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Application of silktree albizia bark water extract in preparation of anti-tumor medicaments

An anti-tumor drug, the technology of Albizia Julibrissin, applied in the direction of anti-tumor drugs, drug combinations, pharmaceutical formulas, etc.

Inactive Publication Date: 2013-08-28
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for the anticancer effect of the water extract of Albizia Julibrissin, especially through inhibition of proliferation, induction of apoptosis, and prevention of cell cycle to achieve anticancer, especially the relevant literature on anti-glioma has not seen any reports so far.

Method used

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  • Application of silktree albizia bark water extract in preparation of anti-tumor medicaments
  • Application of silktree albizia bark water extract in preparation of anti-tumor medicaments
  • Application of silktree albizia bark water extract in preparation of anti-tumor medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Preparation of Albizia Julibrissin Water Extract and Preparation of Medicinal Solution

[0023] Crush the dried bark of Albizia Julibrissin, accurately weigh 50g of the powder, boil it twice with 1000ml of distilled water for 1.5 hours each time, filter it roughly with gauze, combine the two medicinal solutions, centrifuge at 8000rpm / min for 10min, and take the supernatant for vacuum Suction filtration, the filtrate was concentrated under reduced pressure with a rotary evaporator, and finally the concentrated components were freeze-dried with a freeze dryer, and the obtained dry matter was the water extract of Albizia juliensis bark, with a yield of 10.6%.

[0024] Preparation of Albizia Julibrissin Medicinal Solution: Accurately weigh 0.1 g of dried Albizia Julibrissin aqueous extract, dissolve it in 10 mL of autoclaved PBS buffer, and after fully dissolving, it becomes 10 mg / mL Albizia Julibrissin Medicinal Solution. Sterilize by filtration with a microporo...

Embodiment 2

[0025] Example 2: Effect of Albizia Julibrissin Water Extract on the Proliferation Activity of C6 Cells

[0026] Experimental process: After the rat brain glioma C6 cell line in the logarithmic growth phase was digested with 0.25% Trypsin-0.02%Na2EDTA, it was seeded in a 96-well cell culture plate at a density of 2×105 / mL, and each well was 200 μL, put in the cell incubator for culture. After the cells were completely attached to the wall, the cell culture plate was taken out, and the pre-prepared Albizia juniper solution was added, 20 μL / well, so that the final concentrations were 0, 50, 100, 150, 200, 250, 300, and 350 μg / mL. Each group has 6 replicate wells. After reaching the time required for the experiment (24h, 48h, 72h), take out the cell culture plate, add MTT solution (5mg / mL), 20μL / well, put it in the incubator to continue the culture, and stop the culture after 4h. Carefully aspirate and discard the culture medium in the wells, add 150 μL DMSO to each well, shake...

Embodiment 3

[0032] Example 3: Apoptosis-inducing effect of Albizia Julibrissin on C6 cells

[0033] Experimental process: the Annexin V-FITC cell apoptosis detection kit produced by Nanjing Kaiji Biotechnology Co., Ltd. was used. After digesting the rat brain glioma cell line C6 in the logarithmic growth phase with EDTA-free cell hydrolysis solution, inoculate it in a 60mm cell culture dish at a density of 1.0×105 / mL, 4 mL per plate, and release the cells Incubator cultivation. After the cells were completely adhered to the wall, the culture dish was taken out, the old medium was discarded, and the pre-prepared fresh medium containing the aqueous extract of Albizia juliensis at a concentration of 0, 200, and 250 μg / mL was added, and the culture was continued for 24 hours in the incubator. Digest and collect the cells of each experimental group and control group, wash the cells twice with PBS, add 500 μL Binding Buffer to suspend the cells; add 5 μL Annexin V-FITC, flick gently, add 5 μL ...

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Abstract

The invention relates to an application of a silktree albizia bark water extract in preparation of anti-tumor medicaments. The silktree albizia bark water extract is prepared through the following method: crushing dry silktree albizia bark, performing water extraction, centrifugation, suction filtration and decompression concentration and finally freeze-drying for preparation. The obtained silktree albizia bark water extract is respectively used for performing cell survival rate experimental analysis on a variety of tumor cell lines, such as liver cancer cells HepG2, esophageal cancer cells Eca9706, glioma cells C6 and the like, and the silktree albizia bark water extract is found to have an inhibition effect against proliferation of the HepG2, the Eca9706 and the C6 cancer cell lines, have the very obvious inhibition effect against the proliferation of a C6 cancer cell strain and be capable of inducing apoptosis and preventing cell cycle effect. The silktree albizia bark water extract can be applied in the preparation of the anti-tumor medicaments.

Description

technical field [0001] The present invention relates to the use of traditional Chinese medicine extracts, in particular to the application of the water extract of Albizia Julibrissin in the preparation of anti-tumor (glioma, liver cancer, esophageal cancer) drugs. Background technique [0002] Brain glioma (glioma cell) is a common intracranial malignant tumor that occurs in adults, with an incidence rate of 5 / 100,000 (Silvia Gil., et al. Radiation Oncology, 2011, 6:37-45) , the average survival period is only 9-12 months (Ji C Jeong, et al. Journal of Experimental & Clinical Cancer Research, 2011, 30:44-51). Since glioma is a highly proliferative, highly invasive tumor with rich blood vessels, the survival rate of patients with glioma after surgical resection and postoperative adjuvant radiotherapy and chemotherapy is still low (McLendon R., et al. al. Oxford University Press, 1998). As the traditional medicine of the motherland, traditional Chinese medicine has the effec...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P35/00A61K129/00
Inventor 陈廷贵昝亚伟张立伟阴彩霞
Owner SHANXI UNIV
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