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Raja porosa cartilage polypeptide angiogenesis inhibitory factor, and preparation method and application thereof

An angiogenesis-inhibiting, polypeptide-based technology, applied in the field of ray cartilage polypeptide-based angiogenesis-inhibiting factors, to achieve high activity, scientific and reasonable technology, and the effect of inhibiting angiogenesis

Inactive Publication Date: 2013-07-17
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the applicant found that the technology research on the preparation of polypeptide angiogenesis inhibitors by enzymatic hydrolysis technology using the cartilage of Kong Ray cartilage as raw material is still in a blank stage, while the preparation of highly active polypeptide angiogenesis inhibitors and its The application has not been reported

Method used

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  • Raja porosa cartilage polypeptide angiogenesis inhibitory factor, and preparation method and application thereof
  • Raja porosa cartilage polypeptide angiogenesis inhibitory factor, and preparation method and application thereof
  • Raja porosa cartilage polypeptide angiogenesis inhibitory factor, and preparation method and application thereof

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Embodiment 1

[0034] 1) Using the cartilage of the ray cartilage as the raw material, add it to the guanidine hydrochloride solution with a concentration of 1.0 mol / L (containing 0.02 mol / L MES and 0.02 mol / L EDTA, pH 7.6) at a solid-to-liquid ratio of 1 g: 5 mL. Shake and extract at 4°C for 48 h, and the extracted solution was kept below 4°C, centrifuged at 4000 r / min for 20 min, and the residue was removed to collect the supernatant; the supernatant was centrifuged at 12000 r / min for 25 min at Supernatant: Put the supernatant into a dialysis bag with a molecular weight cut-off of 3 kDa, dialyze with Tris-HCl (0.02 mol / L, pH 7.6) buffer at 4°C for 24 h, and replace it every 6 h Tris-HCl (0.02 mol / L, pH 7.6) buffer solution once, the solution in the dialysis bag is the crude extract of cartilage of the ray cartilage;

[0035] 2) The crude cartilage extract of Ray Kong ray was pre-cooled at 4 °C for 1 h, slowly added acetone pre-cooled at 4 °C to a concentration of 30%, and after standing fo...

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Abstract

The invention discloses a raja porosa cartilage polypeptide angiogenesis inhibitory factor and a preparation method and application thereof. The amino acid sequence of the polypeptide angiogenesis inhibitory factor is Gly-Glu-Glu-Gly-Thr-Met-Gly-Leu (GEEGTMGL), and in ESI / MS detection, a molecular ion peak of m / z 793.11 Da ([M+H]<+>) is given out; the raja porosa cartilage polypeptide angiogenesis inhibitory factor Gly-Glu-Glu-Gly-Thr-Met-Gly-Leu (GEEGTMGL) can effectively inhibit angiogenesis of a chick chorioallantoic membrane (CAM) and proliferation of a human lung cancer cell A-549 and a human hepatoma cell line Bel-7402. The raja porosa cartilage polypeptide angiogenesis inhibitory factor Gly-Glu-Glu-Gly-Thr-Met-Gly-Leu (GEEGTMGL) can be used for preparation of drugs for inhibiting angiogenesis and preventing and treating tumors.

Description

technical field [0001] The invention relates to angiogenesis inhibitory factor of ray cartilage polypeptide, and also relates to a preparation method of the inhibitory factor of angiogenesis polypeptide of ray cartilage, and an application of the inhibitory factor of polypeptide angiogenesis of ray cartilage. Background technique [0002] Since Folkman, a professor of Harvard Medical School in the United States, first proposed the idea that "tumor growth depends on angiogenesis" in the 1970s, the relationship between tumor angiogenesis and tumor growth and metastasis has attracted widespread attention. Many studies have shown that angiogenesis is a dynamic and coordinated complex process involving a variety of cytokines, involving a series of morphological changes (such as endothelial cell activation, degradation of basement membrane, directional movement and proliferation of endothelial cells, new microvascular , new basement membrane synthesis of endothelial cells, gener...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/18C07K1/16C12P21/06A61K38/08A61P9/00A61P35/00
Inventor 王斌胡发远罗红宇徐银峰
Owner ZHEJIANG OCEAN UNIV
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