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Lycorine alkaline compound with neuroprotective effect

A technology of neuroprotection and compounds, applied in the field of medicine, can solve the problems of unclear pathogenic mechanism, treatment, and failure to achieve therapeutic effect of AD

Inactive Publication Date: 2013-07-10
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, since the pathogenic mechanism of AD is not yet clear, the therapeutic drugs for AD can only improve the symptoms of AD patients, but cannot treat the cause of AD, so no good therapeutic effect has been achieved.

Method used

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  • Lycorine alkaline compound with neuroprotective effect
  • Lycorine alkaline compound with neuroprotective effect
  • Lycorine alkaline compound with neuroprotective effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of embodiment 1 hybrid lycorine

[0032] 18 kg of fresh bulbs of Hybridized Lycoris were chopped, heated with 95% ethanol for 5 times (2 hours each time), the extracts were combined (about 15 L), concentrated under reduced pressure to remove most of the ethanol, and the extract was evenly dispersed in water. Adjust the pH value of the aqueous phase to 2, 10 and 12 with dilute hydrochloric acid and sodium hydroxide solution, and extract with dichloromethane to obtain three dichloromethane sites: 173.8g (when the aqueous phase pH=2), 22.7g (the aqueous phase pH=10) and 12.06g (water phase pH=12).

[0033]The dichloromethane pH 10 extraction fraction (water phase pH=10, 22.7 g) was dissolved in methanol and then subjected to gel chromatography and thin layer chromatography to detect and combine the same fractions to obtain 6 fractions (I-VI). Part III crude silica gel mixed sample, 200g silica gel column chromatography, dichloromethane:methanol 1:0 ...

Embodiment 2

[0034] Example 2 Hybrid lycorine to H 2 o 2 Protective effect on induced neuronal injury

[0035] The results showed that the hybrid lycorine on H 2 o 2 Induced SH-SY5Y nerve cell injury has a protective effect. At 12.5, 25, and 50 μM concentrations, there was a very significant difference between the cell survival rate of the administration group and the model group (P2 o 2 Induced neuronal damage.

[0036] 1. Experimental materials

[0037] 1.1 Test sample

[0038] Hybridized lycorine was dissolved in DMSO (sigma) and configured as a 10000 μM stock solution, and then diluted to the required concentration with DMEM medium.

[0039] 1.2 Cell lines

[0040] SH-SY5Y (human neuroblastoma cells)

[0041] 1.3 Culture medium

[0042] DMEM+10%NBS+double antibody

[0043] 1.4 Other instrument materials

[0044] BioTek-Synergy2 multifunctional microplate reader, cell culture incubator, ultra-clean bench, 96-well culture plate, cell culture flask, etc. 30%H 2 o 2 Purchase...

Embodiment 3

[0052] Example 3 Hybridization of lycorine to CoCl 2 Protective effect on induced neuronal injury

[0053] The results showed that the hybrid lycorine had a positive effect on CoCl 2 Induced SH-SY5Y nerve cell injury has a protective effect. At concentrations of 12.5, 25, 50, and 100 μM, there were extremely significant differences between the cell survival rate of the administration group and the model group (P2 Induced neuronal damage.

[0054] 1. Experimental materials

[0055] 1.1 Test sample

[0056] ditto

[0057] 1.2 Cell lines

[0058] ditto

[0059] 1.3 Culture medium

[0060] ditto

[0061] 1.4 Other instrument materials

[0062] Ditto. CoCl 2 Purchased from sigma, prepared to a concentration of 300μM when used, freshly prepared before each use.

[0063] 2. Experimental method

[0064] MTT method: 3-4×10 4 cells / ml of cell suspension 92 μL, placed at 37 ° C, 5% CO 2 Inside the incubator. After 22 hours of cell attachment, add the sample to be tested, ...

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Abstract

The invention belongs to the technical field of medicine, relates to amaryllidaceae alkaloids, and particularly provides a lycorine alkaline compound extracted from hybridized lycoris (a hybrid product of L. aurea and L. radiata). The compound has a protective effect on nerve cells, has prevention and treatment effects on Alzheimer's disease, and can be used for preparation of neuroprotective agents and drugs for preventing Alzheimer's disease.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the protective effect of a novel amaryllidaceae alkaloid compound (hybrid lycorine) on nerve cells and its application in preventing and treating Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD) is a primary degenerative disease of the central nervous system, with more than 36.5 million people suffering from it worldwide. The disease mainly manifests as a progressive decline in intelligence, memory and cognition, and patients gradually lose their ability to think, speak and move towards death. There are about 8 million AD patients in my country. Because advanced AD patients cannot take care of themselves and are often accompanied by severe mental and neurological symptoms, it not only seriously reduces the quality of life of the elderly, but also brings a heavy mental and economic burden to the family and society. AD, as the main type of s...

Claims

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Application Information

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IPC IPC(8): C07D491/16A61K31/4745A61P25/28
Inventor 阮汉利
Owner HUAZHONG UNIV OF SCI & TECH
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