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Method for detecting cryptocaryon irritans brown chemotaxis

A technology that stimulates Cryptocaryonia and measures methods, and is applied in the fields of climate change adaptation, application, fish farming, etc., and can solve problems such as cumbersome operation and inconvenient dynamic observation and counting

Inactive Publication Date: 2013-09-11
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are methods such as plate punching method and 96-well plate method for the research on the chemotaxis of fish parasites in foreign countries. These methods have defects such as cumbersome operation and inconvenient dynamic observation and counting.
There is no domestic research method for specifically detecting the chemotaxis of fish parasites

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Under the condition of 28 ℃, take a clean glass slide, drop 50 μl of the liquid that stimulates Cryptocaryon larvae on one side of the slide, and drop 50 μl of pomfret serum on the other side, With the sides 2 cm apart, line with a clean toothpick to form a channel. After 30 minutes, the number of larvae moved to the side containing the serum of Pompano ovata was found to be 35, and the total number of larvae was 55, and the chemotaxis rate was calculated to be 64% (that is, Pomfret ovata Serum has a strong chemotactic effect on stimulating Cryptocaryon larvae).

Embodiment 2

[0011] Example 2: Under the condition of 26 ℃, take a clean glass slide, drop 50 μl of liquid that stimulates Cryptocaryon larvae on one side of the slide, and drop 50 μl of mucus of pomfret larvae on the other side , the distance between the two is 1 cm, and a clean toothpick is used to draw a line to form a channel. After 30 minutes, observation and counting under the microscope revealed that the number of larvae moving to the side containing the mucus of Pomfret ovata was 21, and the total number of larvae was 60. The calculated chemotaxis rate was 35% (that is, Mucus has a certain chemotactic effect on stimulating Cryptocaryon larvae).

Embodiment 3

[0012] Example 3: Under the condition of 28 ℃, take a clean glass slide, add 40 μl of worm solution that stimulates Cryptocaryon larvae to one side of the slide, and add 40 μl of fructose solution to the other side, 2 cm apart, score with a clean toothpick to form a channel. After 30 minutes, observe and count under the microscope, it is found that the number of larvae moving to the side containing the fructose solution is 1, while the total number of larvae is 50, and the calculated chemotaxis rate is 2% (that is, the effect of fructose solution on stimulating Cryptocaryonia Larvae have no chemotaxis).

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PUM

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Abstract

The invention discloses a method for detecting cryptocaryon irritans brown chemotaxis. The method includes (1) taking a clean glass slide as a carrier, dropping 40-60 microliter of seawater droplets containing cryptocaryon irritans brown larvae on one side of the glass slide, dropping test solution with the same volume on the other side of the glass slide, and the space between the seawater droplets and the test solution is 1-2cm; (2) using a clean toothpick to draw lines gently from the seawater droplets to the test solution to make the droplets of two sides to be communicated; (3) standing for 20-40 minutes at the temperature of 25-28 DEG C, and observing the amount of the cryptocaryon irritans brown larvae moved to the test solution through a microscope and calculating the chemotaxis. Since the glass slide is adopted to detect the cryptocaryon irritans brown chemotaxis, the method is more convenient to operate as compared with the internationally conventional petri dish punching method and 96-well plate method. By the aid of the technology of detecting the cryptocaryon irritans brown chemotaxis through the glass slide, large amount of time, reagents and consumable items are saved.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to a method for determining chemotaxis stimulation of Cryptocaryon. Background technique [0002] With the continuous deterioration of the aquaculture environment and the gradual increase in the aquaculture density, the "white spot disease" caused by Cryptocaryon stimuli has caused huge economic losses to aquaculture marine fish. The "white spot disease" has the characteristics of high infection rate, wide spread and high mortality, and there is no good treatment method yet. The study on the chemotaxis of Cryptocaryon stimuli is helpful to the in-depth discussion on the mechanism of Cryptocaryon stimuli infecting the host, and further screens out anthelmintic drugs for the prevention and treatment of "white spot disease". At present, there are methods such as plate punching method and 96-well plate method for studying the chemotaxis of fish parasites abroad. These methods have defects su...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 鄢庆枇邹峰苏永全覃映雪马英徐晓津
Owner JIMEI UNIV
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