Production method of modified chitosan
A production method, chitosan technology, applied in the field of bioengineering, can solve the problems of high viscosity and low solubility of chitosan, and achieve the effect of wide water solubility, good water solubility and good safety
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[0022] Example 1 Biosynthesis and application of chitosan gelatin protein
[0023] Biosynthesis of chitosan gelatin protein: Take 100 grams of chitosan and dissolve it in 0.1-1.0 M (mol / L the same below) hydrochloric acid (or take 100 grams of chitosan hydrochloride and dissolve in water). 0.1N NaOH or 0.1N NaHCO 3 Adjust the pH of the solution to 4.0-6.2, add 1-100 grams of gelatin protein (dissolved in water in advance), add 5 grams of TG enzyme (2000 U / g), and react at 25°C-85°C for 1-48 hours. The reaction is complete After that, use 0.1N NaOH or 0.1N NaHCO 3 Adjust the pH value of the solution to 6.2-7.0, filter to remove the precipitation, dialysis or ultrafiltration desalting, spray drying or vacuum drying to obtain chitosan gelatin protein.
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[0024] Example 2
[0025] Take 100 grams of chitosan and dissolve it in 0.1-1.0 M hydrochloric acid (or take 100 grams of chitosan hydrochloride and dissolve in water), and use 0.1N NaOH or 0.1N NaHCO 3 Adjust pH 6.2. Add acyl donor gelatin protein, then add 2000 U / g transglutaminase, fully react at 25-85℃, after the reaction, use 0.1N NaOH or 0.1N NaHCO 3 Adjust pH 6.2, filter to remove precipitation, desalting, spray drying or vacuum drying; the mass ratio of chitosan or chitosan mineral acid: gelatin protein: transglutaminase is 1:0.2:0.1.
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[0026] Example 3 Biosynthesis and application of chitosan collagen
[0027] Take 100 grams of chitosan and dissolve it in 0.1-1.0 M hydrochloric acid (or take 100 grams of chitosan hydrochloride and dissolve in water), and use 0.1N NaOH or 0.1N NaHCO 3 Adjust the pH of the solution to 4.0-6.2, add 1-100 grams of collagen (dissolved in water in advance), add 5 grams of TG enzyme, and react at 25°C-85°C for 1-48 hours. After the reaction, use 0.1N NaOH or 0.1N NaHCO 3 Adjust the pH value of the solution to 6.2-7.0, filter to remove the precipitation, dialysis or ultrafiltration desalination, spray drying or vacuum drying to obtain chitosan collagen.
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