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Method for extracting antioxidant polysaccharide of kelp

A technology of oxidized polysaccharides and extraction methods, applied in the field of extraction of kelp antioxidant polysaccharides

Inactive Publication Date: 2013-08-21
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to the characteristic physical and chemical properties of polysaccharides, there are few reports on the establishment of characteristic polysaccharide preparation methods

Method used

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  • Method for extracting antioxidant polysaccharide of kelp
  • Method for extracting antioxidant polysaccharide of kelp
  • Method for extracting antioxidant polysaccharide of kelp

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) The kelp is desanded, washed, dried, and crushed to 20 meshes to extract raw materials;

[0032] (2) Add 20 times the weight of distilled water to the extracted raw materials, extract at 125°C for 2 hours, filter and remove the supernatant, and separate to obtain kelp slag;

[0033] (3) Extract polysaccharides from the kelp slag obtained in step (2) with acetic acid solvent, that is, add 40 times the dry weight of kelp slag in acetic acid solvent with pH=1.5, extract at 110°C for 4 hours, filter, and separate the solid and liquid to obtain For the polysaccharide extract, adjust the pH value of the polysaccharide extract to 6.5 with potassium hydroxide;

[0034] (4) Vacuum concentrating the polysaccharide extract obtained in step (3) to 1 / 6 of the original volume to obtain a concentrated solution;

[0035] (5) Add ethanol to the concentrated solution, mix well until the final concentration of ethanol is 70% (v / v), let it stand at 0°C for 6 hours, filter, remove the ...

Embodiment 2

[0044] (1) The kelp is desanded, washed, dried, and crushed to 40 meshes to extract raw materials;

[0045] (2) Add 30 times the weight of distilled water to the extracted raw materials, extract at 110°C for 3 hours, filter and remove the supernatant, and separate to obtain kelp slag;

[0046] (3) Extract polysaccharides from the kelp residue obtained in step (2) with a citric acid solvent, that is, add 30 times the dry weight of the kelp residue with a citric acid solvent of pH=2, extract at 125°C for 2 hours, filter, and separate solid and liquid , the polysaccharide extract, adjusting the pH value of the polysaccharide extract to 7.0 with potassium hydroxide;

[0047] (4) Vacuum concentrating the polysaccharide extract obtained in step (3) to 1 / 5 of the original volume to obtain a concentrated solution;

[0048] (5) Add ethanol to the concentrated solution, mix well until the final concentration of ethanol is 85% (v / v), let stand at 2°C for 10 hours, filter, remove the sup...

Embodiment 3

[0057] (1) The kelp is desanded, washed, dried, and crushed to 80 meshes to extract raw materials;

[0058] (2) Add 40 times the weight of distilled water to the extracted raw materials, extract at 100°C for 5 hours, filter and remove the supernatant, and separate to obtain kelp residue;

[0059] (3) Extract the polysaccharide from the kelp residue obtained in step (2) with malic acid solvent, that is, add the malic acid solvent with pH=3 according to 20 times the dry weight of the kelp residue, extract at 115°C for 3 hours, filter, and separate the solid and liquid , the polysaccharide extract, adjusting the pH value of the polysaccharide extract to 6.8 with potassium hydroxide;

[0060] (4) Vacuum concentrating the polysaccharide extract obtained in step (3) to 1 / 4 of the original volume to obtain a concentrated solution;

[0061] (5) Add ethanol to the concentrated solution, mix well until the final concentration of ethanol is 75% (v / v), let stand at 4°C for 12 hours, filt...

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Abstract

The invention discloses a method for extracting antioxidant polysaccharide of kelp. The invention discloses a novel method for extracting the functional polysaccharide of the kelp. The method is characterized in that the kelp polysaccharide with the antioxidant activity can be obtained by extracting the kelp through organic acid solvent, concentrating the mixture and precipitating the mixture through ethanol, and the kelp polysaccharide component with high acidophobe and high galactose content can be obtained by adopting column chromatography to separate the kelp polysaccharide. The kelp polysaccharide component has a unique structural characteristic as well as strong antioxidant activity.

Description

technical field [0001] The invention relates to the technical field of kelp deep processing technology, in particular to a method for extracting kelp antioxidant polysaccharides. Background technique [0002] kelp( Laminaria japonica ), named after growing on the rocks on the seabed, shaped like a belt, also known as Lunbu, Jiangbai, and medicinally called kelp. The active substances are recorded in the ancient books "Compendium of Materia Medica", "Shen Nong's Materia Medica", "Diet Therapy Materia Medica", etc., and have important edible and medicinal values. Therefore, they are often called "longevity vegetables" and "healthy foods". ", deeply loved by the people. Laminaria polysaccharide is a kind of natural biomacromolecular substances existing in the cells and cells of kelp. It has various biological functions, such as anti-oxidation, anti-tumor, anti-coagulation, immune function regulation, etc., and the various health effects of kelp. are closely related, so it is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 赵谋明崔春卢茳虹任娇艳赵海峰
Owner SOUTH CHINA UNIV OF TECH
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