Method for detecting cryptocaryon irritans brown chemotaxis

A technology that stimulates Cryptocaryonia and measures methods, and is applied in the fields of climate change adaptation, application, fish farming, etc., and can solve problems such as cumbersome operation and inconvenient dynamic observation and counting

Inactive Publication Date: 2013-09-11
JIMEI UNIV
5 Cites 3 Cited by

AI-Extracted Technical Summary

Problems solved by technology

At present, there are methods such as plate punching method and 96-well plate method for the research on the chemotaxis of fish parasites in foreign countries. These methods have de...
View more

Abstract

The invention discloses a method for detecting cryptocaryon irritans brown chemotaxis. The method includes (1) taking a clean glass slide as a carrier, dropping 40-60 microliter of seawater droplets containing cryptocaryon irritans brown larvae on one side of the glass slide, dropping test solution with the same volume on the other side of the glass slide, and the space between the seawater droplets and the test solution is 1-2cm; (2) using a clean toothpick to draw lines gently from the seawater droplets to the test solution to make the droplets of two sides to be communicated; (3) standing for 20-40 minutes at the temperature of 25-28 DEG C, and observing the amount of the cryptocaryon irritans brown larvae moved to the test solution through a microscope and calculating the chemotaxis. Since the glass slide is adopted to detect the cryptocaryon irritans brown chemotaxis, the method is more convenient to operate as compared with the internationally conventional petri dish punching method and 96-well plate method. By the aid of the technology of detecting the cryptocaryon irritans brown chemotaxis through the glass slide, large amount of time, reagents and consumable items are saved.

Application Domain

Climate change adaptationPisciculture and aquaria

Technology Topic

CryptocaryonChemistry +10

Examples

  • Experimental program(5)

Example Embodiment

[0010] Example 1: Under the condition of 28 ℃, take a clean glass slide, drop 50 μl of the liquid that stimulates Cryptocaryon larvae on one side of the slide, and drop 50 μl of pomfret serum on the other side, With the sides 2 cm apart, line with a clean toothpick to form a channel. After 30 minutes, the number of larvae moved to the side containing the serum of Pompano ovata was found to be 35, and the total number of larvae was 55, and the chemotaxis rate was calculated to be 64% (that is, Pomfret ovata Serum has a strong chemotactic effect on stimulating Cryptocaryon larvae).

Example Embodiment

[0011] Example 2: Under the condition of 26 ℃, take a clean glass slide, drop 50 μl of liquid that stimulates Cryptocaryon larvae on one side of the slide, and drop 50 μl of mucus of pomfret larvae on the other side , the distance between the two is 1 cm, and a clean toothpick is used to draw a line to form a channel. After 30 minutes, observation and counting under the microscope revealed that the number of larvae moving to the side containing the mucus of Pomfret ovata was 21, and the total number of larvae was 60. The calculated chemotaxis rate was 35% (that is, Mucus has a certain chemotactic effect on stimulating Cryptocaryon larvae).

Example Embodiment

[0012] Example 3: Under the condition of 28 ℃, take a clean glass slide, add 40 μl of worm solution that stimulates Cryptocaryon larvae to one side of the slide, and add 40 μl of fructose solution to the other side, 2 cm apart, score with a clean toothpick to form a channel. After 30 minutes, observe and count under the microscope, it is found that the number of larvae moving to the side containing the fructose solution is 1, while the total number of larvae is 50, and the calculated chemotaxis rate is 2% (that is, the effect of fructose solution on stimulating Cryptocaryonia Larvae have no chemotaxis).

PUM

no PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products