Molecular identification method of high-digestibility sorghum strain

A Molecular Identification, High Digestibility Technology

Inactive Publication Date: 2015-04-01
ANHUI SCI & TECH UNIV
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  • Claims
  • Application Information

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Embodiment 1

[0016] Example 1: Molecular marker identification of highly digestible sorghum lines

[0017] 1. Crossing of high digestibility lines: Brown midrib sorghum line U68 (type bmr-6) was used to cross with sorghum line Tx623B.

[0018] 2. The first backcross: backcross with the hybrid as the female parent and sorghum Tx623B as the male parent to obtain BC 1 f 1 .

[0019] 3. Molecular marker identification of offspring individual plants: BC 1 f 1 20 plants were planted, DNA was extracted from individual plants, and the individual plants carrying the brown midrib gene were identified by PCR amplification.

[0020] The identification procedure is as follows:

[0021] ①. Use primer B-1 (pre-primer: GACGGCCGGCACGCG; post-primer: TTCAGACTTCAGACCAAC) to amplify the DNA of these 20 individual plants (total system: 30 μL: DNA 100ng 2mM primer 5 μL, 2 Mm dNTP 6 μL, KOD enzyme 0.5 U, 15 μL of 2-fold buffer, and make up the rest with double-distilled water. The amplification program is:...

Embodiment 2

[0026] Example 2: Molecular marker identification of high digestibility sudan grass strains

[0027] 1. Hybridization of high digestibility strains: Brown midrib sorghum line U68 (type bmr-6) was used to cross with sudangrass line S722.

[0028] 2. The first backcross: backcross with the hybrid as the female parent and Sudan grass line S722 as the male parent to obtain BC 1 f 1 .

[0029] 3. Molecular marker identification of offspring individual plants: BC 1 f 1 20 plants were planted, DNA was extracted from individual plants, and the individual plants carrying the brown midrib gene were identified by PCR amplification.

[0030] The identification procedure is as follows:

[0031] ①. Use primer B-1 (pre-primer: GACGGCCGGCACGCG; post-primer: TTCAGACTTCAGACCAAC) to amplify the DNA of these 20 individual plants (total system: 30 μL: DNA 100ng 2mM primer 5 μL, 2 Mm dNTP 6 μL, KOD enzyme 0.5 U, 15 μL of 2-fold buffer, and make up the rest with double-distilled water. The amp...

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Abstract

A molecular identification method of a high-digestibility sorghum strain belongs to a molecular breeding technology. According to the molecular identification method, the bmr-6 gene-carrying single plant can be identified and selected by utilizing a molecular marker method, so that the selection program can be simplified, the molecular identification method is easy to operate, and the breeding process can be quickened.

Description

technical field [0001] A molecular identification method for high digestibility sorghum strains belongs to sorghum molecular breeding technology, more specifically a method for identifying high digestibility sorghum genes by molecular markers. Background technique [0002] Sorghum brown midvein is a variation of leaf veins and stem xylem. Compared with ordinary white midrib sorghum, the digestibility of this mutant is increased by 40%-60%, and the palatability is significantly improved. Various grazing livestock are particularly fond of eating it, and the same feeding amount can obtain higher economic benefits. There are more than 30 types of brown midrib, and the type with the highest digestibility is bmr-6. The conventional method of breeding brown midrib (bmr-6 type) sorghum and sudangrass is to use phenotype for selection. Since brown midrib is a recessive trait, it cannot be directly identified by phenotype in the current generation of backcross. If phenotypic identif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 李杰勤王丽华詹秋文林平王茂辉
Owner ANHUI SCI & TECH UNIV
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