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Method for inducing endophytic fungi of orchid

A technology of plant endophytic fungi and plants, applied in botany equipment and methods, horticultural methods, applications, etc., can solve the problems of high cost, no breakthrough progress, cumbersome production steps, etc., and achieve low cost and high quality The effect of space volume and high seed emergence rate

Active Publication Date: 2014-04-23
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the fact that the seed bag cannot meet the demand, and the particularity of the material selection of the seed bag makes the production steps cumbersome and the cost is too high, there will be many problems in the observation and recording
Therefore, in recent years, there has been no breakthrough in the research on in situ symbiotic germination of orchid seeds at home and abroad.

Method used

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  • Method for inducing endophytic fungi of orchid
  • Method for inducing endophytic fungi of orchid
  • Method for inducing endophytic fungi of orchid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Low temperature treatment of fruit pods

[0027] Select robust and good-character Baiji plants for artificial hybridization and bagging. After 18 weeks of pollination, pick the mature Baiji fruit pods, and put the picked fruit pods into kraft paper bags and place them in a ventilated and cool place. Dry until the color of the pod changes to brown and the shell of the pod is completely dehydrated, and store it in the refrigerator at 4°C.

[0028] 2. Make a nursery cup

[0029] Such as figure 1 As shown, select a 450mL transparent and colorless disposable plastic cup, cut a single layer of 40 mesh nylon net according to the caliber of the plastic cup mouth, and cover it with a paper clip, and then use scissors to remove the plastic cup The bottom of the cup.

[0030] 3. Seed sowing

[0031] Use a razor blade to plan the berry pod after the low temperature treatment in step 1 along the longitudinal ribs, take out the seeds in the pod, mix the seeds and plant ash at a volume rat...

Embodiment 2

[0037] 1. Low temperature treatment of fruit pods

[0038] Select robust and good-character Dendrobium candidum plants for artificial hybridization and bagging treatment. After 18 weeks of pollination, pick the full-grown Dendrobium candidum pods and store them in a refrigerator at 4°C.

[0039] 2. Make a nursery cup

[0040] Choose a 450mL transparent and colorless disposable plastic cup, cut a single layer of 60 mesh nylon net according to the caliber of the plastic cup mouth, and cover the plastic cup mouth with a paper clip, and then use scissors to remove the bottom of the plastic cup.

[0041] 3. Seed sowing

[0042] Use a blade to plan the Dendrobium candidum fruit pods after the low temperature treatment in step 1 along the longitudinal ribs, take out the seeds in the pods, mix the seeds and plant ash at a volume ratio of 1:1000, and turn the seedling cup made in step 2 upside down , Dip the Dendrobium officinale seeds with a brush and mix well, press 3 grains / cm 2 The density ...

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Abstract

The invention discloses a method for inducing endophytic fungi of orchid. The method includes: utilizing a seeding cup to replace a seed bag to perform in-situ symbiosis germination of orchid seed and induce effective endophytic fungi. The method solves the problems about seed clot, low germination rate, proneness to being influenced by weather conditions and the like caused during conventional in-situ symbiosis germination of the orchid. The method is simple to operate, convenient to get materials, low in cost and high in seed emergence rate, seedlings in different growing stages can be selected to perform effective strain separation according to requirements, and a new operable way for protecting endangered orchid and culturing seeding is developed.

Description

Technical field [0001] The present invention relates to a container seedling breeding technology for the in-situ symbiosis and germination of orchid plants, which opens up a new feasible way for the protection and seedling cultivation of endangered orchid plants. Background technique [0002] Orchid plants are a very large family, second only to Compositae in angiosperms. To a certain extent, the protection of orchids also means the future protection of the earth's biodiversity. Orchid plants have a huge number of seeds. Generally, there are tens of thousands of seeds in each capsule, up to 400,000 seeds. However, orchids have small seeds, no endosperm, and only incompletely developed proembryos. The nutrients needed for seed germination under natural conditions must be provided by symbiotic fungi. Generally, for the germination of orchid seeds, non-symbiotic culture is carried out by aseptic germination of seeds, and the germination rate is high. However, in the process of see...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00A01G31/02
CPCY02P60/21
Inventor 肖娅萍吕鼎豪牛俊峰王喆之任倩俐
Owner SHAANXI NORMAL UNIV
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