Method for preparing bioactive filler through immobilization of anaerobic ammonia oxidizing bacterium

An anammox bacteria and anammox technology, applied in the field of water treatment, can solve the problems of long doubling time, low cell yield, long residence time, etc., and achieve short time consumption, good sedimentation performance, and recovery of biological activity. quick effect

Active Publication Date: 2013-07-10
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Due to the long doubling time of ANAMMOX bacteria, the cell yield is low (the recognized doubling time is 11d, and the fastest reported doubling time is 4

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Selection and treatment of activated carbon

[0037] Select granular activated carbon with a particle size greater than 1.0mm for mechanical crushing, and retain particles with a particle size of 0.3-0.5mm through sieving, soak in anammox bacteria liquid culture medium for 3 days, and put it in a constant temperature oven at 60°C for drying Dry, ensure that the surface of the granular activated carbon is not wet, take out 240ml of the dried activated carbon and cool it to normal temperature for later use.

[0038] (2) Treatment of anammox bacteria solution

[0039] Concentrate the existing anammox bacteria solution through a centrifuge (12000r / min, 4°C) and divide it into two 20ml bacterial suspensions;

[0040] (3) Acquisition of bacterial embedding solution

[0041] Weigh 2.7g of polyvinyl alcohol, add 10ml of ultrapure water, heat to dissolve for 15min, take it out and stir it evenly, then heat it for 5min, take it out, stir it evenly and cool to room temperatu...

Embodiment 2

[0050] (1) Selection and treatment of activated carbon

[0051] The selection and treatment of activated carbon are the same as in Example (1), and 1000 ml of the dried activated carbon is taken out and cooled to room temperature for later use.

[0052] (2) Treatment of anammox bacteria solution

[0053] The treatment of the anammox bacteria solution was the same as in Example (1), and two 100ml bacterial suspensions were prepared.

[0054] (3) Acquisition of bacterial embedding solution

[0055] Weigh 11.7g of polyvinyl alcohol, add 25ml of ultrapure water and heat to dissolve for 15min, take it out and stir evenly, then heat for 5min, take it out, stir evenly and cool to room temperature, mix 25ml of polyvinyl alcohol solution with 100ml of the prepared bacterial suspension After mixing, the mass concentration of anammox bacteria was 2%, and 5.2g of calcium carbonate was added, and an appropriate amount of ultrapure water was added, and 130ml of bacterial embedding solutio...

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Abstract

The invention discloses a method for preparing a bioactive filler through immobilization of an anaerobic ammonia oxidizing bacterium, and belongs to the field of water treatment. The active filler is prepared by the following steps of: grinding active carbon, soaking in an anaerobic ammonia oxidizing bacterium liquid culture medium, and preparing an embedding solution from polyvinyl alcohol, calcium carbonate and condensed anaerobic ammonia oxidizing bacterium liquor in proportion; mixing and uniformly stirring the prepared embedding solution with soaked granular active carbon, and implementing immobilization through boric acid to finish cross-linking immobilization, thereby obtaining the active filler. The bioactive filler, after being cultured for 2 weeks, can recover anaerobic ammonia oxidizing activity. The bioactive filler disclosed by the invention is good in settlement performance and high in mechanical strength, and can achieve a micro-expansive state in an upflow biofilter.

Description

technical field [0001] The invention relates to the field of water treatment, in particular to a method for immobilizing anaerobic ammonia oxidation bacteria to prepare biologically active fillers. Background technique: [0002] Anaerobic ammonium oxidation technology (ANAMMOX) is currently known as the most economical biological denitrification pathway. Studies by Strous et al. at Delft University in the Netherlands have shown that the biological reaction process including cell synthesis in the anaerobic ammonium oxidation process can be expressed as the formula ( 1) [0003] NH 4 + +1.32NO 2 -+0.066HCO 3 - +0.13H + → [0004] 1.02N 2 +0.26NO 3 -+0.066CH 2 o 0.5 N 0.5 +2.03H 2 O (1) [0005] From the biological reaction characteristics of ANAMMOX, it can be seen that compared with the traditional nitrification and denitrification technology, ANAMMOX has the advantages of low oxygen demand, low operating cost and no additional carbon source. [0006] Due to th...

Claims

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Application Information

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IPC IPC(8): C02F3/28
Inventor 杨宏陶慕翔张长利吴宣顾运杜强强胡希佳尚海源赵月兰王玉洁姚仁达
Owner BEIJING UNIV OF TECH
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