Industrial production process of eimeria coccidiosis attenuated vaccine

Abstract

The invention discloses an industrial production process of eimeria coccidiosis attenuated vaccine. The industrial production process of the eimeria coccidiosis attenuated vaccine comprises the following steps of: preparation of oocyst-containing chicken manure, high-efficiency mixing, relay screening, continuous flow centrifugal and crude separation, saturated salt water subdivision, centrifugal desalination, oocyst disinfection, sporogony, oocyst counting, oocyst proportioning, microencapsulation process and package. The industrial production process disclosed by the invention is applied to industrial production of eimeria coccidiosis attenuated polyvalent vaccine liquid and solid preparation, the vaccine production efficiency is greatly improved, the requirements on scale production can be met, and the popularization and the application of products are facilitated.

Description

technical field [0001] The invention relates to a technique for isolating and purifying Eimeria oocysts from chicken infectious feces and preparing live oocyst vaccines, in particular to an industrial production process for chicken Eimeria attenuated vaccines. Background technique [0002] The preparation of Eimeria precoccidia attenuated vaccine depends on the separation of oocysts from infected feces, and the separation and purification of coccidia oocysts is a very complicated task. The existing production process is transplanted to the separation technology of the laboratory, and the oocysts are separated by floating in saturated salt water. The main principle is: since the density of coccidia oocysts (1.103-1.064) is between that of water and saturated brine, when the oocyst solution containing impurities is centrifuged in water, oocysts and a small amount of substances It sinks together when it is denser than water, and due to buoyancy, impurities with a lower density...

AI Technical Summary

Problems solved by technology

However, the traditional vaccine manufacturing technology is not suitable for industrial production, and there is an urgent need for technology suitable for large-scale production

The invention patent "Batch preparation method of Eimeria chicken coccidia oocysts" (patent number: 2011100291271.6) discloses the batch separation and purification technology of coccidia oocysts, which is one of the key technologies for the production of chicken coccidia vaccines, but it cannot solve the problem of vaccine preparation The whole process, especially the vaccine solid preparation

[0010] This production process is actually a common method transplanted in the laboratory, and its shortcomings are obvious: ①The efficiency of sieving and filtering is low, and the sieving process is divided into two processes, which is time-consuming and laborious; moreover, non-mechanized sieving cannot produce enough " Screening force", the screening effect is poor

② The number of equipment required for centrifugation is too large or the time-consuming is too long. As previously analyzed, conventional centrifugation technology is not suitable for processing a large amount of materials, and even the use of ultra-large-capacity centrifugation equipment cannot achieve the effect of continuous flow centrifugation

③Lack of microencapsulation treatment of oocysts, only liquid dosage forms can be prepared, which limits the use and effect of vaccines

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