inflammatory lesions, condyloma acuminatum (HPV virus infection) and CIN I lesions; the color of the cotton swab is purple-black, indicating lesions above CIN II. At the same time, take biopsy from multiple spots in the epithelial tissue and send it for hi...

Embodiment 2

[0054] The method of Example 1 was repeated according to the content of each component specified in Table 1 below, but the folic acid complex was used instead of folic acid. The test results are listed in Table 1.

Embodiment 3

[0056] The method of Example 1 was repeated according to the content of each component specified in Table 1 below, but the folic acid and folic acid complex were co-dissolved in an aqueous solvent. The test results are listed in Table 1.

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Abstract

The invention relates to a detection agent composition for living cells, and specifically relates to a detection agent composition for epithelial tissue tumor cells. The composition contains 0-5% of folic acid, 0-10% of a folic acid compound, 0.01-5% of methylene blue, 0.1-10% of a sugar reducing agent, 2-6% of acetic acid and 3-95% of water. The invention also relates to a preparation method of the detection agent composition and a kit containing the detection agent composition.

Description

Technical field [0001] The present invention relates to a detection agent composition, in particular to a color change-based detection agent composition for epithelial tissue tumor cells. The present invention also relates to a method for preparing the detection agent composition, and a kit containing the detection agent composition. Background technique [0002] At present, the methods used to stain human epithelial tumor cells in vivo mainly include acetic acid whitening reaction, iodine experiment, toluidine blue experiment, methylene blue staining, hematoxylin staining, etc. [0003] Specifically, the acetic acid whitening reaction is to apply an acetic acid solution, such as 3-5% acetic acid solution, on the cervical / vaginal epithelial tissue, and wait for a period of time to observe whether the applied epithelial tissue has an acetic acid whitening reaction area, if there is In the albino area, the epithelial tissue is suspected of having tumor cells. However, although this...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78A61K49/006G01N33/5005G01N33/52G01N2800/7028G01N1/30G01N2001/302
Inventor 闫文广
Owner 闫文广
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