Method for separating and purifying five purine and pyrimidine bases from trichosanthes bark

A technology of separation and purification, melon peel, which is applied in the direction of organic chemistry, can solve the problems of few types of purine and pyrimidine bases, cumbersome steps in the separation process, harm to human body and environment, etc., and achieve short process cycle, low comprehensive cost and high purity and efficiency-enhancing effects

Active Publication Date: 2013-09-18
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE +2
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Problems solved by technology

[0005] The main problem of the above-mentioned method is: the separation process step is comparatively loaded down with trivial details, needs to use chloroform sometimes as extraction agent or
[000

Method used

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  • Method for separating and purifying five purine and pyrimidine bases from trichosanthes bark
  • Method for separating and purifying five purine and pyrimidine bases from trichosanthes bark
  • Method for separating and purifying five purine and pyrimidine bases from trichosanthes bark

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Embodiment

[0037] Example: The method for separating and purifying purine and pyrimidine bases from the peel of the traditional Chinese medicine Trichosanthes quince, the steps are:

[0038] (1) Preparation of the crude extract of Trichosanthes rind: after crushing the medicinal material of Trichosanthes trichosanthes, heat and reflux extraction with water for 4 times, combine the extracts, concentrate under reduced pressure to a relative density of about 1.2, and add 95 % ethanol until the concentration of ethanol is about 70%, let it stand for 72 h, filter, take the supernatant, and concentrate under reduced pressure to obtain the crude extract.

[0039](2) Coarse separation with macroporous adsorption resin column: Dissolve the crude extract of Trichosanthes piperi in water, apply HPD826 macroporous adsorption resin column for chromatography, first elute with water, and then use ethanol with a concentration of about 5%- The aqueous solution was eluted, and water and about 5% ethanol...

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Abstract

The invention relates to a method for separating and purifying five purine and pyrimidine bases (namely cytosine, uracil, hypoxanthine, guanine and xanthine) from trichosanthes bark. According to the method, five high-purity monomeric compounds, namely cytosine, uracil, hypoxanthine, guanine and xanthine, are obtained from the trichosanthes bark through the following steps of: (1) preparing a crude trichosanthes bark extract; (2) carrying out crude separation by using a macroporous adsorption resin column; and (3) carrying out separation and purification by using semi-preparative high-performance liquid chromatography: carrying out separation and purification on a sample 1 and a sample 2 by using the semi-preparative high-performance liquid chromatography, wherein the mobile phase is methanol-water. The method disclosed by the invention has the advantages that the process flow is environment-friendly, the damage to the environment is not serious, and the comprehensive cost is low.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method for separating and purifying five kinds of purine and pyrimidine bases (cytosine, uracil, hypoxanthine, guanine and xanthine) from the skin of the traditional Chinese medicine Trichosanthes quince. Background technique [0002] Trichosanthes peel is Cucurbitaceae Trichosanthes genus Trichosanthes ( Trichosanthes kirilowii Marim.) or Bilateral Trichiola ( Trichosanthes rosthornii Harms.), which is a commonly used traditional Chinese medicine, has the effects of nourishing the lungs and reducing phlegm, benefiting qi and widening the chest, etc. It is widely used clinically to treat cough with phlegm heat, phlegm turbid yellow thick, sore throat, chest pain, hematemesis, epistaxis, Constipation, carbuncle sore swelling and toxin embolism. Modern pharmacological experiments and clinical practice have shown that Trichosanthes peel has various pharmacological ...

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Application Information

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IPC IPC(8): C07D239/47C07D239/54C07D473/30C07D473/04C07D473/18
Inventor 李爱峰张永清柳仁民孙爱玲
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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