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Method for preparing complex enzyme-probiotic preparation for feeds through biotransformation of barley straws and tea seed cakes

A technology of probiotic preparations and barley straw, which is applied in animal feed, animal feed, applications, etc., can solve the problems of difficult digestion of monogastric animals, and achieve the effects of low-cost, high-efficiency biotransformation, and strong enzyme production capacity

Inactive Publication Date: 2014-07-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of barley straw, 5kg of tea seed cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water, at 35°C After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0028] 2) Fully mix 20kg of starter in step 1) with 600kg of barley straw, 300kg of tea seed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated, at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 55.25IU / g; total cellulase activity reaches 6.12IU / g; beta-glucosidase activity reaches 55.25IU / g; reached 12.43IU / g; endoglucanase activity reached 43.19IU / ...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of barley straw, 5kg of tea seed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0032] 2) Fully mix 20kg of starter in step 1) with 600kg of barley straw, 300kg of tea seed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated, at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0033] After measurement, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 54.98IU / g; total cellulase activity reaches 6.08IU / g; beta-glucosidase activity reaches 54.98IU / g; reached 12.36IU / g; endoglucanase activity reached 43.04IU / g; xylanas...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of barley straw, 5kg of tea seed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, at 40°C After 36 hours of acclimatization and cultivation, a leavening agent is formed;

[0036] 2) Fully mix 10kg of starter in step 1) with 600kg of barley straw, 300kg of tea seed cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and fermented at 35°C After 5 days, a compound enzyme and probiotic preparation for feed was obtained.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 54.60IU / g; total cellulase activity reaches 6.32IU / g; beta-glucosidase activity reaches 54.60IU / g; reached 13.12IU / g; endoglucanase activity reach...

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Abstract

The invention discloses a method for preparing a complex enzyme-probiotic preparation for feeds through biotransformation of barley straws and tea seed cakes. The method comprises the steps of fully mixing Puer ripe tea subjected to pile fermentation, lentinus edobes sticks, agrocybe cylindracea sticks, first barley straws, first tea seed cakes, first wheat bran and first apple pomace with first water so as to form a leaven after acclimation and culture; and fully mixing the leaven, second barley straws, second tea seed cakes, second wheat bran and second apple pomace with second water so as to obtain the complex enzyme-probiotic preparation for feeds after fermentation. The complex enzyme-probiotic preparation for feeds, with better performance indexes, is prepared by fermenting barley straws and tea seed cakes with food-grade safe floras with stable micro-ecology, thus achieving low-cost and efficient biotransformation of barley straws and tea seed cakes. Meanwhile, the method has the advantages of easiness in microorganism growth, high enzyme activity and extensive fermentation processes, and easily achieves large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting barley straw and tea seed cake by adopting micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/14
CPCY02P60/87
Inventor 阮晖吴渊伍星主纪晓燚于骅诸文颖陈功杜姗姗杨璐
Owner ZHEJIANG UNIV
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