Method for immobilizing CO2 by utilizing scytonema javanicum
A Pseudomonas algae and CO2 technology, applied in separation methods, chemical instruments and methods, dispersed particle separation, etc., can solve problems such as affecting the health of workers, reducing work efficiency, endangering life and health, etc., and achieving permanent fixation and storage. , easy to operate, and the effect of reducing harm
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0042] In this embodiment, a method for fixing CO by using the biological calcification of Pseudocladidae is provided 2 The method includes the following steps:
[0043] The first step: isolation, cultivation and domestication of Pseudobranchia.
[0044] Collect samples of algae colonies with Pseudocladus algae, culture them in BG11 liquid medium, gently peel apart the algae filaments with tweezers, separate the pseudocladus algae filaments, rinse them repeatedly with sterile water and transfer them to the sterilized BG11 liquid culture Base culture.
[0045] Before use, Pseudobranchia should be domesticated with a medium close to the state of biological calcified water. The medium for domestication of Pseudobranchia is a nutrient-poor liquid medium with a composition of 5.9mg / L Ca(NO 3 ) 2 ·4H 2 O,46.7mg / L NaNO 3 ,4.1mg / LK 2 HPO 4 ·3H 2 O,2.5mg / L MgSO 4 ·7H 2 O,168mg / L NaHCO 3 , 11.45mg / L Na-EDTA (Ethylenediaminetetraacetic acid disodium), 3mg / L FeSO 4 ·7H 2 O,248μg / L H 3 BO 3 ,135μ...
Embodiment 2
[0053] In this embodiment, a method for fixing CO by using the biological calcification of Pseudocladidae is provided 2 The method includes the following steps:
[0054] After separating, culturing and domesticating Pseudobranchia, the cell fluid of Pseudobranchia in logarithmic growth phase (concentration of 1×10 5 Cells) into a flat-bottomed glass bottle (volume 1.2L), under continuous light conditions (illuminance 4000lx), add a certain concentration of NaHCO to the algal cell solution 3 Solution, then add a certain concentration of CaCl to the solution 2 Solution, make the calcium ion concentration in the flat-bottom glass bottle 190mg / L, keep the temperature at about 20℃, the initial pH of the solution is 7.5-9, and continue to pour the solution containing CO 2 Gas (CO 2 The mass concentration is 0.4%), the gas flow rate is 30ml / min, and the process continues for 100h.
[0055] After the calcification process is over, filter the above-mentioned pseudomycosa cell liquid with a 0....
Embodiment 3
[0061] In this embodiment, a method for fixing CO by using the biological calcification of Pseudocladidae is provided 2 The method includes the following steps:
[0062] After separating, culturing and domesticating Pseudobranchia, the cell fluid of Pseudobranchia in logarithmic growth phase (concentration of 1×10 8 Cells) into a flat-bottomed glass bottle (volume 1.2L), under continuous light conditions (illuminance 5000lx), add a certain concentration of NaHCO to the algal cell solution 3 Solution, then add a certain concentration of CaCl to the solution 2 Solution, make the calcium ion concentration in the flat-bottom glass bottle 150mg / L, keep the temperature at about 25℃, the initial pH of the solution is 7.5-9, and continue to pour the solution containing CO 2 Gas (CO 2 The mass concentration is 0.4%), the gas flow rate is 30ml / min, and the process continues for 100h.
[0063] After the calcification process is over, filter the above-mentioned pseudomycosa cell liquid with a 0....
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More 


