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Method for inducing endophytic fungi of orchid

A technology of plant endophytic fungi and plants, applied in botany equipment and methods, horticultural methods, applications, etc., can solve the problems of no breakthrough progress, high cost, cumbersome production steps, etc., and achieve excellent space volume, The effect of low cost and high seed emergence rate

Active Publication Date: 2013-08-14
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the fact that the seed bag cannot meet the demand, and the particularity of the material selection of the seed bag makes the production steps cumbersome and the cost is too high, there will be many problems in the observation and recording
Therefore, in recent years, there has been no breakthrough in the research on in situ symbiotic germination of orchid seeds at home and abroad.

Method used

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  • Method for inducing endophytic fungi of orchid
  • Method for inducing endophytic fungi of orchid
  • Method for inducing endophytic fungi of orchid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Low temperature treatment of fruit pods

[0027] Select strong and excellent Baiji plants for artificial hybridization and bagging treatment. After 18 weeks of pollination, pick the plump and mature Baiji pods, put the picked pods into kraft paper bags, and place them in a ventilated and cool place Dry it until the color of the pods turns brown and the shell of the pods is completely dehydrated, and store them in the refrigerator at 4°C.

[0028] 2. Making seedling cups

[0029] Such as figure 1 As shown, select a 450mL transparent and colorless disposable plastic cup, cut a single layer of 40-mesh nylon mesh according to the caliber of the plastic cup mouth, and cover the plastic cup mouth, lock it with a paper clip, and then use scissors to remove the plastic cup. Bottom of the cup.

[0030] 3. Seed sowing

[0031] Use a blade to plan the pods of the pods after the low-temperature treatment in step 1 along the longitudinal ribs, take out the seeds in the pods, ...

Embodiment 2

[0037] 1. Low temperature treatment of fruit pods

[0038] Robust Dendrobium officinale plants with excellent traits were selected for artificial hybridization and bagged. After 18 weeks of pollination, the full and mature pods of Dendrobium officinale were picked and stored in a refrigerator at 4 °C.

[0039] 2. Making seedling cups

[0040] Select a 450mL transparent and colorless disposable plastic cup, cut a single layer of 60-mesh nylon mesh according to the caliber of the mouth of the plastic cup, and cover the mouth of the plastic cup, lock it with a paper clip, and remove the bottom of the plastic cup with scissors.

[0041] 3. Seed sowing

[0042] Use a blade to plan the pods of Dendrobium officinale after the low-temperature treatment in step 1 along the longitudinal ribs, take out the seeds in the pods, mix the seeds and plant ash at a volume ratio of 1:1000, and turn the seedling cup prepared in step 2 upside down , use a brush to pick up the mixed Dendrobium off...

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Abstract

The invention discloses a method for inducing endophytic fungi of orchid. The method includes: utilizing a seeding cup to replace a seed bag to perform in-situ symbiosis germination of orchid seed and induce effective endophytic fungi. The method solves the problems about seed clot, low germination rate, proneness to being influenced by weather conditions and the like caused during conventional in-situ symbiosis germination of the orchid. The method is simple to operate, convenient to get materials, low in cost and high in seed emergence rate, seedlings in different growing stages can be selected to perform effective strain separation according to requirements, and a new operable way for protecting endangered orchid and culturing seeding is developed.

Description

technical field [0001] The invention relates to a container seedling raising technology for in-situ symbiotic germination of orchid plants, and opens up a new feasible way for the protection and cultivation of seedlings of endangered orchid plants. Background technique [0002] Orchidaceae is a very large family, second only to Compositae in angiosperms. To some extent, the protection of orchids means the future protection of the earth's biodiversity. Orchids have a huge number of seeds, generally there are tens of thousands of seeds in each capsule, up to 400,000 seeds. However, the seeds of Orchidaceae are small, without endosperm, and only have incompletely developed proembryos. Under natural conditions, the nutrients required for seed germination must be provided by symbiotic fungi. Usually for the germination of Orchidaceae seeds, non-symbiotic culture is carried out by means of aseptic seed germination, and the germination rate is very high. Survival rate is low. A...

Claims

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Application Information

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IPC IPC(8): A01G7/00A01G31/02
CPCY02P60/21
Inventor 肖娅萍吕鼎豪牛俊峰王喆之任倩俐
Owner SHAANXI NORMAL UNIV
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