Method for preparing chondroitin sulfate by virtue of ultrahigh-pressure micro-jet ultrafiltration

A chondroitin sulfate and micro-fluidic technology applied in the biological field to achieve the effects of avoiding cumbersome operations, improving extraction rates, and saving ethanol consumption

Inactive Publication Date: 2013-09-25
淮北市恒通生物科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the combination of ultra-high pressure microfluidic technology and ultrafiltration technology is used to prepare chondroitin sulfate, the extr

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1

[0019] A method for preparing chondroitin sulfate by ultra-high pressure micro-jet ultrafiltration, the steps are as follows:

[0020] 1. Pretreatment: Put the cartilage raw material into boiling water and boil it for 10 minutes, remove the fat and meat residue, air dry and crush, and pass through an 80 mesh sieve to obtain cartilage powder;

[0021] 2. Ultra-high pressure micro-jet lye extraction: Add 8% NaOH solution by volume to cartilage powder. The mass ratio of cartilage powder to NaOH solution is 1:60. Place the mixture of cartilage powder and NaOH solution at room temperature. The extraction is carried out in the ultra-high pressure micro-jet extractor, the pressure is 180MPa, the extraction time is 80min, and the filtrate obtained by filtration is extraction liquid one;

[0022] 3. Enzymatic hydrolysis: adjust the pH of the extract obtained in step 2 to 8 with hydrochloric acid, add trypsin according to the enzyme mass and solution volume ratio of 1.0:5000,...

Example Embodiment

[0027] Example 2

[0028] A method for preparing chondroitin sulfate by ultra-high pressure micro-jet ultrafiltration, the steps are as follows:

[0029] 1. Pretreatment: Put the cartilage raw material into boiling water and boil for 8 minutes, remove oil and meat residue, air dry and crush, and pass through a 60-mesh sieve to obtain cartilage powder;

[0030] 2. Ultra-high pressure micro-jet lye extraction: Add 4% NaOH solution in volume percentage to cartilage powder. The mass ratio of cartilage powder to NaOH solution is 1:50. Place the mixture of cartilage powder and NaOH solution at room temperature. The extraction is carried out in the ultra-high pressure micro-jet extractor, the pressure is 80MPa~120MPa, the extraction time is 30min~60min, and the filtrate obtained by filtration is extraction liquid one;

[0031] 3. Enzymatic hydrolysis: adjust the pH of the extract obtained in step 2 with hydrochloric acid to 5, add trypsin according to the enzyme mass and solution volume rati...

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Abstract

The invention discloses a method for preparing chondroitin sulfate by virtue of ultrahigh-pressure micro-jet ultrafiltration, and the method comprises the following steps of: (I) pretreatment; (II) extraction of an ultrahigh-pressure micro-jet alkaline liquid; (III) enzymolysis; (IV) decoloration; (V) ultrafiltration concentration; (VI) ethanol washing and dehydration, and drying and grinding to obtain a finished product. The method disclosed by the invention can be used for obviously improving the extraction rate of chondroitin sulfate, wherein the extraction rate is improved to 38-40.5%, and the extraction time is reduced to 20-80 minutes; the method can be used for solving the problems in purification and concentration, achieves an aim of desalination, is simpler to operate, and can be used for overcoming the shortcomings of an existing method that a large quantity of strong base is used and the salt content is high; meanwhile, the volume of the extraction liquid is reduced to 25-50% of that of the original extraction liquid, and the ethanol use is saved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for preparing chondroitin sulfate, in particular to a method for preparing chondroitin sulfate by ultrahigh-pressure micro-jet ultrafiltration. Background technique [0002] Chondroitin Sulfate CS is a kind of acidic mucopolysaccharide widely distributed in animal cartilage and connective tissue. It has various biological activities and is mainly used for neuralgia, neuropathic migraine, arthritis, shoulder joint pain and abdominal pain. The treatment of postoperative pain and other diseases has a significant effect. [0003] There are many extraction techniques of chondroitin sulfate, mainly including: concentrated alkali extraction, dilute alkali concentrated salt extraction, dilute alkali extraction, dilute alkali-enzymatic extraction method, double enzyme method, ultrasonic assisted method, ultrasonic-microwave synergistic method, etc., among which the most Dilute alkali-...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 孔艳飞
Owner 淮北市恒通生物科技有限公司
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