Marek's disease virus rapid combined-detection test strip

A technology of Marek's disease and test strips, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as difficult popularization and application, unsuitable for production or rapid on-site diagnosis, complicated operation of molecular biology detection methods, etc., and it is not easy to achieve Misjudgment of false negatives and false positives, intuitive result display, and intuitive result judgment

Inactive Publication Date: 2013-08-07
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Similar to virus isolation and culture, molecular biology detection methods are complicated to operate and require special instruments and professional technicians
Although the serological method is relatively simple, it still requires fluorescence microscope, microplate reader and many supporting reagents, as well as more complicated operation steps and laboratory working environment, which is not suitable for rapid diagnosis in production or on-site. Or veterinary clinical promotion and application

Method used

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  • Marek's disease virus rapid combined-detection test strip
  • Marek's disease virus rapid combined-detection test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: A test strip for the rapid detection of Marek's disease virus (MDV), see figure 1 , figure 2 . The support layer 1 is made of plastic sheet strips, the sample adsorption fiber layer 2 is made of glass wool, and the gold-labeled antibody fiber layer 3 is attached with colloidal gold-labeled anti-MDV monoclonal antibody made of glass wool. The plain film layer 4 is made of nitrocellulose, and the water-absorbing layer 5 is made of water-absorbing filter paper. The layers numbered 2, 3, 4, and 5 are pasted on the support layer 1 in sequence, and the fibers at the joint joints intersect and penetrate each other. The cellulose membrane layer 4 is provided with an anti-MDV polyclonal antibody IgG solution to mark a detection blot 6 (code T), and a goat (rabbit) anti-mouse IgG solution to mark a control blot 7 (code C); the detection blot and the control blot are arranged in the form of " ｜｜ ". The test end protective film 8-1 is covered on the sample adsorpt...

Embodiment 2

[0050] Example 2: The test strip for detecting Marek's disease virus is basically the same in structure and preparation method as in Example 1, except that the anti-MDV gold standard marked with colloidal gold is attached to the gold-labeled antibody fiber layer 3 made of nylon fibers Polyclonal antibody; on the cellulose membrane layer 4 made of nitrocellulose, there is a detection blot T sprayed with an anti-MDV monoclonal antibody IgG solution, and a control blot C sprayed with a goat (rabbit) anti-mouse IgG solution. Others including test sample preparation, operation method and result judgment are the same as in Example 1 and will not be repeated.

Embodiment 3

[0051] Example 3: The test strip for detecting Marek's disease virus is basically the same in structure and preparation method as in Example 1, except that the anti-MDV gold-labeled monoclonal antibody is attached to the gold-labeled antibody fiber layer 3 made of polyester fiber ; On the cellulose membrane layer 4 made of polyvinylidene fluoride, the detection blot was marked with the corresponding anti-MDV polyclonal antibody IgG solution, and the control blot C was printed with the goat (rabbit) anti-mouse IgG solution.

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Abstract

The invention discloses a test strip used in rapid detection of Marek's disease virus (MDV). The test strip comprises a non-absorbing supporting layer, and an absorption layer adhered to the supporting layer. The absorption layer is formed by sequentally spliced components of an absorption fiber layer, a gold-labeled antibody fiber layer, a cellulose film layer, and a water absorption layer. The cellulose film layer is marked with anti-goat IgG or anti-mouse IgG control blot, and a detection blot comprising anti-MDV antibody. The anti-MDV antibody is an anti-MDV monoclonal or polyclonal antibody. An anti-MDV polyclonal antibody or monoclonal antibody marked by colloidal gold and corresponding to the detection blot is adhered to the gold-labeled antibody fiber layer. The test strip provided by the invention has the advantages of high detection specificity, high sensitivity, simple operation, fast detection, intuitive and accurate result, and low detection cost. The test strip is suitable for MDV virus on-site rapid detection. The test strip can be widely applied, and is suitable for popularization.

Description

technical field [0001] The invention relates to a device for detecting Marek's disease, in particular to a colloidal gold test strip for rapidly detecting Marek's disease virus. technical background [0002] Marek's disease (MD) is a highly contagious, contagious, lymphoproliferative neoplastic disease of poultry caused by Marek's disease virus (MDV). The disease is characterized by mononuclear cell infiltration and tumor formation in the peripheral nerves, gonads, iris, various internal organs, muscles, and skin. Clinically, MD-affected chicken flocks show symptoms such as uneven growth, extreme emaciation, and death. The most common lesions are nerve damage and lymphatic tumors of various solid organs, with liver and spleen tumors being the most common. Asymmetric progressive paralysis, eventually leading to complete paralysis of one or more limbs. According to the symptoms and the location of the lesion, MD is clinically divided into four types, namely neurological type...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/532
Inventor 罗俊滕蔓张改平崔治中邓瑞广江国托邢广旭赵鹏杨继飞郅玉宝杨艳艳郭军庆王方雨乔松林王丽
Owner HENAN ACAD OF AGRI SCI
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