Protein interaction detection method with low false positive rate

Abstract

The invention provides a protein interaction detection method with low false positive rate, relates to a detection system and belongs to the field of a molecular biological technology. According to the method, two generally needed bimolecular fluorescence complementation (BiFC) detection systems based on single expression plasmid vectors are built in a dual expression plasmid vector system by a BiFC technology based on fluorescent protein, so the false positive rate of the BiFC detection method in research on protein-protein interaction can be reduced obviously, and quantitative analysis can be realized. The method also can be applied to screening research on unknown protein-protein interaction based on a gene library and detection on protein-protein interaction in living animals.

Description

technical field

[0001] The invention belongs to the technical field of molecular biology, in particular to a protein interaction detection method with a low false positive rate. Background technique

[0002] The cell itself is the basic unit of an ordered life activity formed by the interaction of different biomolecules. The occurrence and regulation of major cell life activities are realized through the interaction between biomacromolecules (protein-protein, protein-nucleic acid, etc.). Protein-protein interaction (hereinafter referred to as "PPI") not only controls gene transcription, cell division and cell proliferation, but also mediates signal transduction, carcinogenesis transformation and adjustment in the process of cell life activities, so Studying protein-protein interactions has important life science significance.

[0003] At present, for mammalian cells, there are only Fluorescence Resonance Energy Transfer (FRET) technology and Bimolecular Fluorescence Comple...

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