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Application of dihydromyricetin (DHM) in preparation of anti-hepatoma medicines

A technology of dihydromyricetin and anti-liver cancer, applied in the field of biomedicine, can solve the problem of no anti-liver cancer drug of dihydromyricetin, and achieve the effects of inducing apoptosis of liver cancer cells, inhibiting proliferation, and having strong anti-cancer properties.

Inactive Publication Date: 2013-09-04
HOSPITAL AFFILIATED TO GUANDONG MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no report of using dihydromyricetin as an anti-liver cancer drug

Method used

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  • Application of dihydromyricetin (DHM) in preparation of anti-hepatoma medicines
  • Application of dihydromyricetin (DHM) in preparation of anti-hepatoma medicines
  • Application of dihydromyricetin (DHM) in preparation of anti-hepatoma medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1D

[0097] Example 1 Effect of DHM on proliferation of liver cancer cell lines

[0098] 1. In vitro culture of liver cancer cell lines

[0099] 1. Cell source

[0100] Human liver cancer cell lines HepG2, Huh7, SK-HEP-1 were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences; MHcc97L, MHcc97H, QSG7701, QGY7703 were purchased from Shanghai Baili Biotechnology Co., Ltd.; QGY7701 was purchased from Shanghai Maternal and Child Health Hospital; human normal liver The cell line L-02 was purchased from Science Cell; the mouse liver cancer cell line Hepal-6 was donated by Shanghai Jiaotong University.

[0101] 2. Cell Culture and Subculture

[0102] HepG2, QSG7701, L-02 and Hepal-6 used 1640 medium (Gibco); Huh7, SK-HEP-1, MHcc97L, MHcc97H, QGY7701 and QGY7703 used DMEM medium (Gibco). 10% fetal bovine serum (fetal bovine serum, FBS, Gibco) was added to the medium during cell culture. The above cell lines were all stored at 37°C, 5% CO 2 Culture in an incubator...

Embodiment 2D

[0111] Example 2 Induction of DHM on apoptosis of liver cancer cells

[0112] 1. Cell culture and passage

[0113] In this experiment, the human liver cancer cell line QGY7701 was purchased from Shanghai Maternal and Child Health Hospital. The culture medium was DMEM culture solution supplemented with 10% FBS. 2 Culture in an incubator, and when the cells grow to 80-90% of the bottom of the culture dish, they are digested with 0.25% trypsin-EDTA and passaged.

[0114] 2. The effect of DHM on the apoptosis of liver cancer cells

[0115] Main test materials: DHM, dissolved in dimethyl sulfoxide (DMSO), prepared as a 50mM concentrated solution, stored at -20°C, diluted to 25, 50, 100 and 150μM when used, ready to use; FITC Annexin Ⅴ Apoptosis Detection Kit Ⅰ (BD Pharmingen); Accutase-Enzyme Cell Detachment Medium (eBioscience); PBS; 60mm petri dish, special sample tube for flow cytometry.

[0116] Test method: Digest the cells into a cell suspension, spread them on a 60mm cult...

Embodiment 3

[0118] Example 3 Western Blotting Detection of Cell Apoptosis-Related Factor Expression

[0119] 1. Cell culture and passage

[0120] The human liver cancer cell line HepG2 was purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences; QSG7701 and QGY7703 were purchased from Shanghai Baili Biotechnology Co., Ltd.; the mouse liver cancer cell line Hepal-6 was donated from Shanghai Jiao Tong University. HepG2, QSG7701 and Hepal-6 used 1640 medium (Gibco), and QGY7703 used DMEM medium (Gibco). 10% fetal bovine serum (fetal bovine serum, FBS, Gibco) was added to the medium during cell culture. The above cell lines were all stored at 37°C, 5% CO 2 Culture in an incubator, and when the cells grow to 80-90% of the bottom of the culture dish, they are digested with 0.25% trypsin-EDTA and passaged.

[0121] 2. Expression detection of apoptosis-related factors

[0122] Main test materials: DHM, dissolved in dimethyl sulfoxide (DMSO), prepared into a 50mM concentrated ...

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Abstract

The invention relates to the biological medicine field, and concretely relates to an application of DHM in the preparation of anti-hepatoma medicines. The DHM has a strong anticancer characteristic as a flavonoid compound. Tests prove that the DHM plays a positive role in hepatoma carcinoma cell propagation inhibition, hepatoma carcinoma cell apoptosis induction, hepatoma carcinoma cell adhesion capability reduction and hepatoma carcinoma cell invasion and transfer prevention, and can substantially reduce the contents of peroxides, glutathione and ATP in the hepatoma carcinoma cells.

Description

technical field [0001] The present invention relates to the field of biomedicine, and more specifically relates to the application of dihydromyricetin in the preparation of anti-liver cancer drugs. Background technique [0002] Liver cancer is one of the common clinical malignant tumors. There are about 600,000 new patients with liver cancer every year in the world, and the mortality rate of patients ranks third in the world. my country is a big country with hepatitis B, and primary liver cancer mostly develops on the basis of hepatitis B cirrhosis. At the same time, long-term alcoholism and bad eating habits have also been proved to be closely related to the occurrence of liver cancer. At present, the incidence of liver cancer in my country accounts for more than half of the total incidence of liver cancer in the world. Liver cancer has become a major killer that seriously threatens people's health and life, and its danger cannot be underestimated. Therefore, it is of grea...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/00
Inventor 朱润芝李明意刘洁曾国芳张庆余刘斌黄海丽
Owner HOSPITAL AFFILIATED TO GUANDONG MEDICAL COLLEGE
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