Electrochemical immunosensor making method and Streptococcus suis detection method using electrochemical immunosensor

An immunosensor, Streptococcus suis technology, applied in electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problems of long detection time, high cost, unsuitable for sample detection, etc., to achieve short response time, low detection cost, The effect of simple and easy detection method

Inactive Publication Date: 2013-07-17
SOUTHWEST UNIVERSITY
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Problems solved by technology

[0004] At present, the methods used for the detection of Streptococcus suis type 2 include ELISA, PCR, enzyme-linked immunoassay, immunochromatography and agar diffusion te

Method used

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  • Electrochemical immunosensor making method and Streptococcus suis detection method using electrochemical immunosensor
  • Electrochemical immunosensor making method and Streptococcus suis detection method using electrochemical immunosensor

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Embodiment Construction

[0026] Example, the preparation method of the electrochemical immunosensor, the glassy carbon electrode is firstly treated with 1.0 μm, 0.3 μm and 0.05 μm Al 2 o 3 After the paste was polished, it was rinsed with distilled water, then ultrasonically washed in distilled water, acetone, and distilled water, and dried in the air; then the glassy carbon electrode was modified with nano-gold, washed with buffer solution PBS and dried, and 10 uL concentration of 70 ug / One mL of Streptococcus suis type 2 antibody was coated on the surface of the modified glassy carbon electrode, overnight at 4 °C, and soaked in 0.25 wt.% bovine serum albumin (BSA) solution for 2 h to obtain electrochemical immunoassay. sensor.

[0027] In this embodiment, the electrochemical immunosensor is used for the detection method of Streptococcus suis type 2 as follows:

[0028] 1) Synthesis of carboxyferrocene nanospheres (nFc). Dissolve 0.1 g of carboxyferrocene powder in 25 mL of absolute ethanol, then ...

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Abstract

An electrochemical immunosensor making method is characterized in that the making method comprises the following steps: modifying a glassy carbon electrode by nano-gold, washing with a buffer solution, airing, coating the surface of the glassy carbon electrode by one droplet of a Streptococcus suis type 2 antibody, staying at 4DEG C overnight, and immersing the obtained glassy carbon tube in a bovine serum albumin solution to obtain an electrochemical immunosensor. The electrochemical immunosensor has a simple making method and is convenient to use, and the electrochemical immunosensor type 2 detection method using the electrochemical immunosensor has the advantages of simplicity, easy implementation, high sensitivity, short response time, low detection cost, accurate and reliable detection result, and creation of important conditions for the diagnosis and treatment of cardiovascular diseases.

Description

technical field [0001] The invention relates to an electrochemical immunosensor, in particular to a preparation method of the electrochemical immunosensor and a method for detecting the type 2 antigen of Streptococcus suis. Background technique [0002] Streptococcus suis (S. suis) is an important zoonotic pathogen, manifested as acute hemorrhagic sepsis, endocarditis, meningitis, arthritis, diarrhea in suckling piglets and abortion in pregnant pigs. Streptococcus suis infection can not only cause swine septicemia pneumonia, meningitis, arthritis and endocarditis, but also can infect specific populations and cause death, which is very serious. The pathogen of Streptococcus suis has existed in pigs for a long time, because changes in the external environment have caused the pathogen to mutate, thereby breaking through the population barrier and infecting humans through wounds and digestive tracts. According to the different antigen components of capsular polysaccharide (CPS)...

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Application Information

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IPC IPC(8): G01N27/26G01N33/569
Inventor 苏会岚袁若
Owner SOUTHWEST UNIVERSITY
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