Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

DHA (docosahexaenoic acid) liposome preparation method

A liposome and lipid technology, which is applied in the directions of liposome delivery, food preparation, medical preparations containing active ingredients, etc. Narrow, stable quality, small average particle size effect

Inactive Publication Date: 2013-08-07
OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although their preparation processes are simple, they all have the common disadvantage that lipid components such as phospholipids need to be dissolved in organic solvents, and the encapsulation efficiency of the preparations is low, so they are not suitable for large-scale industrial production.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DHA (docosahexaenoic acid) liposome preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Weigh 1.00g DHA, 4.00g lecithin, 1.00g cholesterol, 1.50g Tween-80 and 0.20g vitamin E respectively by weight, dissolve them in 80ml absolute ethanol in a 55°C water bath, and then The organic solvent was removed by rotary evaporation. Then add 91ml of distilled water, wash the membrane by rotary evaporation for 20 min, and finally adjust the volume to 100ml. Add the crude lipid suspension into a dynamic high-pressure processor, and perform ultramicro-emulsification treatment at 120MPa for 3 times to prepare DHA liposomes. The DHA encapsulation efficiency of the prepared liposomes was 69.65%, and the average particle size was 83.8 nm.

Embodiment 2

[0015] Weigh 1.00g DHA, 4.00g lecithin, 1.00g cholesterol, 1.00g Tween-80 and 0.20g vitamin E respectively by weight, dissolve them in 80 ml absolute ethanol in a water bath at 55°C, and then The organic solvent was removed by rotary evaporation. Then add 91ml of distilled water, wash the membrane by rotary evaporation for 20min, and finally adjust the volume to 100ml. Add the crude lipid suspension into a dynamic high-pressure processor, and perform ultramicro-emulsification treatment at 120MPa for 3 times to prepare DHA liposomes. The DHA encapsulation efficiency of the prepared liposome was 67.61%, and the average particle size was 102.1nm.

Embodiment 3

[0017] Weigh 1.00g DHA, 2.00g lecithin, 0.5g cholesterol, 0.50g Tween-80 and 0.10g vitamin E respectively by weight, dissolve them in 80ml absolute ethanol in a water bath at 55℃, and then The organic solvent was removed by rotary evaporation. Then add 91ml of distilled water, wash the membrane by rotary evaporation for 20min, and finally adjust the volume to 100ml. The crude lipid suspension was added to a dynamic high pressure machine, and subjected to ultramicro-emulsification treatment at 120MPa for 3 times to prepare DHA nanoliposomes. The DHA encapsulation efficiency of the prepared liposome was 52.23%, and the average particle size was 89.6nm.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
The average particle sizeaaaaaaaaaa
The average particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a DHA (docosahexaenoic acid) liposome preparation method. By taking fat-soluble DHA as a raw material, taking lecithin and cholesterol as membrane materials and combining the traditional membrane method and a novel dynamic high-pressure method, the method comprises the following steps: dissolving, evenly mixing, performing reduced pressure evaporation, and performing dynamic high-pressure treatment and other treatment operations to prepare DHA liposome. The DHA encapsulation rate of the liposome is 50-70%; the average particle size is 80-160nm; and the product has favorable storage stability.

Description

technical field [0001] The invention relates to a preparation method of DHA liposome. Background technique [0002] DHA (Docosahexaenoic acid, docosahexaenoic acid) is a polyunsaturated fatty acid, which is high in deep sea fish oil. DHA has a variety of physiological functions, such as inhibiting platelet aggregation, reducing thrombus formation, preventing cardiovascular diseases; strengthening the brain and improving intelligence; protecting eyesight; anti-allergic effects; inhibiting tumor growth, reducing the incidence of cancer; lowering blood fat, preventing arteriosclerosis, etc. However, due to its six double bonds, it is easily affected by oxygen, light, and heat, and its odor, water solubility, and fluidity are poor, which greatly limits its application. Therefore, how to effectively protect DHA has always been a difficult problem. Aiming at the special physiological function of the food nutrient DHA and the characteristics of liposomes, liposome embedding techn...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61P35/00A61P27/02A61K9/127A61P9/00A61K31/202A61P3/06A61P7/02A23K1/16A61P9/10A61P37/08A23L1/29A23L33/00
Inventor 杨水兵胡亚芹余海霞任西营胡庆兰
Owner OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com